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Pseudomonas-Rhodococcus Chloronitrobenzene Coculture

Community ID CommunityMech:000181
Ecological State ENGINEERED
Environment chloronitrobenzene-fed laboratory coculture
ENVO:01001405

A defined two-member aerobic laboratory coculture for chloronitrobenzene bioremediation. Pseudomonas putida HS12 was isolated using nitrobenzene enrichment and performs the first partial reductive and acetylation steps on 3- and 4-chloronitrobenzene. Rhodococcus sp. strain HS51 degrades the chlorohydroxyacetanilide intermediates, allowing the coculture to mineralize 3- and 4-chloronitrobenzenes when an additional carbon source is supplied.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Pseudomonas putida HS12 NCBITaxon:303
PRIMARY_DEGRADER
N/A
  • PMID:10049867 - SUPPORT (IN_VITRO)
    "Pseudomonas putida HS12 was first isolated by analogue enrichment culture"
  • PMID:10049867 - SUPPORT (IN_VITRO)
    "this strain was observed to possess a partial reductive pathway for the degradation of NB"
Rhodococcus sp. strain HS51 NCBITaxon:1827
CROSS_FEEDER PRIMARY_DEGRADER
N/A
  • PMID:10049867 - SUPPORT (IN_VITRO)
    "Rhodococcus sp. strain HS51, which degrades 4- and 5-chloro-2-hydroxyacetanilides"
  • PMID:10049867 - SUPPORT (IN_VITRO)
    "was isolated and combined with P. putida HS12 to give a coculture"

Ecological Interactions

Ecological interaction network for Pseudomonas-Rhodococcus Chloronitrobenzene Coculture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Pseudomonas Partial Chloronitrobenzene Transformation

CROSS_FEEDING

Source Taxon: Pseudomonas putida HS12

Target Taxon: Rhodococcus sp. strain HS51

Metabolites: 3-chloronitrobenzene (CHEBI:82420), 4-chloronitrobenzene (CHEBI:34399)

Biological Processes:

  • aromatic compound catabolic process (GO:0019439)
  • interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:10049867 - SUPPORT (IN_VITRO)
    "NB-grown cells of P. putida HS12 were found to convert 3- and 4-CNBs"
  • PMID:10049867 - SUPPORT (IN_VITRO)
    "by partial reduction and subsequent acetylation"

Rhodococcus Intermediate Degradation

CROSS_FEEDING

Source Taxon: Rhodococcus sp. strain HS51

Target Taxon: Pseudomonas putida HS12

Biological Processes:

  • aromatic compound catabolic process (GO:0019439)
  • interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:10049867 - SUPPORT (IN_VITRO)
    "Rhodococcus sp. strain HS51, which degrades 4- and 5-chloro-2-hydroxyacetanilides"
  • PMID:10049867 - SUPPORT (IN_VITRO)
    "A degradation pathway for 3- and 4-CNBs by the two isolated strains was also proposed"

Coculture Chloronitrobenzene Mineralization

SYNTROPHY

Source Taxon: Pseudomonas putida HS12

Metabolites: 3-chloronitrobenzene (CHEBI:82420), 4-chloronitrobenzene (CHEBI:34399)

Biological Processes:

  • aromatic compound catabolic process (GO:0019439)

Evidence

  • PMID:10049867 - SUPPORT (IN_VITRO)
    "This coculture was confirmed to mineralize 3- and 4-CNBs"
  • PMID:10049867 - SUPPORT (IN_VITRO)
    "in the presence of an additional carbon source"

External Resources

Name Repository Resource ID
Exact-system primary publication - P. putida HS12 and Rhodococcus sp. HS51
Primary publication describing the exact two-member chloronitrobenzene-mineralizing coculture of P. putida HS12 and Rhodococcus sp. strain HS51. 		OTHER PMID:10049867
  • PMID:10049867 - SUPPORT (IN_VITRO)
    "was isolated and combined with P. putida HS12 to give a coculture"
OSTI bibliographic record
OSTI bibliographic entry for the same Applied and Environmental Microbiology article. 		OTHER OSTI:335354
  • PMID:10049867 - SUPPORT (IN_VITRO)
    "Degradation of chloronitrobenzenes by a coculture of Pseudomonas putida and a Rhodococcus sp"

Environmental Factors

Factor Value Unit
Chloronitrobenzene pollutants 3- and 4-chloronitrobenzene N/A
  • PMID:10049867 - SUPPORT (IN_VITRO)
    "This coculture was confirmed to mineralize 3- and 4-CNBs"
Nitrobenzene enrichment substrate nitrobenzene N/A
  • PMID:10049867 - SUPPORT (IN_VITRO)
    "analogue enrichment culture using nitrobenzene (NB) as the substrate"
Additional carbon source additional carbon source N/A
  • PMID:10049867 - SUPPORT (IN_VITRO)
    "in the presence of an additional carbon source"

Growth Media

Chloronitrobenzene mineralization coculture assay

Atmosphere AEROBIC

Composition

Component Concentration Unit CHEBI
3-chloronitrobenzene N/A N/A CHEBI:82420
4-chloronitrobenzene N/A N/A CHEBI:34399
additional carbon source N/A N/A N/A

Preparation notes: The abstract confirms 3- and 4-CNB mineralization in the presence of an additional carbon source; more specific medium formulation is not asserted here.

Evidence

PMID:10049867

"This coculture was confirmed to mineralize 3- and 4-CNBs"

PMID:10049867

"in the presence of an additional carbon source"