Kombucha KMC-IMBG1 Fermentation Community

Community ID CommunityMech:000154

Ecological State STABLE

Environment laboratory kombucha fermentation culture
ENVO:01001405

A Ukrainian kombucha microbial community variant, KMC-IMBG1, profiled by culture-dependent isolation and 16S/ITS metabarcoding across sweetened black-tea and honey/cabbage-brine microenvironments. The core community contains cellulose-forming acetobacteria, especially Komagataeibacter and Gluconobacter, together with dominant yeasts such as Pichia and Brettanomyces/Dekkera; growth conditions alter the accessory community and yeast composition.

Taxonomy

Taxon	Ontology ID	Functional Roles	Abundance
Komagataeibacter	NCBITaxon:1434011	PRIMARY_DEGRADER	DOMINANT
PMID:26061774 - SUPPORT (IN_VITRO) "acetobacteria of two genera Komagataeibacter (former Gluconacetobacter) and Gluconobacter"
Gluconobacter	NCBITaxon:441	PRIMARY_DEGRADER	ABUNDANT
doi:10.1186/s13568-015-0124-5 - SUPPORT (IN_VITRO) "acetobacteria of two genera Komagataeibacter (former Gluconacetobacter) and Gluconobacter"
Pichia	NCBITaxon:4919	SECONDARY_FERMENTER	DOMINANT
PMID:26061774 - SUPPORT (IN_VITRO) "Brettanomyces/Dekkera and Pichia (including former Issatchenkia) were dominant"
Brettanomyces	NCBITaxon:13366	SECONDARY_FERMENTER	DOMINANT
doi:10.1186/s13568-015-0124-5 - SUPPORT (IN_VITRO) "Brettanomyces/Dekkera and Pichia (including former Issatchenkia) were dominant"

Ecological Interactions

Taxon

Cross-feeding

Mutualism

Syntrophy

Competition

Commensalism

Niche partitioning

Colonization facilitation

Strain competition

Predation

Yeast-Acetobacter Mutualistic Fermentation

MUTUALISM

Biological Processes:

fermentation (GO:0006113)
biological process involved in interspecies interaction between organisms (GO:0044419)

Evidence

PMID:26061774 - SUPPORT (IN_VITRO)

"mutualistic metabolic cooperation of pro- and eukaryotic microorganisms"

Cellulose Pellicle Community Structuring

COLONIZATION_FACILITATION

Source Taxon: Komagataeibacter

Biological Processes:

biofilm formation (GO:0042710)

Evidence

doi:10.1186/s13568-015-0124-5 - SUPPORT (IN_VITRO)

"cellulose-based pellicles created by the cellulose-producing bacteria"

Growth-Condition Dependent Accessory Recruitment

NICHE_PARTITIONING

Biological Processes:

biological process involved in interspecies interaction between organisms (GO:0044419)

Evidence

PMID:26061774 - SUPPORT (IN_VITRO)

"The community composition was dependent on the growth conditions"
PMID:26061774 - SUPPORT (IN_VITRO)

"bacterial core component in nsBTS remained the same"

Associated Datasets

Dataset	Type	Repository	Accession
KMC-IMBG1 metabarcoding and isolate sequence data Article reports 16S rDNA and ITS metabarcoding of KMC-IMBG1 pellicles and liquids across multiple growth microenvironments, plus GenBank accessions for isolate 16S rDNA sequences.	AMPLICON_16S	OTHER	KF908872-KF908879

Environmental Factors

Factor	Value	Unit
sweetened black tea substrate	black tea extract with sucrose	N/A
PMID:26061774 - SUPPORT (IN_VITRO) "black tea (Lipton, 1.2%, w/v) extract with sucrose (3.0%, w/v)"
cultivation without shaking	14 days at 28 C without shaking	N/A
doi:10.1186/s13568-015-0124-5 - SUPPORT (IN_VITRO) "cultivation for 14 days at 28°C without shaking"

Growth Media

sterile black tea with sucrose

Temperature 28 C

Composition

Component	Concentration	Unit	CHEBI
sucrose	3.0	percent w/v	CHEBI:17992

Preparation notes: Filter-sterilized black tea extract with sucrose; KMC-IMBG1 was also maintained in nonsterile sweetened black tea and sterile black tea with honey.

Evidence

PMID:26061774

"maintained in a filter sterilized black tea"

PMID:26061774

"A matured KMC-IMBG1 was obtained after cultivation for 14 days at 28°C without shaking"