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Streptomyces A2-A5-A11-M7 Pesticide-Bioremediation Consortium

Community ID CommunityMech:000335
Ecological State ENGINEERED
Environment pesticide-bioremediation laboratory and soil systems
ENVO:01001405
Modeled Environment pesticide-contaminated soil
ENVO:00002116

A repeatedly studied defined consortium of four Argentine Streptomyces soil and sediment isolates: A2, A5, A11, and M7. The unchanged composition has been evaluated across liquid cultures, immobilized cultures, soil slurries, microcosms, and mesocosms for lindane and other chlorinated pesticides, and most recently for atrazine removal and detoxification.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Streptomyces sp. A2 NCBITaxon:1931 N/A
  • PMID:41678860 - SUPPORT (IN_VITRO)
    "C1, constituted by Streptomyces sp. A2, A5, A11, and M7"
Streptomyces sp. A5 NCBITaxon:1931 N/A
  • PMID:41678860 - SUPPORT (IN_VITRO)
    "C1, constituted by Streptomyces sp. A2, A5, A11, and M7"
Streptomyces sp. A11 NCBITaxon:1931 N/A
  • PMID:41678860 - SUPPORT (IN_VITRO)
    "C1, constituted by Streptomyces sp. A2, A5, A11, and M7"
Streptomyces sp. M7 NCBITaxon:1931 N/A
  • PMID:41678860 - SUPPORT (IN_VITRO)
    "C1, constituted by Streptomyces sp. A2, A5, A11, and M7"

Ecological Interactions

Ecological interaction network for Streptomyces A2-A5-A11-M7 Pesticide-Bioremediation Consortium Bipartite graph where circle nodes represent taxa and each ecological interaction is drawn as a distinct non-circular symbol, with colour repeating the same distinction (mutualism).
Taxon
Mutualism

Non-antagonistic community-level growth enhancement

MUTUALISM

Evidence

  • PMID:23865051 - SUPPORT (IN_VITRO)
    "Results revealed that the strains did not have any antagonistic effects and showed tolerance against the pesticides mixture. In fact, the growth of mixed cultures was significantly higher than in pure cultures."
  • PMID:41678860 - SUPPORT (IN_VITRO)
    "revealing their metabolic versatility and potential cooperative work, fundamental for the degradation of xenobiotic compounds."

External Resources

Name Repository Resource ID
2011 defined lindane consortium study
Original selection study for lindane degradation by the exact A2-A5-A11-M7 composition. 		OTHER doi:10.1007/s11270-011-0818-5
2012 immobilized lindane cultures study
Compared free and immobilized cultures and reuse of the exact four-strain consortium. 		OTHER PMID:22840534
  • PMID:22840534 - SUPPORT (IN_VITRO)
    "consortium I, consisting of the four immobilized strains together"
2013 chlorpyrifos-pentachlorophenol mixture study
Evaluated the exact consortium as one of several pure and mixed cultures against a two-pesticide mixture. 		OTHER PMID:23865051
  • PMID:23865051 - SUPPORT (IN_VITRO)
    "The first mixed culture was an Argentinian microbial consortium (Streptomyces spp. A2-A5-A11-M7)"
2014 immobilized soil-slurry study
Tested the exact consortium in cloth sachets at different inoculum, lindane, and slurry concentrations. 		OTHER doi:10.1016/j.ibiod.2014.05.013
2015 acclimation and lindane-biodegradation study
Tested acclimated and non-acclimated free and immobilized forms of the exact consortium. 		OTHER doi:10.1016/j.procbio.2015.08.014
2017 mixed-organochlorine pesticide study
Evaluated simultaneous lindane, chlordane, and methoxychlor removal in liquid, soil, and slurry systems. 		OTHER doi:10.1016/j.chemosphere.2017.01.044
2019 lindane soil-texture study
Compared bioaugmentation outcomes in sandy, silty-loam, and clayey soils. 		OTHER doi:10.1007/s42729-018-0003-7
2020 sugarcane-bagasse biostimulation study
Coupled the exact consortium with sugarcane-bagasse biostimulation in different soils. 		OTHER PMID:31430718
  • PMID:31430718 - SUPPORT (IN_VITRO)
    "the actinobacteria consortium composed of Streptomyces sp. A2, A5, A11 and M7"
2020 sugarcane-filter-cake biostimulation study
Optimized soil bioaugmentation with the exact consortium plus sugarcane filter cake. 		OTHER doi:10.1016/j.ecoenv.2019.110143
2020 microcosm-to-mesocosm scaling study
Scaled bioaugmentation with the exact consortium and sugarcane filter cake from microcosms to mesocosms. 		OTHER doi:10.1016/j.jenvman.2020.111309
2026 atrazine-removal study
Tested the exact consortium as C1 for atrazine growth, removal, gene detection, and phytotoxicity reversal. 		OTHER PMID:41678860
  • PMID:41678860 - SUPPORT (IN_VITRO)
    "C1, constituted by Streptomyces sp. A2, A5, A11, and M7"

Environmental Factors

Factor Value Unit
Atrazine concentration 25 and 50 mg/L
  • PMID:41678860 - SUPPORT (IN_VITRO)
    "four actinomycetota consortia (C1, C2, C3, and C4) were tested about their ability to grow and remove 25 and 50 mg L-1 of atrazine as the only carbon source."
Atrazine nutrient role sole carbon source or sole nitrogen source N/A
  • PMID:41678860 - SUPPORT (IN_VITRO)
    "no statistically significant differences (p > 0.05) were observed between the growth values detected with atrazine as a carbon (2.98 +/- 0.13 g L-1) or nitrogen source (2.88 +/- 0.18 g L-1)."
Atrazine phytotoxicity reversal strongest with atrazine as carbon source N/A
  • PMID:41678860 - SUPPORT (IN_VITRO)
    "Phytotoxicity tests demonstrated the ability of C1 to reverse the herbicide toxicity, especially with atrazine as a carbon source."
Lindane removal in immobilized mixed culture 81 percent
  • PMID:22840534 - SUPPORT (IN_VITRO)
    "reaching a maximum removal of 81% after 96 h for consortium I, consisting of the four immobilized strains together."

Growth Media

Tryptic soy broth inoculum medium

Temperature 30 degrees Celsius

Composition

Component Concentration Unit CHEBI
trypticase 15.0 g/L N/A
soy peptone 3.0 g/L N/A
sodium chloride 5.0 g/L N/A
dipotassium hydrogen phosphate 2.5 g/L N/A
glucose 2.5 g/L N/A

Preparation notes: Each Streptomyces strain was cultivated separately in TSB before consortium assays; cultures were shaken at 200 rpm.

Evidence

PMID:23865051

"Tryptic Soy Broth (TSB), containing (g L-1): trypticase, 15.0; soy peptone, 3.0; NaCl, 5.0; K2HPO4, 2.5; glucose, 2.5"

PMID:23865051

"Cultures were incubated at 30 C for 72 h on a rotary shaker at 200 rpm"

Chlorpyrifos-pentachlorophenol minimal-medium consortium assay

Temperature 30 degrees Celsius

Composition

Component Concentration Unit CHEBI
chlorpyrifos 1.66 mg/L CHEBI:34631
pentachlorophenol 1.66 mg/L CHEBI:17642

Preparation notes: The four-strain consortium was tested as free cells in 30 mL minimal medium containing both pesticides and shaken at 200 rpm.

Evidence

PMID:23865051

"2 g L-1 of biomass (wet weight) of the microbial consortia Streptomyces spp. A2-A5-A11-M7"

PMID:23865051

"containing 30 mL MM spiked with the pesticides mixture (1.66 mg L-1 of each one). Cultures were incubated at 30 C for 72 h on a rotary shaker at 200 rpm"