A defined artificial aerobic coculture pairing the marine methanotroph Methylocaldum marinum S8 with the non-methylotrophic acetate-utilizing bacterium Cupriavidus necator NBRC 102504. The coculture was used to test whether methane-derived metabolites excreted by M. marinum S8 can support a partner that is not a methylotroph. The system is DOE-relevant because it links methane oxidation to acetate-mediated growth of a bioproduction-relevant heterotroph, providing a model for methane-to-bioproduct community design.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Methylocaldum marinum S8 | NCBITaxon:1432792 |
PRIMARY_DEGRADER
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N/A |
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| Cupriavidus necator NBRC 102504 | NCBITaxon:1218105 |
CROSS_FEEDER
SECONDARY_FERMENTER
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COMMON |
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Ecological Interactions
Methane-Derived Acetate Cross-Feeding To Cupriavidus
CROSS_FEEDINGSource Taxon: Methylocaldum marinum S8
Target Taxon: Cupriavidus necator NBRC 102504
Metabolites: methane (CHEBI:16183), acetate (CHEBI:30089)
Biological Processes:
- methane metabolic process (GO:0015947)
- acetate metabolic process (GO:0006083)
Evidence
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doi:10.1093/bbb/zbab150 - SUPPORT (IN_VITRO)"supporting the existence of methanol-independent cross-feeding from M. marinum S8"
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doi:10.1093/bbb/zbab150 - SUPPORT (IN_VITRO)"possibly utilizes acetate rather than methanol"
Oxygen-Dependent Acetate Excretion Window
COLONIZATION_FACILITATIONSource Taxon: Methylocaldum marinum S8
Target Taxon: Cupriavidus necator NBRC 102504
Metabolites: oxygen (CHEBI:15379), acetate (CHEBI:30089)
Biological Processes:
- methane metabolic process (GO:0015947)
Evidence
-
doi:10.1093/bbb/zbab150 - SUPPORT (IN_VITRO)"not only under microaerobic"
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doi:10.1093/bbb/zbab150 - SUPPORT (IN_VITRO)"but also under aerobic"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
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Methylocaldum marinum S8 genome assembly
NCBI genome assembly for the M. marinum S8 methanotroph. |
GENOME | OTHER | GCA_003584645.1 |
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Cupriavidus necator NBRC 102504 genome assembly
NCBI genome assembly for the C. necator NBRC 102504 partner. |
GENOME | OTHER | GCA_001598755.1 |
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Exact-system primary publication - Methylocaldum/Cupriavidus cross-feeding
Primary article reporting acetate excretion by M. marinum S8 and growth of C. necator NBRC 102504 in the M. marinum S8 coculture. |
OTHER | doi:10.1093/bbb/zbab150 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Methanotrophic aerobic context | methane-oxidizing aerobic coculture | N/A |
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| Oxygen concentration regimes | 3.5-6 and 20-31 | percent O2 |
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