A defined synthetic methanotroph-cyanobacterium coculture developed at Pacific Northwest National Laboratory for simultaneous use of methane and carbon dioxide gas feedstocks. The methanotrophic bacterium Methylomicrobium alcaliphilum 20z, currently represented by NCBI as Methylotuvimicrobium alcaliphilum 20Z, is coupled to the cyanobacterium Synechococcus PCC 7002, currently represented by NCBI as Picosynechococcus sp. PCC 7002. The coculture converts CH4 and CO2 into microbial biomass through oxygenic photosynthesis-driven O2 supply to the methanotroph and respiratory CO2 supply to the photoautotroph, with follow-up work using the same species pair for quantitative coculture characterization.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Methylomicrobium alcaliphilum 20z | NCBITaxon:1091494 |
PRIMARY_DEGRADER
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ABUNDANT |
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| Synechococcus PCC 7002 | NCBITaxon:32049 |
PRIMARY_PRODUCER
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ABUNDANT |
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Ecological Interactions
Photosynthetic O2 Support for Methane Oxidation
MUTUALISMSource Taxon: Synechococcus PCC 7002
Target Taxon: Methylomicrobium alcaliphilum 20z
Metabolites: oxygen (CHEBI:15379), methane (CHEBI:16183)
Biological Processes:
- photosynthesis (GO:0015979)
- methane metabolic process (GO:0015947)
Evidence
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PMID:28092828 - SUPPORT (IN_VITRO)"via oxygenic photosynthesis"
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PMID:28092828 - SUPPORT (IN_VITRO)"does not require CH4/O2 mixtures to be fed"
CO2 Utilization by Cyanobacterial Partner
CROSS_FEEDINGSource Taxon: Methylomicrobium alcaliphilum 20z
Target Taxon: Synechococcus PCC 7002
Metabolites: carbon dioxide (CHEBI:16526)
Biological Processes:
- carbon fixation (GO:0015977)
- photosynthesis (GO:0015979)
Evidence
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PMID:28092828 - SUPPORT (IN_VITRO)"simultaneous utilization of methane and carbon dioxide"
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PMID:33064298 - SUPPORT (IN_VITRO)"individual consumption and production rates of O2 and CO2"
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Exact-system primary publication - gas feedstock coculture
Primary publication reporting the M. alcaliphilum 20z and Synechococcus PCC 7002 coculture for simultaneous methane and carbon dioxide utilization. |
OTHER | PMID:28092828 |
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Exact species-pair follow-up publication - quantitative characterization
Follow-up publication using the same Methylomicrobium alcaliphilum and Synechococcus PCC 7002 species composition, with a 20ZR methanotroph derivative, for quantitative measurement of individual biomass and O2/CO2 rates. |
OTHER | PMID:33064298 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Gas feedstock composition | diverse gas mixtures ranging from biogas to natural-gas feedstock analogs | N/A |
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| Light and O2 limitation | coupled light and O2 limitations | N/A |
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