A stably transmitted 20-strain synthetic gut microbiota from honeybee intestine that primes host immunity and reduces Hafnia alvei prevalence.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| honeybee gut bacterial Core-20 members — ontology label: Bacteria | NCBITaxon:2 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Core-20 Gut Colonization
The defined Core-20 inoculum established gut communities in honeybees. During four serial passages, most input strains were detected, while G. sp. W8131 was below detection or failed to colonize. Relative abundance and diversity changed, so this is not unchanged composition or confirmed persistence of every strain. The separate passaging experiment did not establish that every passage preserved immune stimulation or pathogen protection. The canonical Bacteria participant is a broad placeholder, not a resolved 20-strain roster.
Participating Taxa: honeybee gut bacterial Core-20 members
Biological Processes:
- multi-organism process (GO:0044419 · PathwayMech)
PARTIAL - experimental Core-20 inoculation and establishment provide the microbial exposure that induces host immune-gene expression before challenge. The positive treatment response supports this link, but the separate serial-passage result does not establish persistent colonization as the necessary mediator or protection at every passage.
Evidence
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PMID:36532452 - SUPPORT (IN_VIVO)"All strains except G. sp. W8131 were detected individually in bee gut samples among passages"
Core-20 Immune Priming
Core-20 treatment increased host innate immune-gene expression: selected Toll/Imd pathway genes at 24 h and abaecin, apidaecin, hymenoptaecin and defensin-1 transcripts on Day 7 before Hafnia alvei challenge. These are positive qPCR transcriptional responses, not direct AMP protein concentration or antimicrobial activity measurements. The authors propose immune priming as part of protection; selective immune or PTS perturbations were not reported in this study.
Participating Taxa: honeybee gut bacterial Core-20 members
Biological Processes:
- regulation of innate immune response (GO:0045088 · PathwayMech)
PARTIAL - induced host immune effectors could restrict Hafnia alvei after challenge. Prechallenge immune-gene induction and subsequent reduced pathogen load support the authors' protective mechanism proposal, but no selective immune-pathway perturbation establishes mediation or excludes nutrient competition and other community effects.
Evidence
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PMID:36532452 - SUPPORT (IN_VIVO)"bees with the Core-20 showed a significant increase of AMPs abaecin, apidaecin, hymenoptaecin, and defensin-1"
Core-20-Associated Hafnia alvei Load Reduction
Core-20 treatment reduced Hafnia alvei load by the reported 78-fold relative to microbiota-free bees five days after oral challenge; load was measured by qPCR and normalized to host actin. Core-6 did not significantly reduce the load in that comparison. This positive community-treatment outcome is not pathogen eradication, direct viable-cell enumeration or demonstrated immune mediation. The strain-composition comparison does not isolate richness from strain identity, and the broad canonical bacterial participant does not resolve host, pathogen or individual symbiont identities.
Participating Taxa: honeybee gut bacterial Core-20 members
Biological Processes:
- defense response to bacterium (GO:0042742 · PathwayMech)
Evidence
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PMID:36532452 - SUPPORT (IN_VIVO)"bees treated with the Core-6, including all these strains demonstrating the ability to inhibit pathogens, did not show a significant reduction of H. alvei, while the Core-20 reduced the H. alvei loads by 78 times"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which Core-20 members and host mechanisms cause the reduced pathogen load?
Retain the positive immune-gene and load responses while testing immune-pathway necessity, peptide activity and strain-resolved contributions separately. The primary study proposes PTS-linked immune stimulation and nutrient-niche occupation from comparative genomic potential; neither is a newly demonstrated causal branch here. Four-passage detection is a separate endpoint with W8131 uncertainty, changing diversity and no protective challenge demonstrated for each passage. Existing broad Bacteria grounding is preserved rather than expanded to unverified CURIEs. Non-graph evidence classification and metadata are preserved, not recertified.
Attaches to: ecological_interactions#Core-20 Gut Colonization, ecological_interactions#Core-20 Immune Priming, ecological_interactions#Core-20-Associated Hafnia alvei Load Reduction
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| stably transmitted defined microbiota | The Core-20 community was passaged through honeybee guts and evaluated for stable colonization and immune effects. | N/A |
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Growth Media
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