A defined two-member synthetic microbial community composed of Priestia flexa and Pseudomonas putida that mitigates arsenic toxicity in rice (Oryza sativa var. Sarju-52). In arsenite- and arsenate-stressed paddy-soil assays, SynCom inoculation restored plant photosynthetic and metabolic balance, limited oxidative-stress responses, reduced arsenic accumulation in roots, shoots and grains, and coordinately down-regulated rice arsenic-transporter genes including Lsi1, Lsi2, Lsi3, OsNIP1;1, and OsNIP3;3.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
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| Priestia flexa | NCBITaxon:86664 · TaxonMech · TraitMech | DOMINANT | |
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| Pseudomonas putida | NCBITaxon:303 · TaxonMech · TraitMech | DOMINANT | |
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| Oryza sativa var. Sarju-52 — ontology label: Oryza sativa | NCBITaxon:4530 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
SynCom restriction of rice arsenic accumulation
The Priestia flexa and Pseudomonas putida SynCom reduced arsenic accumulation in rice roots, shoots and grains. PARTIAL: the abstract attributes this effect to coordinated transporter-gene downregulation; accessible evidence does not establish transporter necessity, individual-strain contribution or reciprocal microbial benefit.
Participating Taxa: Priestia flexa, Pseudomonas putida, Oryza sativa var. Sarju-52
Biological Processes:
- response to toxic substance (GO:0009636 · PathwayMech)
Evidence
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PMID:42691895 - SUPPORT (IN_VIVO)"SynCom substantially reduced arsenic accumulation in roots, shoots and grains by coordinate downregulation of arsenic transporter genes"
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PMID:42691895 - SUPPORT (IN_VIVO)"Lsi1, Lsi2, Lsi3, OsNIP1;1, and OsNIP3;3"
SynCom restoration of rice stress physiology
SynCom inoculation improved photosynthesis and soluble sugars and reduced proline accumulation and antioxidant-enzyme overactivation under arsenic stress. Preserve these positive rice responses without asserting reciprocal mutualism or an untested causal direction between these composite outcome nodes.
Participating Taxa: Priestia flexa, Pseudomonas putida, Oryza sativa var. Sarju-52
Biological Processes:
- response to stress (GO:0006950 · PathwayMech)
Evidence
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PMID:42691895 - SUPPORT (IN_VIVO)"Inoculation with SynCom restored physiological function and metabolic balance, as evidenced by improved photosynthesis, increased soluble sugars, and reduced proline accumulation."
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which bacterial activities and host transporters mediate the SynCom benefit?
The accessible abstract supports two-member inoculation, arsenic restriction and host physiological improvement. Full text was unavailable. It does not establish reciprocal benefit, strain necessity or a direction between the two existing outcome nodes. Transporter downregulation remains the authors explanation, with causal mediation qualified; no new structure is needed for this repair.
Attaches to: ecological_interactions#SynCom restriction of rice arsenic accumulation, ecological_interactions#SynCom restoration of rice stress physiology
2 evidence item(s)
SynCom substantially reduced arsenic accumulation in roots, shoots and grains by coordinate downregulation of arsenic transporter genes
Supports the reported whole-SynCom endpoint and proposed transporter explanation, not mediator-specific intervention proof.
Inoculation with SynCom restored physiological function and metabolic balance, as evidenced by improved photosynthesis, increased soluble sugars, and reduced proline accumulation.
Supports improved host photosynthetic and metabolic performance under arsenic stress after SynCom inoculation.
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Primary publication - rice arsenic-stress Priestia-Pseudomonas SynCom
Microbiological Research 2026 primary article describing the Priestia-Pseudomonas SynCom that restricts arsenic accumulation in rice. |
OTHER | PMID:42691895 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Arsenite and arsenate stress | As(III), 18 mg/kg; As(V), 50 mg/kg | N/A |
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