A defined three-fungus starter for controlled ripe Pu'er tea pile fermentation. Yan et al. 2026 inoculated sun-dried green tea leaves with a patented Saccharomyces cerevisiae, Rhizopus arrhizus, and Aspergillus niger starter at a 2:1:2 weight ratio, then integrated sensory evaluation, physicochemical measurements, volatile and non-volatile metabolomics, and metagenomic sequencing across six fermentation stages to profile the emergence of a floral-fruity aroma.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
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| Saccharomyces cerevisiae | NCBITaxon:4932 · TaxonMech · TraitMech | N/A | |
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| Rhizopus arrhizus | NCBITaxon:64495 · TaxonMech · TraitMech | N/A | |
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| Aspergillus niger | NCBITaxon:5061 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Floral-fruity aroma emergence under three-fungus inoculation
Pu'er tea inoculated with the three-fungus starter was associated with a distinct floral-fruity aroma, while combined ROAV and VIP analyses prioritized geraniol, phenylethyl alcohol, trans-β-ionone, and 1-octen-3-ol as aroma-active volatiles.
Biological Processes:
- fermentation (GO:0006113 · PathwayMech)
Evidence
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PMID:42331835 - SUPPORT (IN_VITRO)"Inoculation was associated with a distinct floral-fruity aroma."
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PMID:42331835 - SUPPORT (IN_VITRO)"geraniol, phenylethyl alcohol, trans-β-ionone, and 1-octen-3-ol also exhibited VIP > 1"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which culture-collection accessions or formal strain designations correspond to the patented S. cerevisiae, R. arrhizus, and A. niger starter components used for the 2:1:2 ripe Pu'er tea fungal SynCom?
PMID:42331835 names three species and lists patent identifiers for each starter component, but the paper does not expose culture-collection accessions or formal strain names that would make the components independently recoverable.
Attaches to: taxonomy#Saccharomyces cerevisiae, taxonomy#Rhizopus arrhizus, taxonomy#Aspergillus niger
External Resources
| Name | Repository | Resource ID |
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PubMed - Pu'er fungal floral-fruity SynCom
PubMed record for the npj Science of Food article describing multi-omics profiling of floral-fruity aroma formation in Pu'er tea inoculated with a synthetic fungal community. |
OTHER | PMID:42331835 |
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DOI - Pu'er fungal floral-fruity SynCom
DOI landing page for the npj Science of Food article describing a three-fungus synthetic starter for Pu'er tea fermentation. |
OTHER | doi:10.1038/s41538-026-00945-x |
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Growth Media
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