Nasal SynCom C Bioreactor

Community ID CommunityMech:000395

Ecological State ENGINEERED

Environment continuous nasal SynCom bioreactor — ontology label: laboratory environment
ENVO:01001405 · HabitatMech ↗

A nine-strain synthetic nasal community assembled from LaCa collection isolates recovered from Volunteer 1 and cultivated in a continuously fed glass bioreactor with synthetic nasal medium to maintain a stable S. aureus-free nasal microbiome before Day-20 S. aureus strain challenges.

Taxonomy

Taxon	Ontology ID	Abundance
Staphylococcus epidermidis Se C — ontology label: Staphylococcus epidermidis	NCBITaxon:1282 · TaxonMech · TraitMech	N/A
doi:10.64898/2026.03.16.712028 - SUPPORT (IN_VITRO) "Staphylococcus epidermidis Se C Vol. C BHI Overnight Aerobiosis Included"
Staphylococcus capitis Sc C — ontology label: Staphylococcus capitis	NCBITaxon:29388 · TaxonMech · TraitMech	N/A
doi:10.64898/2026.03.16.712028 - SUPPORT (IN_VITRO) "Staphylococcus capitis Sc C Vol. C BHI Overnight Aerobiosis Included"
Micrococcus luteus Ml C — ontology label: Micrococcus luteus	NCBITaxon:1270 · TaxonMech · TraitMech	N/A
doi:10.64898/2026.03.16.712028 - SUPPORT (IN_VITRO) "Micrococcus luteus Ml C Vol. C BHI Overnight Aerobiosis Included"
Corynebacterium tuberculostearicum Ct C — ontology label: Corynebacterium tuberculostearicum	NCBITaxon:38304 · TaxonMech · TraitMech	N/A
doi:10.64898/2026.03.16.712028 - SUPPORT (IN_VITRO) "Corynebacterium tuberculostearicum Ct C Vol. C BHI + 0.2% Tween 80 1 day Aerobiosis Included"
Cutibacterium avidum Cv C — ontology label: Cutibacterium avidum	NCBITaxon:33010 · TaxonMech · TraitMech	N/A
doi:10.64898/2026.03.16.712028 - SUPPORT (IN_VITRO) "Cutibacterium avidum Cv C Vol. C TSB 2 days Anaerobiosis Included"
Cutibacterium acnes Cc C — ontology label: Cutibacterium acnes	NCBITaxon:1747 · TaxonMech · TraitMech	N/A
doi:10.64898/2026.03.16.712028 - SUPPORT (IN_VITRO) "Cutibacterium acnes Cc C Vol. C TSB 4 days Anaerobiosis Included"
Cutibacterium granulosum Cg C — ontology label: Cutibacterium granulosum	NCBITaxon:33011 · TaxonMech · TraitMech	N/A
doi:10.64898/2026.03.16.712028 - SUPPORT (IN_VITRO) "Cutibacterium granulosum Cg C Vol. C TSB 4 days Anaerobiosis Included"
Anaerococcus octavius Ao C — ontology label: Anaerococcus octavius	NCBITaxon:54007 · TaxonMech · TraitMech	N/A
doi:10.64898/2026.03.16.712028 - SUPPORT (IN_VITRO) "Anaerococcus octavius Ao C Vol. C TSA + 5% sheep blood 7 days Anaerobiosis Included"
Peptoniphilus harei Ph C — ontology label: Peptoniphilus harei	NCBITaxon:54005 · TaxonMech · TraitMech	N/A
doi:10.64898/2026.03.16.712028 - SUPPORT (IN_VITRO) "Peptoniphilus harei Ph C Vol. C TSA + 5% sheep blood 7 days Anaerobiosis Included"

Ecological Interactions

ℹ️ No detailed ecological interaction data is available for this community.

This record may be a metabolic model or minimal entry containing only taxonomy and external resource links.

Associated Datasets

Dataset	Type	Repository	Accession
Continuous nasal bioreactor sequencing BioProject NCBI BioProject for sequencing datasets generated and analyzed across the continuous nasal bioreactor study, including the SynCom C S. aureus decolonization experiment.	AMPLICON_OTHER	NCBI_BIOPROJECT	PRJNA1414135

External Resources

Name	Repository	Resource ID
Primary biorxiv preprint - continuous nasal SynCom C bioreactor bioRxiv preprint reporting the SynCom C continuous bioreactor and S. aureus challenge experiment.	OTHER	doi:10.64898/2026.03.16.712028
doi:10.64898/2026.03.16.712028 - SUPPORT (IN_VITRO) "Development of a continuous bioreactor to maintain stable nasal microbiomes from swab specimens and synthetic communities"

Environmental Factors

Factor	Value	Unit
Day-20 Staphylococcus aureus challenge	Sa C, Sa A, or no S. aureus after 20 days of SynCom C stabilization	N/A
doi:10.64898/2026.03.16.712028 - SUPPORT (IN_VITRO) "We first maintained stable nasal microbiomes of the SynCom without S. aureus" doi:10.64898/2026.03.16.712028 - SUPPORT (IN_VITRO) "we added two different S. aureus strains ( i.e. , Sa C or Sa A) (Table S2) to the bioreactor on Day 20"

Growth Media

synthetic nasal medium 3

pH 6.5

Temperature 30 degrees Celsius

Atmosphere MICROAEROBIC

Preparation notes: Synthetic nasal medium 3 was supplied at the optimized 1 d-1 dilution rate, 30 degrees Celsius, and pH 6.5 in continuous mode. SynCom C was inoculated on Day 0 and stabilized to Day 20 before S. aureus challenge.

Evidence

doi:10.64898/2026.03.16.712028

"a continuous mode, a dilution rate of 1 d-1"

doi:10.64898/2026.03.16.712028

"a temperature of 30xlink:href=" a pH of 6.5, and a medium concentration with SNM 3"

doi:10.64898/2026.03.16.712028

"a pH of 6.5, and a medium concentration with SNM 3"

doi:10.64898/2026.03.16.712028

"We inoculated 1 mL of fresh nasal-swab specimens or 2.5 mL of the SynCom (OD600nm=1) into the bioreactor on Day 0."

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: continuous nasal SynCom bioreactor ↗ TaxonMech: Staphylococcus epidermidis Se C ↗ TraitMech: Staphylococcus epidermidis Se C ↗ TaxonMech: Staphylococcus capitis Sc C ↗ TraitMech: Staphylococcus capitis Sc C ↗ TaxonMech: Micrococcus luteus Ml C ↗ TraitMech: Micrococcus luteus Ml C ↗ TaxonMech: Corynebacterium tuberculostearicum Ct C ↗ TraitMech: Corynebacterium tuberculostearicum Ct C ↗ TaxonMech: Cutibacterium avidum Cv C ↗ TraitMech: Cutibacterium avidum Cv C ↗ TaxonMech: Cutibacterium acnes Cc C ↗ TraitMech: Cutibacterium acnes Cc C ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗