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Pseudomonas-Bacillus Waxy Oil Biodegradation Consortium

Community ID CommunityMech:000324
Ecological State ENGINEERED
Environment waxy crude oil laboratory consortium
ENVO:01001405

A defined two-member consortium of Pseudomonas aeruginosa PA12 and Bacillus subtilis G1 engineered to biodegrade waxy crude oil. Bacillus improves access to the oil-water interface and supplies metabolic precursors, while Pseudomonas performs deeper oxidation of long-chain alkanes and aromatics.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Pseudomonas aeruginosa PA12 NCBITaxon:287
PRIMARY_DEGRADER CROSS_FEEDER
ABUNDANT
  • PMID:41905048 - SUPPORT (IN_VITRO)
    "Pseudomonas aeruginosa PA12-Bacillus subtilis G1 consortium"
  • PMID:41905048 - SUPPORT (IN_VITRO)
    "PA12 assimilates these intermediates, activating its alkB-fad-ben/cat metabolic axis"
Bacillus subtilis G1 NCBITaxon:1423
CROSS_FEEDER
ABUNDANT
  • PMID:41905048 - SUPPORT (IN_VITRO)
    "Pseudomonas aeruginosa PA12-Bacillus subtilis G1 consortium"
  • PMID:41905048 - SUPPORT (IN_VITRO)
    "G1 remodels the oil-water interface via lipopeptides and small-molecule metabolites"

Ecological Interactions

Ecological interaction network for Pseudomonas-Bacillus Waxy Oil Biodegradation Consortium Bipartite graph where circle nodes represent taxa and each ecological interaction is drawn as a distinct non-circular symbol, with colour repeating the same distinction (cross-feeding, mutualism).
Taxon
Cross-feeding
Mutualism

Interfacial Remodeling and Precursor Cross-feeding

CROSS_FEEDING

Source Taxon: Bacillus subtilis G1

Target Taxon: Pseudomonas aeruginosa PA12

Evidence

  • PMID:41905048 - SUPPORT (IN_VITRO)
    "enhancing substrate accessibility and supplying precursors to PA12"

Multilayer Cooperative Hydrocarbon Degradation

MUTUALISM

Evidence

  • PMID:41905048 - SUPPORT (IN_VITRO)
    "a multilayer cooperative mechanism integrating interfacial remodeling, metabolic cross-feeding, signal exchange, and redox-energy homeostasis"
  • PMID:41905048 - SUPPORT (IN_VITRO)
    "monocultures showed lower degradation efficiencies, reaching 52.5% for PA12 and 42.0% for G1"

External Resources

Name Repository Resource ID
Primary publication - PA12-G1 waxy oil consortium
Primary multi-omics study of the exact two-strain consortium. 		OTHER PMID:41905048
  • PMID:41905048 - SUPPORT (IN_VITRO)
    "a synergistic Pseudomonas aeruginosa PA12-Bacillus subtilis G1 consortium"

Environmental Factors

Factor Value Unit
Waxy crude oil loading 2 % w/v
  • PMID:41905048 - SUPPORT (IN_VITRO)
    "biodegrade waxy crude oil (2% w/v)"
Wax removal 85.5 percent within 12 days
  • PMID:41905048 - SUPPORT (IN_VITRO)
    "achieving 85.5% wax removal within 12 d"

Growth Media

Waxy crude oil biodegradation culture

Composition

Component Concentration Unit CHEBI
waxy crude oil 2 % w/v N/A

Preparation notes: The abstract does not report the basal medium composition, pH, temperature, or aeration conditions.

Evidence

PMID:41905048

"waxy crude oil (2% w/v), achieving 85.5% wax removal within 12 d"