A defined two-member Ogataea polymorpha synthetic yeast coculture pairing a met10Δ-mCherry auxotroph, FY34, with a str3Δ-EGFP auxotroph, FY31. The pair was selected from systematic auxotroph screening because it showed reproducible growth restoration in Delft minimal medium with glucose or methanol; single-strain supplementation assays supported an asymmetric sulfur-metabolite dependency in which met10Δ could use multiple sulfur-containing intermediates while str3Δ depended on homocysteine.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Ogataea polymorpha FY34 met10Δ-mCherry — ontology label: Ogataea polymorpha | NCBITaxon:460523 · TaxonMech · TraitMech |
CROSS_FEEDER
|
COMMON |
|
|||
| Ogataea polymorpha FY31 str3Δ-EGFP — ontology label: Ogataea polymorpha | NCBITaxon:460523 · TaxonMech · TraitMech |
CROSS_FEEDER
|
COMMON |
|
|||
Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
MET10-STR3 Sulfur Auxotrophy Cross-Feeding
MUTUALISMThe met10Delta-str3Delta O. polymorpha pair showed reproducible growth complementation in Delft minimal medium on glucose and methanol. The reported glucose maximum OD600 range 27.3-28.5 spans this pair and str3Delta-leu2Delta, with no significant advantage over FY00 (26.8); methanol growth remained below FY00. FY34 met10Delta-mCherry and FY31 str3Delta-EGFP were the labeled derivatives used for population tracking, not identities established for every screening assay. Cooperative rescue is observed; specific sulfur exchange remains inferred.
Participating Taxa: Ogataea polymorpha FY34 met10Δ-mCherry, Ogataea polymorpha FY31 str3Δ-EGFP
Evidence
-
PMID:42318495 - SUPPORT (IN_VITRO)"The met10Δ and str3Δ mutants harbor deletions in the sulfur assimilation pathway at MET10 and STR3, respectively."
-
PMID:42318495 - SUPPORT (IN_VITRO)"The strongly complementary pairs (met10Δ-str3Δ and str3Δ-leu2Δ) reached maximum OD600 values of 27.3–28.5"
Asymmetric Homocysteine Dependency
CROSS_FEEDINGHYPOTHESIZED - The model proposes homocysteine supply from met10Delta to str3Delta and multiple organic sulfur intermediates in the reverse direction. Single-strain supplementation at 20 mg/L restored met10Delta growth with L-cysteine, cystathionine or homocysteine, but not sodium sulfide; str3Delta recovered substantially only with homocysteine among the tested compounds. These are demonstrated nutrient-rescue profiles, not measured interstrain transfer, secretion, exchange rates or an exhaustive nutrient requirement. The paper calls for isotope labeling or targeted metabolomics to identify coculture exchange.
Source Taxon: Ogataea polymorpha FY34 met10Δ-mCherry
Target Taxon: Ogataea polymorpha FY31 str3Δ-EGFP
Evidence
-
PMID:42318495 - PARTIAL (IN_VITRO)"met10Δ can utilize multiple sulfur-containing intermediates, whereas str3Δ relies exclusively on homocysteine."
-
PMID:42318495 - SUPPORT (IN_VITRO)"met10Δ recovered growth in the presence of L-Cys, Cysta, or Hcy, but not with Na2S. str3Δ, by contrast, showed substantial growth recovery only with Hcy."
Initial Ratio-Dependent Met10 Enrichment
Controlled initial met10Delta:str3Delta ratios at total OD600 0.4 changed growth and composition on glucose. Ratios 1:10, 1:5 and 1:1 achieved higher final biomass, with met10Delta fractions increasing; 5:1 and 10:1 maintained met10Delta dominance but grew weakly. Endpoint composition was generally met10Delta-biased at 168 h, not evidence that every culture increased its met10Delta fraction, grew robustly or converged to one identical ratio. Longer-term continuous and product-producing stability was not established.
Participating Taxa: Ogataea polymorpha FY34 met10Δ-mCherry, Ogataea polymorpha FY31 str3Δ-EGFP
Evidence
-
PMID:42318495 - SUPPORT (IN_VITRO)"Communities with initially higher frequencies of str3Δ (1:10, 1:5, and 1:1 groups) achieved higher final OD600 values."
-
PMID:42318495 - SUPPORT (IN_VITRO)"In contrast, groups initially dominated by met10Δ (5:1 and 10:1) showed weak growth throughout."
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which metabolites are actually exchanged, and how do they mediate the tested initial-ratio effect on growth?
The full paper explicitly infers directional exchange from single-strain supplement profiles and proposes tracers/metabolomics. Figure schematics are not transfer measurements. Growth restoration and ratio effects remain supported; distinct input/outcome and proposed exchange-to-rescue topology remain a research task. Prior dangling phenotype arrows remain absent. One-record Edison dry run is not an inspected provider report or paid authorization.
Attaches to: ecological_interactions#MET10-STR3 Sulfur Auxotrophy Cross-Feeding, ecological_interactions#Asymmetric Homocysteine Dependency, ecological_interactions#Initial Ratio-Dependent Met10 Enrichment
1 evidence item(s)
The isotopic labeling or targeted metabolomics would help to directly identify the specific metabolites exchanged in co-culture
Explicitly identifies the unresolved exchange measurement.
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Primary publication - Ogataea sulfur cross-feeding coculture
Synthetic and Systems Biotechnology article reporting the Ogataea polymorpha met10Δ-str3Δ auxotrophic cross-feeding consortium. |
OTHER | PMID:42318495 |
|
||
|
DOI for the Ogataea sulfur cross-feeding article
Version-of-record DOI for the 2026 Synthetic and Systems Biotechnology Ogataea cross-feeding article. |
OTHER | doi:10.1016/j.synbio.2026.05.008 |
|
||
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| initial met10Δ:str3Δ ratio | N/A | |
|
||
Growth Media
Cross-Mech Suite Links
Explore related entities across the CultureBotAI X-Mech knowledge bases: