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Industrial Milk-Line Four-Species Model Biofilm

Community ID CommunityMech:000152
Ecological State ENGINEERED
Environment laboratory dairy-processing biofilm model
ENVO:01001405

A laboratory four-species bacterial biofilm model derived from an industrial milk pasteurization-line biofilm. The community contains Rhodocyclus sp., Pseudomonas fluorescens, Kocuria varians, and Bacillus cereus, forms reproducible biofilms, and was used to resolve dynamic positive and negative interspecies interactions, including a Bacillus cereus thiocillin I-mediated negative interaction that shapes species distribution and robustness.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Rhodocyclus sp. NCBITaxon:1064
CROSS_FEEDER
N/A
  • PMID:34964290 - SUPPORT (IN_VITRO)
    "Rhodocyclus sp., Pseudomonas fluorescens, Kocuria varians, and Bacillus cereus"
Pseudomonas fluorescens NCBITaxon:294
CROSS_FEEDER
N/A
  • PMID:34964290 - SUPPORT (IN_VITRO)
    "Strains CLL49 and CCL56 were identified respectively as Pseudomonas fluorescens and Kocuria varians"
Kocuria varians NCBITaxon:1272
CROSS_FEEDER
N/A
  • PMID:34964290 - SUPPORT (IN_VITRO)
    "Strains CLL49 and CCL56 were identified respectively as Pseudomonas fluorescens and Kocuria varians"
Bacillus cereus NCBITaxon:1396
CROSS_FEEDER
N/A
  • PMID:34964290 - SUPPORT (IN_VITRO)
    "Bacillus cereus ATCC14579T and B. cereus ATCC10987"

Ecological Interactions

Ecological interaction network for Industrial Milk-Line Four-Species Model Biofilm Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Dynamic Positive and Negative Biofilm Interactions

NICHE_PARTITIONING

Biological Processes:

Evidence

Bacillus Thiocillin-Mediated Negative Interaction

COMPETITION

Source Taxon: Bacillus cereus

Biological Processes:

  • interspecies interaction between organisms (GO:0044419)
Downstream Effects:
→ species distribution

Evidence

  • PMID:34964290 - SUPPORT (IN_VITRO)
    "thiocillin I synthesized by the B. cereus strain"
  • PMID:34964290 - SUPPORT (IN_VITRO)
    "impact on robustness by mutational analysis"

Robustness to Transient Perturbation

COLONIZATION_FACILITATION

Biological Processes:

Evidence

Associated Datasets

Dataset Type Repository Accession
Four-species model biofilm publication and raw-data resource
Publication reporting species-resolved biofilm growth, perturbation robustness, interaction inference, and mutational analysis of a four-species dairy-processing biofilm model. 		PHENOTYPE OTHER PMID:34964290

External Resources

Name Repository Resource ID
PNDB raw data page for dynamic four-species model biofilm
Public raw-data landing page associated with the four-species model biofilm publication. 		OTHER PMID:34964290

Environmental Factors

Factor Value Unit
industrial milk pasteurization-line source biofilm from a gasket in a milk pasteurization line N/A
  • PMID:34964290 - SUPPORT (IN_VITRO)
    "derived from a biofilm isolated from an industrial milk pasteurization unit"
tryptic soy cultivation Tryptic soy agar and tryptic soy broth at 30 C N/A

Growth Media

tryptic soy broth biofilm medium

Temperature 30 C
Atmosphere AEROBIC

Preparation notes: Strains were routinely cultivated on tryptic soy agar plates or in liquid tryptic soy broth with agitation.

Evidence

doi:10.1002/mbo3.1254

"liquid tryptic soy broth (TSB)"