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SkinCom Synthetic Skin Community

Community ID CommunityMech:000125
Ecological State ENGINEERED
Environment skin-associated microbial habitat — ontology label: skin of body
UBERON:0002097 · HabitatMech ↗

A standardized synthetic skin microbial community for reproducible in vitro and in vivo studies of skin-associated microbe-metabolite interactions.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
skin bacterial SynCom members — ontology label: Bacteria NCBITaxon:2 · TaxonMech · TraitMech N/A
  • PMID:39111313 - SUPPORT (IN_VIVO)
    "SkinCom represents a valuable, standardized tool for investigating microbe-metabolite interactions"

Ecological Interactions

The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.

Ecological interaction network for SkinCom Synthetic Skin Community Bipartite graph where circles represent taxa and non-circular symbols represent interactions. Rare or untyped interactions share the Other symbol; exact curated types remain in the interaction details.
Taxon
Other

SkinCom Medium-Associated Diversity Difference

The compared SkinCom medium conditions differed in diversity and composition. All intended isolates were detected in both conditions. This is a community-composition observation, not evidence that the diversity difference caused C. acnes presence or identified an interspecies mechanism.

Biological Processes:

Evidence

  • PMID:39111313 - SUPPORT (IN_VITRO)
    "sequencing data confirmed the presence of all intended isolates within both sets of SkinComs"
  • PMID:39111313 - SUPPORT (IN_VITRO)
    "significant compositional differences between the 0.1× and 1× BHI communities"

Community-Associated Cutibacterium Detection in Oxic SkinCom

C. acnes was detected in the oxic mixed community; the authors interpret the metagenomic result as proliferation. HYPOTHESIZED: oxygen consumption by aerobic members may explain this association. The reported comparison does not isolate that mechanism or identify a particular facilitating partner, and is not represented as demonstrated colonization facilitation.

Biological Processes:

Evidence

  • PMID:39111313 - PARTIAL (IN_VITRO)
    "metagenomic results confirmed the ability of C. acnes to proliferate in an oxic setting, likely facilitated by oxygen consumption by aerobic members of the SkinCom"

Cosmetic Chemical Response Concordance

The study reports an average 66% agreement in the direction of differential-abundance changes between SkinCom and a small human pilot (13 participants). Only one human comparison was statistically significant. Directional concordance is not replication of significant effects, clinical efficacy, or evidence that the in-vitro and human results are interchangeable.

Biological Processes:

Evidence

  • PMID:39111313 - SUPPORT (IN_VITRO)
    "66% of the responses to common cosmetic chemicals in vitro were in agreement with a human trial"
  • PMID:39111313 - SUPPORT (IN_VIVO)
    "Note that the only statistically significant change observed was for S. epidermidis in the day 7 SLES cohort. No other changes were statistically significant."

Knowledge gaps & discussions (1)

KNOWLEDGE_GAP OPEN How strongly do the reported SkinCom observations establish mediation and transferability to human skin?

The primary abstract, results, discussion and limitations were read. They support community-associated detection, a proposed oxygen-consumption explanation and directional response agreement. They do not isolate the proposed mediator or make the models interchangeable. Mechanistic attribution and external validity require independent evidence. No new growth, colonization or infection procedures are proposed; existing non-graph cultivation text is outside this graph review.

Attaches to: ecological_interactions#SkinCom Medium-Associated Diversity Difference, ecological_interactions#Community-Associated Cutibacterium Detection in Oxic SkinCom, ecological_interactions#Cosmetic Chemical Response Concordance

1 evidence item(s)
OTHER SUPPORT PMID:39111313

Due to such different experimental designs, results cannot be used interchangeably.

The authors explicitly limit cross-model equivalence.

Environmental Factors

Factor Value Unit
standardized skin model conditions SkinCom was evaluated in vitro and after application to dorsal skin of mice. N/A
  • PMID:39111313 - SUPPORT (IN_VIVO)
    "SkinCom represents a valuable, standardized tool for investigating microbe-metabolite interactions"

Growth Media

brain-heart infusion (BHI)

Temperature 37 °C

Preparation notes: Strains cultured in 1X BHI at 37°C without shaking; obligate anaerobes (e.g. C. acnes) in cysteine-reduced medium in Hungate tubes. Aerobic and anaerobic members grown separately, anaerobic incubation in a vinyl anaerobic chamber (N2/CO2/H2 85%/10%/5%). Oxic and anoxic growth monitored for 144 and 140 h respectively; inoculum diluted to OD600 0.065 and arrayed into 96-well plates.

Evidence

PMID:39111313

"Individual strains were cultured in sterile BHI broth at 37°C, without shaking."

PMID:39111313

"Under anaerobic conditions, plates were incubated in a vinyl anaerobic chamber (Coy Lab Products) with an atmosphere of N2/CO2/H2 (85%/10%/5%)."

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: skin-associated microbial habitat ↗ TaxonMech: skin bacterial SynCom members ↗ TraitMech: skin bacterial SynCom members ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗