MUCC Freshwater Wetland Methane-Cycling Network Community

Community ID CommunityMech:000257

Ecological State STABLE

Environment terrestrial freshwater wetland
ENVO:00000044

A multi-site terrestrial freshwater wetland microbial community synthesis curated around the Multi-Omics for Understanding Climate Change (MUCC) v2.0.0 database. The study integrates 16S rRNA amplicon datasets, metagenomes, metatranscriptomes, and annual methane flux measurements across nine wetlands to link microbial community structure, methane-cycling guilds, decomposition networks, and methane emissions. It identifies eight methane-cycling genera shared across wetlands, highlights wetland-specific metabolic interactions in marshes, and reports Methanoregula as a hub methanogen and predictor of methane flux. The record is DOE-relevant because the study was supported by DOE Office of Science BER awards and included metagenomic and metatranscriptomic sequencing at the DOE Joint Genome Institute.

Taxonomy

Taxon	Ontology ID	Functional Roles	Abundance
MUCC freshwater wetland microbial community	NCBITaxon:131567	PRIMARY_DEGRADER SECONDARY_FERMENTER SYNTROPHIC_PARTNER	N/A
PMID:39843444 - SUPPORT (COMPUTATIONAL) "integrate 16S rRNA amplicon datasets, metagenomes, metatranscriptomes, and annual methane flux data across 9 wetlands" PMID:39843444 - SUPPORT (COMPUTATIONAL) "creating the Multi-Omics for Understanding Climate Change (MUCC) v2.0.0 database"
MUCC wetland methanogens	NCBITaxon:2157	SYNTROPHIC_PARTNER PRIMARY_PRODUCER	COMMON
PMID:39843444 - SUPPORT (COMPUTATIONAL) "methane-cycling microbes and the networks driving carbon decomposition" PMID:39843444 - SUPPORT (COMPUTATIONAL) "methanogens and methanotrophs in high-emitting wetlands"
Methanoregula	NCBITaxon:395331	SYNTROPHIC_PARTNER PRIMARY_PRODUCER	COMMON
PMID:39843444 - SUPPORT (COMPUTATIONAL) "Methanoregula emerged as a hub methanogen across networks" PMID:39843444 - SUPPORT (COMPUTATIONAL) "strong predictor of methane flux"
MUCC wetland methanotrophs	NCBITaxon:2	PRIMARY_DEGRADER	COMMON
PMID:39843444 - SUPPORT (COMPUTATIONAL) "identify eight methane-cycling genera shared across wetlands" PMID:39843444 - SUPPORT (COMPUTATIONAL) "low connections between methanogens and methanotrophs in high-emitting wetlands"

Ecological Interactions

Taxon

Cross-feeding

Mutualism

Syntrophy

Competition

Commensalism

Niche partitioning

Colonization facilitation

Strain competition

Predation

Conserved Methane-Cycling Genera Across Wetlands

NICHE_PARTITIONING

Metabolites: methane (CHEBI:16183)

Biological Processes:

methanogenesis (GO:0015948)
methane metabolic process (GO:0015947)

Evidence

PMID:39843444 - SUPPORT (COMPUTATIONAL)

"identify eight methane-cycling genera shared across wetlands"
PMID:39843444 - SUPPORT (COMPUTATIONAL)

"Microorganisms drive methane cycling"

Marsh Metabolic Interaction Networks

NICHE_PARTITIONING

Source Taxon: MUCC wetland methanogens

Target Taxon: MUCC wetland methanotrophs

Metabolites: methane (CHEBI:16183)

Biological Processes:

methane metabolic process (GO:0015947)

Evidence

PMID:39843444 - SUPPORT (COMPUTATIONAL)

"wetland-specific metabolic interactions in marshes"
PMID:39843444 - SUPPORT (COMPUTATIONAL)

"low connections between methanogens and methanotrophs in high-emitting wetlands"

Methanoregula Hub Methanogen And Flux Predictor

SYNTROPHY

Source Taxon: Methanoregula

Metabolites: methane (CHEBI:16183)

Biological Processes:

methanogenesis (GO:0015948)

Evidence

PMID:39843444 - SUPPORT (COMPUTATIONAL)

"Methanoregula emerged as a hub methanogen across networks"
PMID:39843444 - SUPPORT (COMPUTATIONAL)

"strong predictor of methane flux"

Methylotrophic Methanogenesis Potential

NICHE_PARTITIONING

Source Taxon: Methanoregula

Metabolites: methane (CHEBI:16183), methanol (CHEBI:17790)

Biological Processes:

methanogenesis (GO:0015948)

Evidence

PMID:39843444 - SUPPORT (COMPUTATIONAL)

"functional potential for methylotrophic methanogenesis"
PMID:39843444 - SUPPORT (COMPUTATIONAL)

"highlighting the importance of this pathway"

Associated Datasets

Dataset	Type	Repository	Accession
MUCC v2.0.0 Zenodo database Zenodo archive for MUCC v2.0.0 processed MAGs, 16S rRNA tables, metatranscriptomic abundance tables, DRAM annotations, and analysis files.	OTHER	ZENODO	10.5281/zenodo.14532347
MUCC BioProject PRJNA330672 NCBI BioProject listed by the MUCC v2.0.0 Nature Communications data availability statement.	OTHER	NCBI_BIOPROJECT	PRJNA330672
MUCC BioProject PRJNA1007388 NCBI BioProject listed by the MUCC v2.0.0 Nature Communications data availability statement.	OTHER	NCBI_BIOPROJECT	PRJNA1007388
MUCC BioProject PRJNA216952 NCBI BioProject listed by the MUCC v2.0.0 Nature Communications data availability statement.	OTHER	NCBI_BIOPROJECT	PRJNA216952
MUCC BioProject PRJNA638786 NCBI BioProject listed by the MUCC v2.0.0 Nature Communications data availability statement.	OTHER	NCBI_BIOPROJECT	PRJNA638786

External Resources

Name	Repository	Resource ID
Primary publication for MUCC freshwater wetland methane networks PubMed record for the Nature Communications paper describing MUCC v2.0.0 and metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands.	OTHER	PMID:39843444
PMID:39843444 - SUPPORT (OTHER) "Metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands"
DOI landing page DOI for the Nature Communications publication.	OTHER	doi:10.1038/s41467-025-56133-0
PMID:39843444 - SUPPORT (OTHER) "DOI: 10.1038/s41467-025-56133-0"
USGS Publications Warehouse record USGS Publications Warehouse record for the Nature Communications MUCC v2.0.0 wetland methane-cycling article.	OTHER	USGS:70268258

Environmental Factors

Factor	Value	Unit
Wetland type	marsh, swamp, fen, and bog	N/A
PMID:39843444 - SUPPORT (COMPUTATIONAL) "across 9 wetlands"
Annual methane flux	annual methane flux data	N/A
PMID:39843444 - SUPPORT (IN_VIVO) "annual methane flux data"
High methane-emitting wetlands	high methane emission context	N/A
PMID:39843444 - SUPPORT (COMPUTATIONAL) "high methane-emitting wetlands"