A deep subsurface (1.4 km) aquifer microbial community perturbed by injection of 150 tons of supercritical CO2 (scCO2) in a geosequestration experiment. Detailed genome-resolved metagenomics resolved the native carboxydotrophic Carboxydocella, a thermophilic CO-utilizing anaerobe whose relative abundance decreased post-scCO2 injection. Analysis of its carbon monoxide dehydrogenase (CODH) gene before and after the experiment documented a switch in CO-oxidation potential by Carboxydocella, with implications for subsurface flow of carbon and electrons from oxidation of the metabolic intermediate CO.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Carboxydocella (thermophilic CO-utilizing anaerobe) — ontology label: Carboxydocella | NCBITaxon:178898 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
scCO2 Injection Reshapes Subsurface Community
The source reports community-structure changes following supercritical carbon dioxide injection into the deep aquifer. Retain the field perturbation as abiotic input context; the accessible abstract does not isolate the mechanism of the community response.
Metabolites: carbon dioxide (CHEBI:16526 · MediaIngredientMech · CMMMech)
HYPOTHESIZED/PARTIAL: the injected supercritical carbon dioxide is the upstream field perturbation proposed to alter conditions underlying the subsequent Carboxydocella relative-abundance decrease. The temporal association does not establish that aggregate community restructuring itself caused the decline or isolate the physiological mediator.
Evidence
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PMID:32633030 - SUPPORT (IN_VIVO)"changes to the microbial community structure of a deep aquifer (1.4 km) receiving 150 tons of injected supercritical CO2"
Post-Injection Carboxydocella Decline
Carboxydocella decreased in relative abundance after the field injection. Relative abundance does not establish absolute cell loss, mortality or a measured fitness decrease, and the accessible abstract does not isolate the mechanism.
Metabolites: carbon monoxide (CHEBI:17245 · MediaIngredientMech · CMMMech)
Evidence
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PMID:32633030 - SUPPORT (IN_VIVO)"the thermophilic CO-utilizing anaerobe Carboxydocella, which decreased in relative abundance post-scCO2 injection "
CODH-Associated CO-Oxidation Potential
The source infers a before/after difference in Carboxydocella CO-oxidation potential from CODH gene analysis. Preserve this genomic interpretation, not a demonstrated physiological switch or a measured oxidation rate.
Metabolites: carbon monoxide (CHEBI:17245 · MediaIngredientMech · CMMMech)
HYPOTHESIZED/PARTIAL: a change in encoded CO-oxidation potential could alter carbon/electron flow through carbon monoxide. The source discusses this implication; the accessible abstract does not report a measured flux or isolate CODH variation as the causal mediator.
Evidence
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PMID:32633030 - SUPPORT (COMPUTATIONAL)"a switch in CO-oxidation potential by Carboxydocella through analysis of its carbon monoxide dehydrogenase (CODH) gene before and after the geosequestration experiment "
Potential Subsurface Carbon-Electron Flow Through CO
HYPOTHESIZED/PARTIAL: the source discusses potential impacts on subsurface carbon and electron flow through carbon monoxide. This is an implication of the genomic comparison, not measured community flux or demonstrated metabolite exchange.
Metabolites: carbon monoxide (CHEBI:17245 · MediaIngredientMech · CMMMech)
Evidence
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PMID:32633030 - PARTIAL (COMPUTATIONAL)"subsurface flow of carbon and electrons from oxidation of the metabolic intermediate carbon monoxide (CO) "
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which field responses and genomic interpretations establish absolute population change or functional flux?
Keep the field association and relative-abundance observation separate from gene-based functional potential. Remove the isolated genome-resolution workflow node, preserving the prior analysis-edge repair. The two remaining directions are hypotheses/partial interpretations rather than isolated causal mediators. The primary abstract was read; a failed publisher request is not proof that full text is absent elsewhere.
Attaches to: ecological_interactions#scCO2 Injection Reshapes Subsurface Community, ecological_interactions#Post-Injection Carboxydocella Decline, ecological_interactions#CODH-Associated CO-Oxidation Potential, ecological_interactions#Potential Subsurface Carbon-Electron Flow Through CO
1 evidence item(s)
subsurface flow of carbon and electrons from oxidation of the metabolic intermediate carbon monoxide (CO)
Supports the source discussion of potential carbon/electron-flow impacts; the surrounding abstract explicitly qualifies these as potential.
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Primary publication for the Carboxydocella aquifer community
PubMed record for the Mu et al. 2020 Environmental Microbiology Reports paper. |
OTHER | PMID:32633030 |
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DOI landing page
DOI link to the Environmental Microbiology Reports paper. |
OTHER | doi:10.1111/1758-2229.12868 |
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Supercritical CO2 injection | 150 tons of injected scCO2 | N/A |
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| Aquifer depth | 1.4 km below surface | N/A |
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