A defined three-member synthetic bacterial consortium composed of the aerobic denitrifier Pseudomonas stutzeri, the facultative anaerobic denitrifier Klebsiella sp., and the heterotrophic nitrifying-aerobic denitrifying bacterium Alcaligenes sp. for Fe2+-regulated deep nitrogen removal. The 2026 Environmental Research abstract reports that the Fe2+ treatment increased nitrate-N removal efficiency by 20-30% relative to other iron species and that metagenomic analysis associated Fe2+ with a shift in carbon and nitrogen metabolic networks, including higher dissimilatory nitrate reduction to ammonium pathway abundance and lower denitrification pathway abundance.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Pseudomonas stutzeri — ontology label: Stutzerimonas stutzeri | NCBITaxon:316 · TaxonMech · TraitMech | N/A | |
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| Klebsiella sp. — ontology label: Klebsiella | NCBITaxon:570 · TaxonMech · TraitMech | N/A | |
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| Alcaligenes sp. — ontology label: Alcaligenes | NCBITaxon:507 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Composite nitrogen-removal metabolic network
The three-member consortium showed higher nitrate-N removal under the reported iron-species treatment comparison and associated changes in carbon/nitrogen pathway abundance. PARTIAL: complementary named capabilities do not establish niche partitioning, member-specific contributions or measured pathway flux. The abstract labels the analysis metagenomic but calls the reported counts transcript abundance, leaving the measurement type unresolved.
Participating Taxa: Pseudomonas stutzeri, Klebsiella sp., Alcaligenes sp.
Metabolites: nitrate (CHEBI:17632 · MediaIngredientMech · CMMMech), ammonium (CHEBI:28938 · MediaIngredientMech · CMMMech)
Biological Processes:
- denitrification (GO:0019333 · PathwayMech)
Evidence
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PMID:42520901 - SUPPORT (IN_VITRO)"comprising the aerobic denitrifier Pseudomonas stutzeri, the facultative anaerobic denitrifier Klebsiella sp., and the heterotrophic nitrifying-aerobic denitrifying bacterium Alcaligenes sp."
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PMID:42520901 - SUPPORT (COMPUTATIONAL)"Metagenomic analysis revealed that Fe2+ modulated the carbon and nitrogen metabolic networks: in the carbon metabolic network, enrichment of the por gene in the glycolytic pathway generated substantial reducing power in the form of reduced ferredoxin; concomitantly, the transcript abundance of the dissimilatory nitrate reduction to ammonium pathway increased from 775 to 802, whereas that of the denitrification pathway decreased from 1259 to 1222."
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PMID:42520901 - SUPPORT (IN_VITRO)"compared with the other iron species, the Fe2+ group achieved a 20-30% increase in nitrate-N removal efficiency"
Knowledge gaps & discussions (2)
KNOWLEDGE_GAP OPEN Which Pseudomonas stutzeri, Klebsiella sp., and Alcaligenes sp. strains, nitrogen source, iron doses, PAM dose, medium recipe, inoculation ratio, and incubation conditions were used to assemble and assay the Fe2+-regulated nitrogen-removal consortium?
PubMed exposes the abstract but PubMed Central does not link open full text for PMID:42520901. The abstract identifies the three member taxa and Fe2+/PAM perturbation design but not the strain designations or cultivation details needed for a fully reproducible setup.
Attaches to: engineering_design#assembly_strategy, growth_media#Fe2+-regulated nitrogen-removal assay
KNOWLEDGE_GAP OPEN What measurement supports the reported pathway shift and net nitrogen fate?
The nitrate-N treatment response remains a measured positive endpoint. The accessible abstract mixes metagenomic sequencing with transcript-abundance terminology; it does not resolve that inconsistency, taxon-specific partitioning or flux. Nitrate disappearance alone does not establish whole-system nitrogen loss. Full text and complete nitrogen balances were not available in this review. No new arrows or experimental protocols were added.
Attaches to: ecological_interactions#Composite nitrogen-removal metabolic network
2 evidence item(s)
Metagenomic analysis revealed that Fe2+ modulated the carbon and nitrogen metabolic networks: in the carbon metabolic network, enrichment of the por gene in the glycolytic pathway generated substantial reducing power in the form of reduced ferredoxin; concomitantly, the transcript abundance of the dissimilatory nitrate reduction to ammonium pathway increased from 775 to 802, whereas that of the denitrification pathway decreased from 1259 to 1222.
Supports the source-reported abundance shift with an unresolved metagenomic/transcript terminology inconsistency, not verified transcription, reducing-power production or nitrogen flux.
compared with the other iron species, the Fe2+ group achieved a 20-30% increase in nitrate-N removal efficiency
Supports Fe2+ as the iron species that increased nitrate-N removal efficiency.
External Resources
| Name | Repository | Resource ID |
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Primary publication - Fe2+-regulated nitrogen-removal SynCom
Environmental Research 2026 primary article, doi:10.1016/j.envres.2026.125349, describing a Pseudomonas-Klebsiella-Alcaligenes synthetic consortium and Fe2+ regulation of nitrate removal and carbon/nitrogen metabolic networks. |
OTHER | PMID:42520901 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Ferrous iron regulation | Fe2+ | iron-species treatment |
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| Polyacrylamide mass-transfer perturbation | polyacrylamide | PAM amendment |
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Growth Media
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