A defined two-member aerobic laboratory coculture for chloronitrobenzene bioremediation. Pseudomonas putida HS12 was isolated using nitrobenzene enrichment and performs the first partial reductive and acetylation steps on 3- and 4-chloronitrobenzene. Rhodococcus sp. strain HS51 degrades the chlorohydroxyacetanilide intermediates, allowing the coculture to mineralize 3- and 4-chloronitrobenzenes when an additional carbon source is supplied.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Pseudomonas putida HS12 — ontology label: Pseudomonas putida | NCBITaxon:303 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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N/A |
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| Rhodococcus sp. strain HS51 — ontology label: Rhodococcus <high G+C Gram-positive bacteria> | NCBITaxon:1827 · TaxonMech · TraitMech |
CROSS_FEEDER
PRIMARY_DEGRADER
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N/A |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Pseudomonas Partial Chloronitrobenzene Transformation
CROSS_FEEDINGThe source reports chemically identified conversion of the substrates into intermediates subsequently degraded by the partner. PARTIAL: the sequential transformation supports the reported directed coupling, not reciprocal metabolite supply or directly traced assimilation by both partners.
Source Taxon: Pseudomonas putida HS12
Target Taxon: Rhodococcus sp. strain HS51
Metabolites: 3-chloronitrobenzene (CHEBI:82420 · MediaIngredientMech · CMMMech), 4-chloronitrobenzene (CHEBI:34399 · MediaIngredientMech · CMMMech)
Biological Processes:
- interspecies interaction between organisms (GO:0044419 · PathwayMech)
The reported upstream transformation produces the intermediates that the downstream partner degrades, coupling the two steps. PARTIAL: the accessible abstract does not resolve every transfer or assimilation step.
Evidence
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PMID:10049867 - SUPPORT (IN_VITRO)"NB-grown cells of P. putida HS12 were found to convert 3- and 4-CNBs"
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PMID:10049867 - SUPPORT (IN_VITRO)"by partial reduction and subsequent acetylation"
Rhodococcus Intermediate Degradation
Rhodococcus degrades the intermediates generated by Pseudomonas in the proposed two-member pathway. This is the downstream transformation step, not evidence of Rhodococcus supplying a metabolite back to Pseudomonas.
Participating Taxa: Pseudomonas putida HS12, Rhodococcus sp. strain HS51
Biological Processes:
- interspecies interaction between organisms (GO:0044419 · PathwayMech)
The reported downstream intermediate degradation contributes to the proposed two-strain pathway and conditional coculture mineralization. PARTIAL: the full pathway and carbon-balance measurements were not independently inspected.
Evidence
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PMID:10049867 - SUPPORT (IN_VITRO)"Rhodococcus sp. strain HS51, which degrades 4- and 5-chloro-2-hydroxyacetanilides"
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PMID:10049867 - SUPPORT (IN_VITRO)"A degradation pathway for 3- and 4-CNBs by the two isolated strains was also proposed"
Coculture Chloronitrobenzene Mineralization
The primary abstract reports coculture mineralization in the presence of an additional carbon source. Preserve that conditional result. The endpoint is not itself evidence that removal of a partner product makes the upstream metabolism favourable, and a complete carbon balance was not independently audited.
Participating Taxa: Pseudomonas putida HS12, Rhodococcus sp. strain HS51
Metabolites: 3-chloronitrobenzene (CHEBI:82420 · MediaIngredientMech · CMMMech), 4-chloronitrobenzene (CHEBI:34399 · MediaIngredientMech · CMMMech)
Evidence
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PMID:10049867 - SUPPORT (IN_VITRO)"This coculture was confirmed to mineralize 3- and 4-CNBs"
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PMID:10049867 - SUPPORT (IN_VITRO)"in the presence of an additional carbon source"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN What evidence resolves transfer direction and the conditional mineralization pathway?
The sequential transformations and reported mineralization are preserved. The reverse-feeding label on the intermediate-degradation node was removed without reversing either graph arrow. Only the primary abstract was retrieved: XML failed after bounded retries, the article page presented a browser challenge and the open-access metadata request returned404. Missing full methods are not evidence that controls were absent. SYNTROPHY does not require obligacy in the local schema; the endpoint label is removed because pathway completion alone does not demonstrate product-removal feedback on the upstream metabolism.
Attaches to: ecological_interactions#Pseudomonas Partial Chloronitrobenzene Transformation, ecological_interactions#Rhodococcus Intermediate Degradation, ecological_interactions#Coculture Chloronitrobenzene Mineralization
2 evidence item(s)
Rhodococcus sp. strain HS51, which degrades 4- and 5-chloro-2-hydroxyacetanilides
Supports HS51 as the organism degrading P. putida-derived intermediates.
This coculture was confirmed to mineralize 3- and 4-CNBs
Directly supports the community-level mineralization claim.
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Exact-system primary publication - P. putida HS12 and Rhodococcus sp. HS51
Primary publication describing the exact two-member chloronitrobenzene-mineralizing coculture of P. putida HS12 and Rhodococcus sp. strain HS51. |
OTHER | PMID:10049867 |
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OSTI bibliographic record
OSTI bibliographic entry for the same Applied and Environmental Microbiology article. |
OTHER | OSTI:335354 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Chloronitrobenzene pollutants | 3- and 4-chloronitrobenzene | N/A |
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| Nitrobenzene enrichment substrate | nitrobenzene | N/A |
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| Additional carbon source | additional carbon source | N/A |
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Growth Media
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