A six-strain psychrotolerant composite microbial agent for low-temperature nitrogen removal, generated by coculturing nitrifying consortium NC1 (Pseudomonas veronii HN1, Pseudomonas poae HN2, and Pseudomonas peli HN3) with aerobic-denitrifying consortium DC1 (Aeromonas sp. AD1, Pseudomonas extremaustralis AD2, and Serratia liquefaciens AD3) at a 1:1 inoculation ratio.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
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| Pseudomonas veronii HN1 — ontology label: Pseudomonas veronii | NCBITaxon:76761 · TaxonMech · TraitMech | DOMINANT | |
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| Pseudomonas poae HN2 — ontology label: Pseudomonas poae | NCBITaxon:200451 · TaxonMech · TraitMech | DOMINANT | |
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| Pseudomonas peli HN3 — ontology label: Pseudomonas peli | NCBITaxon:592361 · TaxonMech · TraitMech | DOMINANT | |
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| Aeromonas sp. AD1 — ontology label: Aeromonas | NCBITaxon:642 · TaxonMech · TraitMech | DOMINANT | |
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| Pseudomonas extremaustralis AD2 — ontology label: Pseudomonas extremaustralis | NCBITaxon:359110 · TaxonMech · TraitMech | DOMINANT | |
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| Serratia liquefaciens AD3 — ontology label: Serratia liquefaciens | NCBITaxon:614 · TaxonMech · TraitMech | DOMINANT | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
NC1-DC1 synergistic nitrogen removal
The complete NDC-6 community combines NC1 nitrifying strains with DC1 aerobic-denitrifying strains and outperforms the individual strains or single-function consortia in low-temperature nitrogen and COD removal.
Participating Taxa: Pseudomonas veronii HN1, Pseudomonas poae HN2, Pseudomonas peli HN3, Aeromonas sp. AD1, Pseudomonas extremaustralis AD2, Serratia liquefaciens AD3
Metabolites: ammonium (CHEBI:28938 · MediaIngredientMech · CMMMech), nitrate (CHEBI:17632 · MediaIngredientMech · CMMMech)
Biological Processes:
- ammonia oxidation (GO:0019329 · PathwayMech)
- denitrification (GO:0019333 · PathwayMech)
Evidence
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PMID:41504900 - SUPPORT (IN_VITRO)"After 3 days of incubation at 10 °C, NDC-6 achieved removal efficiencies of 89.3%, 88.1%, 85.5%, and 95.3% for NH4+-N, NO3--N, total nitrogen (TN), and chemical oxygen demand (COD)"
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PMID:41504900 - SUPPORT (IN_VITRO)"The dissimilatory mechanism relied on synergistic metabolism and functional complementation between NC1 and DC1, mediated by their respective functional genes."
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Resolve the full NDC-6 medium formulation, nitrogen concentrations used in the optimized assay, assay duration at the response-surface optimum, strain isolation histories, vessel geometry, and the detailed NC1/DC1 compatibility protocol from the version of record.
The indexed PubMed abstract identifies all six NDC-6 member strains, the NC1 and DC1 module design, the 1:1 inoculation ratio, sodium succinate as the optimized carbon source, and optimized pH, temperature, C/N ratio, and shaking speed. The accessible abstract does not report the full medium composition, source habitats of the individual strains, the optimized-assay duration, or vessel details.
Attaches to: taxonomy, growth_media, cultivation_setup
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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PubMed entry for the NDC-6 psychrotolerant nitrogen-removal SynCom
PubMed record for Dong et al. 2026. |
OTHER | PMID:41504900 |
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DOI for the NDC-6 psychrotolerant nitrogen-removal SynCom article
Version-of-record DOI for Dong et al. 2026. |
OTHER | doi:10.1007/s00449-025-03276-5 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| response-surface optimized NDC-6 conditions | C/N ratio 6, temperature 10.2 °C, pH 7.2, shaking speed 156 rpm | N/A |
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Growth Media
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