MUC2 Human Gut Commensal Defined Consortium

After 72 hours, the porcine-MUC2 treatment increased the Akkermansia qPCR estimate and decreased the Prevotella estimate relative to vehicle controls. Six qPCR groups produced calculated CFU equivalents from DNA standard curves, not direct viable counts or resolution of all fourteen inoculated strains. Total OD did not significantly differ between treatments. The fourteen canonical participants identify the input roster, not confirmed equal survival or endpoint abundance. CAZy glycosyl-hydrolase capacity classes summarize species-level genome sets, not measured mucin-degrading enzymes in these exact inoculants; niche partitioning and antagonism are not established by these endpoints.

Participating Taxa: Lactobacillus acidophilus ATCC 4796, Lactococcus lactis CB1, Clostridium symbiosum DSZM 14940, Streptococcus thermophilus ATCC 491, Escherichia coli Nissle 1917, Enterococcus faecalis Symbioflor DSM 16431, Bifidobacterium longum ATCC 55813, Blautia coccoides ATCC 29236, Blautia producta ATCC 27340D, Prevotella copri DSM 18205, Bacteroides thetaiotaomicron ATCC 29148, Bacteroides fragilis ATCC 23745, Bacteroides ovatus ATCC 8483, Akkermansia muciniphila ATCC BAA-83

Evidence

• PMID:42007376 - SUPPORT (IN_VITRO)
  "MUC2 supplementation significantly altered community structure, promoting the expansion of Akkermansia muciniphila while reducing Prevotella."

At 72 hours, MUC2-supplemented bioreactors had lower acetate and higher propionate, butyrate and formate concentrations in filtered cell-free supernatants than vehicle controls, measured by targeted LC-MS/MS. These are net extracellular concentration endpoints, not production rates, traced mucin-carbon flux or donor-recipient exchange. The experiment used three bioreactors per treatment, 0.5 mg/mL porcine MUC2 and daily 10% medium replacement; pH was neither controlled nor measured. HYPOTHESIZED - mucin-derived substrates and cross-feeding may contribute, but specific producers, consumers and composition-mediated effects were not established. The fourteen listed participants are input members, not assigned acid producers, and no host response was tested.

Participating Taxa: Lactobacillus acidophilus ATCC 4796, Lactococcus lactis CB1, Clostridium symbiosum DSZM 14940, Streptococcus thermophilus ATCC 491, Escherichia coli Nissle 1917, Enterococcus faecalis Symbioflor DSM 16431, Bifidobacterium longum ATCC 55813, Blautia coccoides ATCC 29236, Blautia producta ATCC 27340D, Prevotella copri DSM 18205, Bacteroides thetaiotaomicron ATCC 29148, Bacteroides fragilis ATCC 23745, Bacteroides ovatus ATCC 8483, Akkermansia muciniphila ATCC BAA-83

Metabolites: acetate (CHEBI:30089 · MediaIngredientMech · CMMMech), propionate (CHEBI:17272 · MediaIngredientMech · CMMMech), butyrate (CHEBI:17968 · MediaIngredientMech · CMMMech), formate (CHEBI:15740 · MediaIngredientMech · CMMMech)

Evidence

• PMID:42007376 - SUPPORT (IN_VITRO)
  "MUC2 also reshaped microbial metabolism, decreasing acetate levels while increasing propionate, butyrate, and formate."