Poultry-Wastewater Three-Strain Electroactive Consortium

Community ID CommunityMech:000330

Ecological State ENGINEERED

Environment poultry-wastewater microbial fuel-cell laboratory system
ENVO:01001405

Modeled Environment poultry slaughterhouse waste water
ENVO:00002001

A defined equal-volume consortium of Lysinibacillus sphaericus A1, Bacillus cereus A2, and Lactococcus lactis A4 tested in dual-chamber microbial fuel cells containing amended, sterilized poultry slaughterhouse wastewater. The consortium produced more power and removed more chemical oxygen demand than the constituent strains tested separately during the 30-day experiment.

Taxonomy

Taxon	Ontology ID	Functional Roles	Abundance
Lysinibacillus sphaericus A1	NCBITaxon:1421	ELECTROGEN	N/A
PMID:41594859 - SUPPORT (IN_VITRO) "Three electroactive bacterial strains were used in this study: Lysinibacillus sphaericus A1, Bacillus cereus A2, and Lactococcus lactis A4."
Bacillus cereus A2	NCBITaxon:1396	ELECTROGEN	N/A
PMID:41594859 - SUPPORT (IN_VITRO) "Three electroactive bacterial strains were used in this study: Lysinibacillus sphaericus A1, Bacillus cereus A2, and Lactococcus lactis A4."
Lactococcus lactis A4	NCBITaxon:1358	ELECTROGEN	N/A
PMID:41594859 - SUPPORT (IN_VITRO) "Three electroactive bacterial strains were used in this study: Lysinibacillus sphaericus A1, Bacillus cereus A2, and Lactococcus lactis A4."

Ecological Interactions

Taxon

Niche partitioning

Complementary electroactive consortium performance

NICHE_PARTITIONING

Evidence

PMID:41594859 - SUPPORT (IN_VITRO)

"selecting complementary strains with diverse metabolic functions can substantially improve system performance"
PMID:41594859 - SUPPORT (IN_VITRO)

"Compared with single cultures, the consortium produced approximately 15% higher power density than L. sphericus A1, about 29% higher than B. cereus A2, and more than threefold higher than L. lactis A4."

External Resources

Name	Repository	Resource ID
Primary publication - poultry-wastewater electroactive consortium Primary laboratory microbial fuel-cell comparison of the exact three-strain consortium and its individual members.	OTHER	PMID:41594859
PMID:41594859 - SUPPORT (IN_VITRO) "Lysinibacillus sphericus A1, Bacillus cereus A2 and Lactococcus lactis A4, compared with a developed consortium"

Environmental Factors

Factor	Value	Unit
Operation duration	30	days
PMID:41594859 - SUPPORT (IN_VITRO) "All inocula were tested in dual-chamber MFCs for 30 days"
Maximum consortium power density	170	mW/m2
PMID:41594859 - SUPPORT (IN_VITRO) "The consortium showed the highest power of 170 mW/m2"
Chemical oxygen demand removal	84.4	percent
PMID:41594859 - SUPPORT (IN_VITRO) "The highest COD removal (84.4%) was also recorded"

Growth Media

Inoculum preparation medium

Temperature 37 C

Composition

Component	Concentration	Unit	CHEBI
Luria-Bertani broth	10	percent	N/A

Preparation notes: Each strain was first revived on nutrient meat broth for 24 h at 37 C, then cultivated overnight in 10% Luria-Bertani broth and standardized to OD600 0.8 +/- 0.05.

Evidence

PMID:41594859

"For inoculum preparation, the strains were cultivated in 10% Luria–Bertani broth"

PMID:41594859

"on a rotary shaker at 150 rpm and 37 °C overnight"

Amended poultry slaughterhouse wastewater anolyte

pH 7.0 +/- 0.1

Composition

Component	Concentration	Unit	CHEBI
poultry slaughterhouse wastewater	N/A	N/A	N/A
glucose	3	g/L	N/A
tryptone	10	g/L	N/A
yeast extract	5	g/L	N/A

Preparation notes: The amended wastewater was autoclaved at 121 C for 20 min, making it a sterilized model substrate rather than untreated slaughterhouse wastewater.

Evidence

PMID:41594859

"the wastewater was supplemented with glucose (3 g/L"

PMID:41594859

"tryptone (10 g/L"

PMID:41594859

"yeast extract (5 g/L"

PMID:41594859

"The pH of the amended wastewater was adjusted to 7.0 ± 0.1."