A defined aerobic methane-fed laboratory coculture pairing the marine methanotroph Methylocaldum marinum S8 with the facultative methylotroph Methyloceanibacter caenitepidi Gela4. M. marinum S8 oxidizes methane and supports syntrophic growth of M. caenitepidi Gela4 on methane-derived carbon. Transcriptome and chemical analyses indicate that acetate, rather than methanol alone, is likely a major cross-fed carbon source in this model methanotroph-methylotroph community.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Methylocaldum marinum S8 | NCBITaxon:1432792 |
PRIMARY_DEGRADER
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N/A |
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| Methyloceanibacter caenitepidi Gela4 | NCBITaxon:1384459 |
CROSS_FEEDER
SECONDARY_FERMENTER
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N/A |
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Ecological Interactions
Methane-Derived Carbon Cross-Feeding
CROSS_FEEDINGSource Taxon: Methylocaldum marinum S8
Target Taxon: Methyloceanibacter caenitepidi Gela4
Metabolites: methane (CHEBI:16183), acetate (CHEBI:30089)
Biological Processes:
- methane metabolic process (GO:0015947)
- acetate metabolic process (GO:0006083)
Evidence
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PMID:30870453 - SUPPORT (IN_VITRO)"cross-feeding on methane-derived carbon"
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PMID:30870453 - SUPPORT (IN_VITRO)"organic compound other than methanol, possibly acetate"
Methanol-Independent Methylotroph Response
CROSS_FEEDINGSource Taxon: Methylocaldum marinum S8
Target Taxon: Methyloceanibacter caenitepidi Gela4
Metabolites: methanol (CHEBI:17790), acetate (CHEBI:30089)
Biological Processes:
- methanol metabolic process (GO:0015945)
- acetate metabolic process (GO:0006083)
Evidence
-
PMID:30870453 - SUPPORT (IN_VITRO)"methanol was below the detection limit"
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PMID:30870453 - SUPPORT (IN_VITRO)"acetyl-CoA synthetase, were upregulated"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
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M. caenitepidi Gela4 complete genome
BioProject for the complete genome of the Gela4 methylotrophic partner used in the coculture. |
GENOME | NCBI_BIOPROJECT | PRJDB3104 |
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M. caenitepidi Gela4 and M. marinum S8 RNA-seq dataset
DDBJ Sequence Read Archive RNA-seq dataset comparing Gela4 pure culture and methane-fed Gela4/S8 coculture conditions. |
METATRANSCRIPTOME | OTHER | DRA006072 |
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Exact-system publication - Methylocaldum/Methyloceanibacter cross-feeding
Primary publication reporting the exact M. marinum S8 and M. caenitepidi Gela4 methane-fed coculture, RNA-seq response, and likely acetate-mediated cross-feeding. |
OTHER | PMID:30870453 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Methane and oxygen availability | methane/air headspace, 20:80 v/v | N/A |
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