A four-member artificial soil-organic-matter microcosm inoculum consisting of Curtobacterium sp. PTCC 1316, Trichoderma harzianum IBRC-M30059, Phanerochaete chrysosporium IBRC-M30144, and Pseudomonas fluorescens IBRC-M10752. Maqami et al. 2026 used the synthetic fungi-plus-bacteria consortium as an experimentally simplified alternative to a native agricultural-soil microbiome in 500 mL glass-jar microcosms containing sterilized bentonite or palygorskite clay, calcium carbonate, quartz sand, and wheat-straw or alfalfa-shoot litter, then quantified respiration kinetics and microbial biomass carbon over a 90-day incubation.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Curtobacterium sp. PTCC 1316 — ontology label: Curtobacterium | NCBITaxon:2034 · TaxonMech · TraitMech | COMMON | |
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| Trichoderma harzianum IBRC-M30059 — ontology label: Trichoderma harzianum | NCBITaxon:5544 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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COMMON |
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| Phanerochaete chrysosporium IBRC-M30144 — ontology label: Phanerodontia chrysosporium | NCBITaxon:2822231 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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COMMON |
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| Pseudomonas fluorescens IBRC-M10752 — ontology label: Pseudomonas fluorescens | NCBITaxon:294 · TaxonMech · TraitMech | COMMON | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Mineral-litter SOM turnover by the fungal-bacterial inoculum
The synthetic Curtobacterium / Trichoderma / Phanerochaete / Pseudomonas inoculum was assayed for net wheat- and alfalfa-litter turnover in clay-CaCO3-quartz microcosms, where total CO2 efflux and microbial biomass carbon were shaped by clay mineralogy, calcium carbonate level, litter chemistry, and comparison to native-soil inoculum.
Participating Taxa: Curtobacterium sp. PTCC 1316, Trichoderma harzianum IBRC-M30059, Phanerochaete chrysosporium IBRC-M30144, Pseudomonas fluorescens IBRC-M10752
Evidence
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PMID:42218210 - SUPPORT (IN_VITRO)"native communities generated higher cumulative C loss but lower rate constants, while the synthetic consortium drove faster mineralization and higher biomass on palygorskite."
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PMID:42218210 - SUPPORT (IN_VITRO)"In contrast, mixed fungal and bacterial inocula generally supported higher MBC in both litter types."
Knowledge gaps & discussions (2)
KNOWLEDGE_GAP OPEN What fraction of CaCO3-amended CO2 efflux was microbial respiration rather than abiotic carbonate-derived CO2?
The study measured total CO2 efflux but explicitly reports that abiotic CO2 release from CaCO3 was not separated, so carbonate-associated respiration endpoints are curated as net microcosm outcomes.
Attaches to: ecological_interactions#Mineral-litter SOM turnover by the fungal-bacterial inoculum, environmental_factors#Calcium carbonate content
KNOWLEDGE_GAP OPEN Which enzyme-sorption or molecular-scale organo-mineral stabilization pathways drove the palygorskite and CaCO3 effects?
The authors interpret respiration and biomass patterns through clay microhabitat engineering, enzyme retention, and stabilization hypotheses, but state that the underlying enzyme-sorption and molecular-scale stabilization pathways were not directly quantified.
Attaches to: ecological_interactions#Mineral-litter SOM turnover by the fungal-bacterial inoculum
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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PubMed record for the mineral microhabitat SOM SynCom article
PubMed record for the 2026 Scientific Reports clay-mineralogy artificial SOM microcosm article. |
OTHER | PMID:42218210 |
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DOI for the mineral microhabitat SOM SynCom article
Publisher DOI for the primary Scientific Reports article. |
OTHER | doi:10.1038/s41598-026-49740-4 |
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PubMed Central full text for the mineral microhabitat SOM SynCom article
Open-access PubMed Central full text used for method and result snippets. |
OTHER | PMCID:PMC13458411 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Clay mineral type and content | bentonite or palygorskite at 0, 10, or 20 | % w/w |
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| Calcium carbonate content | 7.5 or 15 | % w/w |
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| Litter quality | wheat straw or alfalfa shoots | plant litter type |
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| Controlled incubation | 22 | °C |
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Growth Media
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