A ten-strain Miscanthus root-derived SynCom with nitrification and denitrification capabilities for ammonia nitrogen removal in rare earth mine tailings.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
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| Burkholderia sp. — ontology label: Burkholderia | NCBITaxon:32008 · TaxonMech · TraitMech | N/A | |
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| Paraburkholderia sp. — ontology label: Paraburkholderia | NCBITaxon:1822464 · TaxonMech · TraitMech | N/A | |
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| Curtobacterium sp. — ontology label: Curtobacterium | NCBITaxon:2034 · TaxonMech · TraitMech | N/A | |
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| Leifsonia sp. — ontology label: Leifsonia | NCBITaxon:110932 · TaxonMech · TraitMech | N/A | |
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| Sinomonas sp. — ontology label: Sinomonas atrocyanea | NCBITaxon:37927 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Nitrogen Transformation in REE Tailings
The ten-strain root-derived SynCom was selected for nitrification and denitrification capabilities and plant growth promotion in ammonia-nitrogen-polluted REE tailings. The five named participants are the existing grouped taxonomy entries, not five strains or ten fully resolved strain identities. Capability and community-level outcomes do not establish every member contribution or reciprocal microbial fitness benefit.
Participating Taxa: Burkholderia sp., Paraburkholderia sp., Curtobacterium sp., Leifsonia sp., Sinomonas sp.
Metabolites: ammonia (CHEBI:16134 · MediaIngredientMech · CMMMech)
Biological Processes:
- nitrification (GO:0019329 · PathwayMech)
- denitrification (GO:0019333 · PathwayMech)
PARTIAL - The SynCom nitrogen-cycle capabilities support biological transformation as a contributor to the observed ammonia-nitrogen reduction. The primary abstract attributes removal jointly to microbial transformation and plant-associated uptake; the available evidence does not partition their quantitative contributions or establish every participating strain as necessary.
Evidence
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PMID:39481489 - SUPPORT (IN_VITRO)"This SynCom, consisting of 10 bacterial strains, included species of the genera Burkholderia (5) Paraburkholderia (1), Curtobacterium (1), Leifsonia (1) and Sinomonas (2)."
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PMID:39481489 - SUPPORT (IN_VITRO)"possessing nitrification and denitrification capabilities, to help REEs mine plants efficiently convert pollutant AN into nutrients"
Live-SynCom Ammonia Nitrogen Removal
The SynCom alone reduced tailing-soil ammonia nitrogen by 24.8%; with Miscanthus the reduction was 32.6%, compared with 25.5% for plants alone. These positive community/plant treatment effects are retained without calling ammonia-nitrogen disappearance a measured complete nitrogen balance, universal strain cooperation or reciprocal fitness gain.
Participating Taxa: Burkholderia sp., Paraburkholderia sp., Curtobacterium sp., Leifsonia sp., Sinomonas sp.
Metabolites: ammonia (CHEBI:16134 · MediaIngredientMech · CMMMech), ammonium (CHEBI:28938 · MediaIngredientMech · CMMMech)
Biological Processes:
- nitrification (GO:0019329 · PathwayMech)
- denitrification (GO:0019333 · PathwayMech)
HYPOTHESIZED - Reduced ammonia-nitrogen stress and altered nitrogen availability could contribute to the shoot/root biomass increase after live SynCom inoculation. The primary abstract reports both outcomes but does not isolate this mediation from other growth-promoting activities or the reverse contribution of larger plants to nitrogen uptake; this arrow is not a selectively demonstrated causal direction.
Evidence
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PMID:39481489 - SUPPORT (IN_VITRO)"this SynCom alone achieved a significant reduction of 24.8% in AN content in tailing soil"
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PMID:39481489 - SUPPORT (IN_VITRO)"When the SynCom inoculated with plants, the reduction in AN was even more significant (32.6%)"
Miscanthus Biomass Stimulation
Live SynCom inoculation increased Miscanthus shoot and root biomass by 39.8% and 49.7%, respectively, relative to dead SynCom inoculation. Seven nitrogen-cycle strains were particularly enriched, consistent with selective root association. These results establish positive inoculation effects and enrichment, not proof that enrichment itself causes biomass gain or a quantitative measurement of plant nitrogen uptake mediated by each strain.
Participating Taxa: Burkholderia sp., Paraburkholderia sp., Curtobacterium sp., Leifsonia sp., Sinomonas sp.
Metabolites: ammonia (CHEBI:16134 · MediaIngredientMech · CMMMech), ammonium (CHEBI:28938 · MediaIngredientMech · CMMMech)
Biological Processes:
- nitrification (GO:0019329 · PathwayMech)
- denitrification (GO:0019333 · PathwayMech)
Evidence
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PMID:39481489 - SUPPORT (IN_VIVO)"live SynCom inoculation significantly increased shoot and root biomass by 39.8% and 49.7%, respectively"
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PMID:39481489 - SUPPORT (IN_VIVO)"seven nitrifying and denitrifying strains of the SynCom are particularly enriched"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which nitrogen transformations and plant-growth pathways mediate the live-inoculum effects?
PMID39481489 supports positive AN removal, live-versus-dead biomass gains and enrichment of seven strains. The available primary abstract does not resolve transformation versus uptake contributions or the direction of mediation between AN loss and biomass. Full Methods, supplements and nitrogen-fate controls need inspection; abstract-only access is not evidence those controls were absent. The canonical Sinomonas sp./species-level taxon mismatch is preserved for separate metadata review, and grouped participants do not claim resolved strain-specific activity.
Attaches to: ecological_interactions#Nitrogen Transformation in REE Tailings, ecological_interactions#Live-SynCom Ammonia Nitrogen Removal, ecological_interactions#Miscanthus Biomass Stimulation
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| defined synthetic community design | Ten bacterial strains from Miscanthus floridulus roots representing Burkholderia, Paraburkholderia, Curtobacterium, Leifsonia, and Sinomonas. | N/A |
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