A defined two-isolate bacterial consortium, KZ, assembled from Klebsiella sp. CW-D3T and Arthrobacter sp. SZ-3 to degrade phenanthrene under 25 mg/L Cd2+ stress. KZ outperformed the two monocultures by 1.2-1.9-fold for phenanthrene degradation and mineralization under cadmium stress. The division of labor inferred from biomass-normalized enzyme assays assigned stronger upstream 2H1N conversion to SZ-3 and more than 80% of downstream mineralization to CW-D3T, while metagenomics associated cadmium tolerance with CW-D3T efflux-pump/antioxidant genes and the SZ-3 oxidative-stress response.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
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| Klebsiella sp. CW-D3T — ontology label: Klebsiella | NCBITaxon:570 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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DOMINANT |
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| Arthrobacter sp. SZ-3 — ontology label: Arthrobacter | NCBITaxon:1663 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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DOMINANT |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Assay-Inferred Phenanthrene Catabolic Division Of Labor
Under 25 mg/L cadmium stress, the KZ pair showed better phenanthrene degradation and mineralization than the monocultures. Biomass-normalized enzyme assays assign stronger upstream 2H1N conversion to Arthrobacter sp. SZ-3 and a dominant downstream mineralization contribution to Klebsiella sp. CW-D3T. PARTIAL - these complementary activities support a division-of-labor model, not a resolved donor-to-recipient transfer or necessary mediation. The linked corrigendum and complete assay Methods remain unverified.
Participating Taxa: Klebsiella sp. CW-D3T, Arthrobacter sp. SZ-3
Metabolites: phenanthrene (CHEBI:28851 · MediaIngredientMech · CMMMech)
Evidence
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PMID:41365368 - PARTIAL (IN_VITRO)"Through biomass-normalized enzyme activity assays, we uncovered a structured division of labor: SZ-3 exhibited superior upstream catalytic activity (50 % higher 2H1N conversion), while CW-D3T dominated downstream mineralization (>80 % contribution)."
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PMID:41365368 - SUPPORT (IN_VITRO)"which synergistically enhanced phenanthrene (PHE) degradation and mineralization under cadmium stress (25 mg/L Cd2+), outperforming monocultures by 1.2-1.9-fold."
Knowledge gaps & discussions (2)
KNOWLEDGE_GAP OPEN Which growth medium, inoculation ratio, and initial phenanthrene concentration were used to assemble and assay the KZ consortium?
The PubMed abstract identifies the two KZ members, the 25 mg/L cadmium stressor, and the phenanthrene-mineralization phenotype, but it does not expose the medium recipe, inoculum composition, or phenanthrene dose needed for a fully reproducible cultivation record.
Attaches to: engineering_design#assembly_strategy
KNOWLEDGE_GAP OPEN What does the corrigendum change, and which transfers mediate KZ performance?
The complete abstract and public publisher preview support positive KZ outcomes and assay-inferred upstream/downstream roles. Full enzyme Methods, supplements and tracer or selective perturbation evidence were not read. PMID:41456976 / doi:10.1016/j.biortech.2025.133853 is a linked corrigendum; the official publisher API supplied metadata only, not its body. Do not assume the correction is biologically immaterial or infer expression from metagenomic gene content. #1541 tracks unresolved verification. Keep this record needs_research after the bounded #1540 repair. Existing medium/inoculation questions remain open; routine LB/MSM mentioned in the preview is not a verified co-contamination assay recipe.
Attaches to: ecological_interactions#Assay-Inferred Phenanthrene Catabolic Division Of Labor
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Primary publication - Klebsiella-Arthrobacter KZ phenanthrene-Cd2+ SynCom
Bioresource Technology 2026 primary article describing the KZ Klebsiella-Arthrobacter consortium for phenanthrene mineralization under cadmium stress. |
OTHER | PMID:41365368 |
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Environmental Factors
| Factor | Value | Unit |
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| Cadmium co-contamination stress | 25 | mg/L Cd2+ |
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Growth Media
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