A defined two-member consortium of Pseudomonas aeruginosa PA12 and Bacillus subtilis G1 engineered to biodegrade waxy crude oil. Bacillus improves access to the oil-water interface and supplies metabolic precursors, while Pseudomonas performs deeper oxidation of long-chain alkanes and aromatics.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Pseudomonas aeruginosa PA12 — ontology label: Pseudomonas aeruginosa | NCBITaxon:287 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
CROSS_FEEDER
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ABUNDANT |
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| Bacillus subtilis G1 — ontology label: Bacillus subtilis | NCBITaxon:1423 · TaxonMech · TraitMech |
CROSS_FEEDER
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ABUNDANT |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Interfacial Remodeling and Precursor Cross-feeding
CROSS_FEEDINGPARTIAL: the authors propose that Bacillus-mediated interface remodeling and precursor supply facilitate the partner contribution to waxy-oil conversion. Preserve this reported directed interpretation; the accessible abstract does not establish a measured transfer flux or isolate each mediator experimentally.
Source Taxon: Bacillus subtilis G1
Target Taxon: Pseudomonas aeruginosa PA12
Metabolites: alkane (CHEBI:18310 · MediaIngredientMech · CMMMech)
Biological Processes:
- alkane catabolic process (GO:0043448 · PathwayMech)
PARTIAL: interface remodeling and precursor transfer are proposed contributors to the reported cooperative degradation model. The abstract supports this interpretation but does not establish their necessity or relative contributions.
Evidence
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PMID:41905048 - SUPPORT (IN_VITRO)"enhancing substrate accessibility and supplying precursors to PA12"
Multilayer Cooperative Hydrocarbon Degradation
PARTIAL: multi-omics and molecular docking support the authors multilayer cooperation model. These analyses do not by themselves prove functional mediation by each proposed process or reciprocal fitness benefits; the measured consortium-versus-monoculture degradation advantage remains a separate positive result.
Participating Taxa: Pseudomonas aeruginosa PA12, Bacillus subtilis G1
Biological Processes:
- quorum sensing (GO:0009372 · PathwayMech)
PARTIAL: the proposed cooperative processes are offered as an explanation for the observed wax-removal advantage. The abstract does not resolve which processes are necessary or sufficient for that endpoint.
Evidence
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PMID:41905048 - SUPPORT (IN_VITRO)"a multilayer cooperative mechanism integrating interfacial remodeling, metabolic cross-feeding, signal exchange, and redox-energy homeostasis"
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PMID:41905048 - SUPPORT (IN_VITRO)"via aromatic intermediates, fatty acids, and quorum-sensing signals"
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PMID:41905048 - SUPPORT (IN_VITRO)"monocultures showed lower degradation efficiencies, reaching 52.5% for PA12 and 42.0% for G1"
High-Efficiency Waxy Oil Removal
The source reports 85.5% wax removal within 12 days, compared with lower monoculture degradation efficiencies of 52.5% and 42.0%. Preserve this positive treatment comparison without equating pollutant removal with reciprocal growth benefits or complete mineralization.
Participating Taxa: Pseudomonas aeruginosa PA12, Bacillus subtilis G1
Metabolites: alkane (CHEBI:18310 · MediaIngredientMech · CMMMech)
Biological Processes:
- alkane catabolic process (GO:0043448 · PathwayMech)
Evidence
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PMID:41905048 - SUPPORT (IN_VITRO)"achieving 85.5% wax removal within 12 d"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which reported cooperative processes actually mediate the removal advantage?
All three nodes and both existing directions remain. Positive removal measurements are distinct from multi-omics and docking interpretations. Only the fresh abstract was accessible; absence of full methods is not evidence that perturbations were absent. No new biological mechanisms, strain combinations, optimization parameters or experimental designs were added.
Attaches to: ecological_interactions#Interfacial Remodeling and Precursor Cross-feeding, ecological_interactions#Multilayer Cooperative Hydrocarbon Degradation, ecological_interactions#High-Efficiency Waxy Oil Removal
2 evidence item(s)
enhancing substrate accessibility and supplying precursors to PA12
Supports the authors proposed substrate-access and precursor-transfer contribution, not independently verified transfer flux.
Molecular docking validated the aforementioned mechanism of action
This is the authors wording; docking is computational support, not independent biological validation of the whole causal mechanism.
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Primary publication - PA12-G1 waxy oil consortium
Primary multi-omics study of the exact two-strain consortium. |
OTHER | PMID:41905048 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Waxy crude oil loading | 2 | % w/v |
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| Wax removal | 85.5 | percent within 12 days |
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Growth Media
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