A thermophilic, anaerobic, semi-continuously fed methanogenic enrichment microbiome cultivated on mid-season switchgrass at high solids loadings. The community maintained fractional carbohydrate solubilization as solids loading increased from 30 to 150 g/L, with methane and carbon dioxide as the main steady-state products. Metaproteomics identified bacterial carbohydrate-active enzyme strategies and archaeal methanogenesis functions relevant to DOE bioenergy efforts to understand robust lignocellulose deconstruction under industrially relevant process conditions.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Clostridia | NCBITaxon:186801 |
PRIMARY_DEGRADER
SECONDARY_FERMENTER
|
DOMINANT |
|
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| Bacilli | NCBITaxon:91061 |
PRIMARY_DEGRADER
SECONDARY_FERMENTER
|
ABUNDANT |
|
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| Chloroflexota | NCBITaxon:200795 |
PRIMARY_DEGRADER
SECONDARY_FERMENTER
|
COMMON |
|
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| Thermotogota | NCBITaxon:200918 |
PRIMARY_DEGRADER
SECONDARY_FERMENTER
|
COMMON |
|
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| Euryarchaeota | NCBITaxon:28890 |
SYNTROPHIC_PARTNER
CROSS_FEEDER
|
COMMON |
|
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Ecological Interactions
High-Solids Switchgrass Carbohydrate Solubilization
NICHE_PARTITIONINGSource Taxon: Clostridia
Metabolites: cellulose (CHEBI:18246), xylan (CHEBI:37686)
Biological Processes:
- cellulose catabolic process (GO:0030245)
- xylan catabolic process (GO:0045493)
Evidence
-
PMID:35790765 - SUPPORT (IN_VITRO)"undiminished fractional carbohydrate solubilization"
-
PMID:35790765 - SUPPORT (COMPUTATIONAL)"specific carbohydrate-active enzyme classes"
Fraction-Specific CAZyme Functional Niches
NICHE_PARTITIONINGSource Taxon: Chloroflexota
Target Taxon: Thermotogota
Metabolites: cellulose (CHEBI:18246), xylan (CHEBI:37686)
Biological Processes:
- biological process involved in interspecies interaction between organisms (GO:0044419)
Evidence
-
PMID:35790765 - SUPPORT (COMPUTATIONAL)"suggesting the presence of functional niches"
-
PMID:35790765 - SUPPORT (COMPUTATIONAL)"substrate bound"
Hydrogenotrophic Methanogenic Sink
SYNTROPHYSource Taxon: Euryarchaeota
Metabolites: methane (CHEBI:16183), carbon dioxide (CHEBI:16526), dihydrogen (CHEBI:18276)
Biological Processes:
- methanogenesis (GO:0015948)
Evidence
-
PMID:35790765 - SUPPORT (IN_VITRO)"The main products observed were methane"
-
PMID:35790765 - SUPPORT (COMPUTATIONAL)"all hydrogenotrophic methanogenesis pathway enzymes"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
|
High-solids microbiome MassIVE metaproteomics dataset
Raw mass spectra for the metaproteomics study of the high-solids switchgrass microbiome. |
METAPROTEOME | OTHER | MSV000088319 |
|
High-solids microbiome ProteomeXchange dataset
ProteomeXchange record for the metaproteomics data from the high-solids switchgrass microbiome. |
METAPROTEOME | OTHER | PXD029582 |
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Exact-system primary publication - high-solids switchgrass microbiome
Primary ORNL/NREL-associated publication reporting metaproteomics of the anaerobic thermophilic switchgrass microbiome at increasing solids loading. |
OTHER | PMID:35790765 |
|
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|
Nature Communications full-text article
Open-access article with methods, data availability, and supplementary metaproteomics tables. |
OTHER | doi:10.1038/s41467-022-31433-x |
|
MassIVE dataset DOI
Dataset DOI for raw metaproteomics mass spectra. |
OTHER | doi:10.25345/C5H85T |
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Switchgrass solids loading | 30 to 150 | g/L |
|
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| Anaerobic thermophilic operation | anaerobic thermophilic enrichment | N/A |
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| High-solids enzyme response | increased AA6, beta-glucosidase, xylosidase, debranching, and pectin-acting enzyme abundance | N/A |
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