A defined two-member engineered yeast-bacterium consortium pairing Komagataella phaffii with Escherichia coli for co-inducible relayed biosynthesis of natural products. Low-level glucose-responsive and blue-light-responsive transcriptional systems were rebuilt separately for the two hosts so a single signal could co-induce compound synthesis in coculture. After pathway redirection, genome mining, and rewiring of acyl-donor degradation targets, the living consortium produced the reporter molecule simvastatin from a simple carbon source and also supported inducible biosynthesis of (2S)-naringenin.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Komagataella phaffii | NCBITaxon:460519 · TaxonMech · TraitMech | N/A | |
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| Escherichia coli | NCBITaxon:562 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
ℹ️ No detailed ecological interaction data is available for this community.
This record may be a metabolic model or minimal entry containing only taxonomy and external resource links.
Knowledge gaps & discussions (2)
KNOWLEDGE_GAP OPEN Which biosynthetic intermediate or intermediates pass between K. phaffii and E. coli during simvastatin and naringenin production?
The abstract reports relayed synthesis and single-signal co-induction in coculture, but the accessible cache does not identify the exchanged metabolite or assign each reporter pathway segment to K. phaffii versus E. coli.
Attaches to: engineering_design#assembly_strategy
KNOWLEDGE_GAP OPEN Which strain-resolved relays and induction responses support a causal biosynthesis graph?
The abstract and public publisher Introduction/section previews report compatible co-growth, common-signal induction and relayed simvastatin/naringenin production. A design without engineered population-balancing interactions does not prove biological neutralism; sharing an induction signal is not metabolite cross-feeding. Monacolin J acid and an acyl donor are discussed, but exact chassis/construct assignments, transfer controls and full Methods remain unresolved. #1542 tracks the empty graph as needs_research, not absent biology. A reviewed one-community LITERATURE dry run contains 7,457 characters; zero provider submissions or credits. Specifically authorized research and primary verification are needed before adding new nodes/arrows. Preserve the existing intermediate-assignment discussion and canonical species IDs.
Attaches to: ecological_interactions, engineering_design
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Primary publication - K. phaffii-E. coli co-inducible biosynthesis coculture
Metabolic Engineering 2026 primary article describing the Komagataella phaffii and Escherichia coli consortium for co-inducible relayed synthesis of simvastatin and (2S)-naringenin. |
OTHER | PMID:42492893 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Glucose and blue-light induction | low-level glucose- and blue light-responsive transcriptional systems | N/A |
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Growth Media
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