A full-factorial synthetic community panel built from eight Pseudomonas aeruginosa mutants: pAOV, PA14, NfxB, ParR-CAZ, mexZ, OrfN, NfxB-CAZ, and MDR6-CAZ. Diaz-Colunga et al. assembled all 256 strain combinations in LB medium in 96-well plates, measured post-growth absorbance spectra after 40 h at 37 C, and identified the three-member 00001101 consortium containing strains 1, 3, and 4 as the highest-Abs600 combination.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
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| Pseudomonas aeruginosa pAOV — ontology label: Pseudomonas aeruginosa | NCBITaxon:287 · TaxonMech · TraitMech | N/A | |
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| Pseudomonas aeruginosa PA14 — ontology label: Pseudomonas aeruginosa | NCBITaxon:287 · TaxonMech · TraitMech | N/A | |
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| Pseudomonas aeruginosa NfxB — ontology label: Pseudomonas aeruginosa | NCBITaxon:287 · TaxonMech · TraitMech | N/A | |
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| Pseudomonas aeruginosa ParR-CAZ — ontology label: Pseudomonas aeruginosa | NCBITaxon:287 · TaxonMech · TraitMech | N/A | |
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| Pseudomonas aeruginosa mexZ — ontology label: Pseudomonas aeruginosa | NCBITaxon:287 · TaxonMech · TraitMech | N/A | |
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| Pseudomonas aeruginosa OrfN — ontology label: Pseudomonas aeruginosa | NCBITaxon:287 · TaxonMech · TraitMech | N/A | |
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| Pseudomonas aeruginosa NfxB-CAZ — ontology label: Pseudomonas aeruginosa | NCBITaxon:287 · TaxonMech · TraitMech | N/A | |
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| Pseudomonas aeruginosa MDR6-CAZ — ontology label: Pseudomonas aeruginosa | NCBITaxon:287 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Highest-Abs600 00001101 trio
The highest Abs600 value in the demonstrator experiment came from consortium 00001101, the three-mutant Pseudomonas aeruginosa combination containing pAOV, NfxB, and ParR-CAZ; its positive third-order functional interaction is statistical under the source's additive Abs600 baseline, not a resolved molecular competition mechanism. The ranking is illustrative, with similarly high values in other consortia; absorbance is not a linear biomass measure across its full range.
Participating Taxa: Pseudomonas aeruginosa pAOV, Pseudomonas aeruginosa NfxB, Pseudomonas aeruginosa ParR-CAZ
Evidence
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PMID:42153623 - SUPPORT (IN_VITRO)"the consortium with the highest function contains only three strains (consortium 00001101, containing strains 1, 3, and 4)"
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PMID:42153623 - SUPPORT (IN_VITRO)"the three strains engage in a positive third- order functional interaction (see inset in Figure 5E) which rescues the function of the trio"
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PMID:42153623 - SUPPORT (IN_VITRO)"Under this definition, the term interaction should be interpreted in a statistical, rather than biological sense"
Knowledge gaps & discussions (2)
KNOWLEDGE_GAP OPEN What evidence, if any, resolves a biological mechanism behind the statistical phenotype?
The eLife study quantified functional effects and interaction coefficients from Abs600 phenotypes, but did not identify the molecular mechanism behind the positive third-order interaction in the high-Abs600 trio.
Attaches to: ecological_interactions#Highest-Abs600 00001101 trio
1 evidence item(s)
This observation highlights the potential of full factorial design to interrogate and generate hypotheses with respect to the mechanisms that govern the emergence of community- level functions
The source frames mechanism generation as downstream of the functional factorial measurement rather than resolved by it.
KNOWLEDGE_GAP OPEN How should an assay panel be distinguished from an individual-community interaction?
Experimental assembly is a method, not a biological interaction, and was removed from the graph. The existing statistical phenotype remains, without new biological mechanisms or experimental designs. Five canonical panel members lose the artificial connectivity supplied by the method node; their membership representation remains #1729. Relevant cached primary results and discussion were read, but fresh full-text retrieval failed. This review does not independently certify the entire cached paper, raw data or figure pixels.
Attaches to: ecological_interactions#Highest-Abs600 00001101 trio
1 evidence item(s)
Under this definition, the term interaction should be interpreted in a statistical, rather than biological sense
Clarifies that the high-Abs600 trio's interaction coefficient is a statistical deviation from an additive function baseline, not a resolved biological competition mechanism.
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Full-factorial construction of synthetic microbial communities
Primary eLife article describing the P. aeruginosa eight-mutant SynCom panel. |
OTHER | PMID:42153623 |
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Growth Media
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