A defined two-member Saccharomyces cerevisiae coculture that partitions glucose and arabinose fermentation between specialist strains. ACE2 deletion gives the arabinose specialist a multicellular, fast-sedimenting phenotype, allowing selective biomass retention during sequential-batch cultivation and faster mixed-sugar conversion.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| glucose-specialist Saccharomyces cerevisiae strain — ontology label: Saccharomyces cerevisiae | NCBITaxon:4932 · TaxonMech · TraitMech | N/A | |
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| sedimenting arabinose-specialist Saccharomyces cerevisiae strain — ontology label: Saccharomyces cerevisiae | NCBITaxon:4932 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Glucose-Arabinose Substrate Specialization
NICHE_PARTITIONINGThe two yeast specialists partition glucose and arabinose use in the tested defined-medium coculture. The slower arabinose conversion is the bottleneck for complete mixed-sugar depletion. This substrate specialization does not itself demonstrate reciprocal facilitation or explain the improvement obtained with selective biomass retention.
Metabolites: glucose (CHEBI:17234 · MediaIngredientMech · CMMMech)
Biological Processes:
- fermentation (GO:0006113 · PathwayMech)
PARTIAL: separating sugar use makes the slow arabinose specialist a target for selective retention. The faster overall conversion requires the sedimenting phenotype and repeated settling treatment; specialization alone was not shown to cause the batch-time improvement.
Evidence
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PMID:42461012 - SUPPORT (IN_VITRO)"Co-cultures of a glucose-specialist S. cerevisiae strain with the sedimenting arabinose specialist on a glucose-arabinose mixture"
Selective Sedimentation Accelerates Mixed-Sugar Conversion
Under the reported sequential-batch settling regime, cocultures containing the sedimenting arabinose specialist had a 29% shorter batch time than those with its non-sedimenting counterpart. The authors attribute the improvement to selective biomass retention. This is a process-level conversion outcome, not a separate niche-partitioning interaction or evidence of faster intrinsic growth.
Metabolites: glucose (CHEBI:17234 · MediaIngredientMech · CMMMech)
Biological Processes:
- fermentation (GO:0006113 · PathwayMech)
Longer settling increased retained biomass and accelerated arabinose conversion, bringing its depletion closer to glucose depletion in the tested sequential-batch coculture. This supports the retention-to-timing direction under the reported treatment, not a universal relation between settling and performance.
Evidence
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PMID:42461012 - SUPPORT (IN_VITRO)"showed a 29% shorter batch time than corresponding co-cultures with the non-sedimenting arabinose specialist"
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PMID:42461012 - SUPPORT (IN_VITRO)"Increasing the biomass settling period to 1 h further reduced the batch time to 8.1 ± 0.1 h and resulted in near-simultaneous depletion of both sugars"
Near-Simultaneous Mixed-Sugar Depletion
The reported longer-settling treatment yielded near-simultaneous glucose and arabinose depletion and a batch time of 8.1 hours. This is the measured timing outcome of the tested two-specialist process, distinct from the underlying substrate partitioning and not proof of an interstrain benefit.
Metabolites: glucose (CHEBI:17234 · MediaIngredientMech · CMMMech)
Biological Processes:
- fermentation (GO:0006113 · PathwayMech)
Evidence
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PMID:42461012 - SUPPORT (IN_VITRO)"Increasing the biomass settling period to 1 h further reduced the batch time to 8.1 ± 0.1 h and resulted in near-simultaneous depletion of both sugars"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN How far does the selective-retention benefit transfer beyond the tested defined-medium process?
The source supports the specialist coculture comparison and settling-associated conversion benefit, but industrial transfer remains conditional. Retain the two existing directions with an explicit limitation on specialization alone as a cause. Do not equate faster batch conversion with faster intrinsic growth or mutualism.
Attaches to: ecological_interactions#Glucose-Arabinose Substrate Specialization, ecological_interactions#Selective Sedimentation Accelerates Mixed-Sugar Conversion, ecological_interactions#Near-Simultaneous Mixed-Sugar Depletion
1 evidence item(s)
if selective sedimentation of specific slow-fermenting strains can be implemented under relevant process conditions
The authors make process transfer conditional rather than establishing universal performance.
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Primary publication - sedimenting glucose-arabinose specialist coculture
Primary study of the exact two-member specialist coculture curated in this record. |
OTHER | PMID:42461012 |
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Related exact-species publication - glucose-xylose specialist yeast consortium
A separate two-strain S. cerevisiae specialist consortium with the same species-only composition but different engineered strains and glucose-xylose substrates. |
OTHER | PMID:38278783 |
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Related exact-species publication - three-strain glucose-xylose-arabinose consortium
A three-strain S. cerevisiae specialist consortium with the same species-only composition but an additional xylose-specialist member. |
OTHER | PMID:30010916 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Mixed-sugar substrate | glucose 10 g/L and arabinose 10 g/L | N/A |
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| Biomass settling period | 30 minutes or 1 hour | N/A |
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| Atmosphere | anaerobic | N/A |
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Growth Media
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