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Pseudomonas-Acidovorax Aniline-Degradation SynCom

Community ID CommunityMech:000461
Ecological State ENGINEERED
Environment aniline-fed laboratory shake-flask coculture — ontology label: laboratory environment
ENVO:01001405 · HabitatMech ↗
Modeled Environment aniline-contaminated industrial soil — ontology label: contaminated soil
ENVO:00002116 · HabitatMech ↗

A two-member Pseudomonas sp./Acidovorax sp. synthetic microbial consortium, RF-PH, assembled from the aniline-degrading strains RF and PH isolated from heavily aniline-contaminated soil in a Dalian chemical industrial park. Pan et al. 2026 identified Pseudomonas and Acidovorax isolates by 16S rRNA sequencing, optimized their inoculation ratio in mineral-salts medium containing aniline, and designated a 3:1 RF:PH mixture as RF-PH after the consortium outperformed either monoculture in aniline shake-flask assays.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Pseudomonas sp. RF — ontology label: Pseudomonas NCBITaxon:286 · TaxonMech · TraitMech
PRIMARY_DEGRADER
N/A
  • PMID:41900437 - SUPPORT (IN_VITRO)
    "Identification via 16S rRNA gene sequencing and BLAST analysis confirmed the strains belonged to the genera Pseudomonas and Acidovorax, respectively."
  • PMID:41900437 - SUPPORT (IN_VITRO)
    "Pseudomonas sp. RF completely degraded 500 mg·L−1 aniline within 18 h and achieved complete degradation of 1000 mg·L−1 aniline within 24 h"
  • PMID:41900437 - SUPPORT (OTHER)
    "The 16S rRNA gene sequences of strains PH and RF have been deposited in GenBank under accession numbers PX736765 and PX736766"
Acidovorax sp. PH — ontology label: Acidovorax NCBITaxon:12916 · TaxonMech · TraitMech
PRIMARY_DEGRADER
N/A
  • PMID:41900437 - SUPPORT (IN_VITRO)
    "Identification via 16S rRNA gene sequencing and BLAST analysis confirmed the strains belonged to the genera Pseudomonas and Acidovorax, respectively."
  • PMID:41900437 - SUPPORT (IN_VITRO)
    "Acidovorax sp. PH achieved 100% and 97.31% degradation rates for 500 mg·L−1 and 1000 mg·L−1 aniline, respectively, within 24 h"
  • PMID:41900437 - SUPPORT (OTHER)
    "The 16S rRNA gene sequences of strains PH and RF have been deposited in GenBank under accession numbers PX736765 and PX736766"

Ecological Interactions

The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.

Ecological interaction network for Pseudomonas-Acidovorax Aniline-Degradation SynCom Bipartite graph where circles represent taxa and non-circular symbols represent interactions. Rare or untyped interactions share the Other symbol; exact curated types remain in the interaction details.
Taxon
Other

RF-PH enhanced aniline degradation

The optimized 3:1 RF-PH consortium degraded 500 mg/L aniline more rapidly within 12 h than either single-strain culture, and also improved 12 h removal at 1000 mg/L aniline. These analytical removal outcomes do not by themselves establish complete mineralization, a catechol pathway or a directional metabolite exchange between strains.

Participating Taxa: Pseudomonas sp. RF, Acidovorax sp. PH

Evidence

  • PMID:41900437 - SUPPORT (IN_VITRO)
    "For 500 mg·L−1 aniline, the degradation rate reached 91.28% within 12 h, representing an increase of 11.33% and 17.02% compared to the degradation rates achieved by strain RF or strain PH alone, respectively."
  • PMID:41900437 - SUPPORT (IN_VITRO)
    "For 1000 mg·L−1 aniline, the degradation rate reached 73.06% within 12 h, representing an increase of 18.25% and 28.04% compared to the degradation by strain RF or strain PH alone, respectively."

Knowledge gaps & discussions (2)

KNOWLEDGE_GAP OPEN Which molecular mechanisms, if any, connect Pseudomonas sp. RF and Acidovorax sp. PH to enhanced RF-PH aniline degradation?

Pan et al. 2026 reported that RF-PH outperformed either monoculture and proposed cooperative and complementary characteristics of the two strains, but did not experimentally identify a transferred intermediate or other directional pairwise mechanism.

Attaches to: ecological_interactions#RF-PH enhanced aniline degradation

KNOWLEDGE_GAP OPEN Does the analytical removal endpoint establish the asserted mineralization pathway?

The positive consortium-versus-monoculture comparisons remain supported. Main methods quantify residual aniline, while the conclusion asserts catechol-pathway mineralization without corresponding mechanistic results in the main text reviewed. Supplements were not inspected, so this is an evidence boundary rather than proof that no additional data exist. No transferred intermediate, competition or new arrow is inferred; the existing open-ended mechanism discussion is preserved.

Attaches to: ecological_interactions#RF-PH enhanced aniline degradation

2 evidence item(s)
IN_VITRO SUPPORT PMID:41900437

For 500 mg·L−1 aniline, the degradation rate reached 91.28% within 12 h, representing an increase of 11.33% and 17.02% compared to the degradation rates achieved by strain RF or strain PH alone, respectively.

Supports the community-level enhancement of aniline degradation by RF-PH relative to either monoculture.

IN_VITRO SUPPORT PMID:41900437

For 1000 mg·L−1 aniline, the degradation rate reached 73.06% within 12 h, representing an increase of 18.25% and 28.04% compared to the degradation by strain RF or strain PH alone, respectively.

Supports the RF-PH consortium's enhanced 1000 mg/L aniline degradation relative to either monoculture.

External Resources

Name Repository Resource ID
Primary publication - RF-PH aniline-degradation SynCom
Microorganisms 2026 open-access primary article, doi:10.3390/microorganisms14030678, describing the two-member RF-PH Pseudomonas/Acidovorax aniline-degradation SynCom. 		OTHER PMID:41900437
  • PMID:41900437 - SUPPORT (IN_VITRO)
    "Construction of a Synthetic Aniline-Degrading Consortium Consisting of Pseudomonas sp. RF and Acidovorax sp. PH Guided by Soil Niche Information from Contaminated Sites"

Environmental Factors

Factor Value Unit
RF-PH inoculation ratio RF:PH = 3:1 biomass ratio
  • PMID:41900437 - SUPPORT (IN_VITRO)
    "The maximum aniline degradation rate obtained in the experiments was 93.28%, from Experiment No. 2, where the inoculation ratio of strains RF to PH was 3:1."
aniline assay substrate aniline 500 mg/L
  • PMID:41900437 - SUPPORT (IN_VITRO)
    "In 100 mL of mineral salt medium containing 500 mg·L−1 aniline, bacterial resuspensions were added according to the ratios outlined, followed by shake-flask cultivation at 150 r·min−1 for 3 days."

Growth Media

RF-PH mineral-salts aniline shake-flask medium

pH 7.0

Composition

Component Concentration Unit CHEBI
aniline · MediaIngredientMech 500 mg/L N/A
MgSO4.7H2O · MediaIngredientMech 0.2 g/L N/A
CaCl2 · MediaIngredientMech 0.01 g/L N/A
FeSO4.7H2O · MediaIngredientMech 0.001 g/L N/A
KH2PO4 · MediaIngredientMech 1.5 g/L N/A
Na2HPO4 · MediaIngredientMech 1.00 g/L N/A
MnSO4 · MediaIngredientMech 0.02 g/L N/A
NH4Cl · MediaIngredientMech 1 g/L N/A

Preparation notes: RF and PH were grown in LB to late logarithmic phase, washed, resuspended in sterile water, adjusted to OD600 2.0, and added to 100 mL mineral salt medium containing aniline.

Evidence

PMID:41900437

"In 100 mL of mineral salt medium containing 500 mg·L−1 aniline, bacterial resuspensions were added according to the ratios outlined, followed by shake-flask cultivation at 150 r·min−1 for 3 days."

PMID:41900437

"MgSO4·7H2O (0.2 g·L−1), CaCl2 (0.01 g·L−1), FeSO4·7H2O (0.001 g·L−1), KH2PO4 (1.5 g·L−1), Na2HPO4 (1.00 g·L−1), MnSO4 (0.02 g·L−1), and NH4Cl (1 g·L−1)"

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: aniline-fed laboratory shake-flask coculture ↗ TaxonMech: Pseudomonas sp. RF ↗ TraitMech: Pseudomonas sp. RF ↗ TaxonMech: Acidovorax sp. PH ↗ TraitMech: Acidovorax sp. PH ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗