A 10-member synthetic chicken intestinal community that promotes gut homeostasis and anti-Salmonella immunity through segmented filamentous bacteria establishment.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| chicken intestinal SynComBac10 members — ontology label: Bacteria | NCBITaxon:2 · TaxonMech · TraitMech |
CROSS_FEEDER
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N/A |
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| segmented filamentous bacteria — ontology label: Candidatus Neoarthromitus | NCBITaxon:49082 · TaxonMech · TraitMech |
CROSS_FEEDER
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N/A |
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| Salmonella enterica serovar Typhimurium — ontology label: Salmonella enterica subsp. enterica serovar Typhimurium | NCBITaxon:90371 · TaxonMech · TraitMech | N/A | |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Predicted SynCom-to-SFB metabolite exchange
CROSS_FEEDINGHYPOTHESIZED/PARTIAL: iNAP 2.0 predicted potential metabolite exchange from SynComBac10 to segmented filamentous bacteria, including the reported siderophore candidate. Culture-supernatant metabolomics corroborated production of a subset of predicted metabolites, but production is not measured transfer to SFB in the intestine. The source proposes cross-feeding as an explanation for SFB establishment and acknowledges alternative community effects.
Participating Taxa: chicken intestinal SynComBac10 members, segmented filamentous bacteria
Metabolites: staphyloferrin B (CHEBI:134612 · MediaIngredientMech · CMMMech)
Biological Processes:
- multi-organism process (GO:0044419 · PathwayMech)
HYPOTHESIZED/PARTIAL: predicted nutrient provision may help account for the observed SFB establishment upstream of Th17 induction. The study supports SFB-associated immune induction, but does not establish the predicted exchange as its necessary upstream mediator.
Evidence
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PMID:40266232 - PARTIAL (COMPUTATIONAL)"staphyloferrin B, a siderophore, could be provided from six SynComBac10 members (L. reuteri CML393, B. velezensis CML396, E. faecium CML291, M. funiformis CML154, R. lituseburensis CML137, and E. sp. CML138) to SFB"
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PMID:40266232 - SUPPORT (IN_VITRO)"we performed metabolomic profiling of the bacterial culture supernatants, and ultimately, a total of 18 metabolites were confirmed"
SFB-Driven Th17 Immune Priming
SFB-associated intervention increased intestinal Th17 cells, supported by cellular profiling and flow cytometry, alongside the Th17 increase in SynComBac10-treated chickens. This is host immune induction, not a colonization-facilitation interaction. The specifically Th17 observation should not be represented only as regulation of innate immunity.
Participating Taxa: segmented filamentous bacteria
HYPOTHESIZED/PARTIAL: SFB-associated Th17 responses are proposed to contribute to the protective host outcomes after SynComBac10 exposure. The source explicitly leaves other immune and non-immune contributions open, so this is not an exclusive or experimentally isolated mediation claim.
Evidence
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PMID:40266232 - SUPPORT (IN_VIVO)"SFB), which in turn induced Th17 cell-mediated immune responses, thereby conferring resistance to Salmonella infection"
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PMID:40266232 - SUPPORT (IN_VIVO)"The induction of Th17 cells after SFB spore inoculation was also evidenced by the flow cytometry results"
Host protection after SynComBac10 exposure
SynComBac10-treated chickens showed improved host growth, tissue and serum outcome measures in the reported Salmonella comparison. SFB-associated immune responses are a proposed contributor, not the sole established cause of protection. These host endpoints do not demonstrate reciprocal-negative competition between SFB and Salmonella, pathogen eradication, or a universal effect across hosts.
Participating Taxa: segmented filamentous bacteria, Salmonella enterica serovar Typhimurium
Biological Processes:
- defense response to bacterium (GO:0042742 · PathwayMech)
Evidence
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PMID:40266232 - PARTIAL (IN_VIVO)"SFB-enriched SynComBac10 chickens were highly resistant to Salmonella infection due to the SFB-mediated immune responses"
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PMID:40266232 - SUPPORT (IN_VIVO)"the anti-Salmonella effect of SynComBac10 treatment may not be solely attributed to its induction of SFB colonization."
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN How much of the protective host outcome is mediated by predicted metabolite transfer and SFB-associated Th17 responses?
Retain the measured metabolite-production, immune and host-protection findings. The source does not establish recipient uptake of the predicted metabolites or exclusive mediation through SFB. This record-level repair adds no procedures, strain designs, pathogen enhancement mechanisms or optimization advice.
Attaches to: ecological_interactions#Predicted SynCom-to-SFB metabolite exchange, ecological_interactions#SFB-Driven Th17 Immune Priming, ecological_interactions#Host protection after SynComBac10 exposure
1 evidence item(s)
the anti-Salmonella effect of SynComBac10 treatment may not be solely attributed to its induction of SFB colonization.
States the source limitation on exclusive SFB mediation.
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| early-life gut SynCom exposure | Chickens were exposed early in life to SynComBac10 and evaluated for gut maturation and Salmonella resistance. | N/A |
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Growth Media
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