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Synechococcus-Halomonas Light-Driven PHB Coculture

Community ID CommunityMech:000198
Ecological State ENGINEERED
Environment illuminated laboratory photobioreactor coculture
ENVO:01001405

A synthetic, light-driven two-member phototroph-heterotroph consortium pairing sucrose-exporting Synechococcus elongatus PCC 7942 cscB with the halotolerant polyhydroxybutyrate producer Halomonas boliviensis LC1. The cyanobacterium fixes carbon dioxide under illumination and exports sucrose, while H. boliviensis consumes the cyanobacterial sucrose and accumulates the bioplastic precursor polyhydroxybutyrate. The system is relevant to DOE carbon utilization and bioproduct goals because it couples photosynthetic carbon fixation to heterotrophic biopolymer production in a modular synthetic coculture.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Synechococcus elongatus PCC 7942 cscB NCBITaxon:32046
PRIMARY_PRODUCER
DOMINANT
  • PMID:29061492 - SUPPORT (IN_VITRO)
    "S. elongatus CscB"
  • PMID:29061492 - SUPPORT (IN_VITRO)
    "sucrose secreted by S. elongatus CscB"
Halomonas boliviensis LC1 NCBITaxon:1072583
CROSS_FEEDER PRIMARY_DEGRADER
COMMON
  • PMID:29061492 - SUPPORT (IN_VITRO)
    "Halomonas boliviensis"
  • PMID:29061492 - SUPPORT (IN_VITRO)
    "natural producer of the bioplastic precursor, PHB"

Ecological Interactions

Ecological interaction network for Synechococcus-Halomonas Light-Driven PHB Coculture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Photosynthetic Sucrose Transfer To Halomonas

CROSS_FEEDING

Source Taxon: Synechococcus elongatus PCC 7942 cscB

Target Taxon: Halomonas boliviensis LC1

Metabolites: carbon dioxide (CHEBI:16526), sucrose (CHEBI:17992)

Biological Processes:

Evidence

  • PMID:29061492 - SUPPORT (IN_VITRO)
    "sucrose secreted by S. elongatus CscB directly supports the bacterium Halomonas boliviensis"
  • PMID:29061492 - SUPPORT (IN_VITRO)
    "photoautotroph/chemoheterotroph consortial design"

Light-Driven Biopolymer Production

CROSS_FEEDING

Source Taxon: Synechococcus elongatus PCC 7942 cscB

Target Taxon: Halomonas boliviensis LC1

Metabolites: sucrose (CHEBI:17992)

Biological Processes:

Evidence

  • PMID:29061492 - SUPPORT (IN_VITRO)
    "stable polyhydroxybutyrate production"
  • PMID:29061492 - SUPPORT (IN_VITRO)
    "improved synthetic photoautotroph/chemoheterotroph consortial design"

External Resources

Name Repository Resource ID
Exact-system primary publication - Synechococcus/Halomonas PHB coculture
Primary publication reporting the S. elongatus cscB and H. boliviensis light-driven PHB-producing synthetic coculture. 		OTHER doi:10.1016/j.ymben.2017.10.009
  • PMID:29061492 - SUPPORT (IN_VITRO)
    "A synthetic, light-driven consortium of cyanobacteria and heterotrophic bacteria enables stable polyhydroxybutyrate production"

Environmental Factors

Factor Value Unit
Illumination light-driven culture N/A
No external organic carbon input cyanobacterial sucrose as organic carbon source N/A
  • PMID:29061492 - SUPPORT (IN_VITRO)
    "sucrose secreted by S. elongatus CscB directly supports the bacterium Halomonas boliviensis"

Growth Media

Light-driven Synechococcus-Halomonas coculture

Atmosphere AEROBIC

Composition

Component Concentration Unit CHEBI
carbon dioxide N/A N/A CHEBI:16526
sucrose N/A N/A CHEBI:17992

Preparation notes: The curated source supports a synthetic light-driven coculture and alginate hydrogel encapsulation; this record does not assert the full salts, induction, or encapsulation protocol.

Evidence

PMID:29061492

"alginate encapsulation of S. elongatus CscB"

PMID:29061492

"sucrose secreted by S. elongatus CscB directly supports the bacterium Halomonas boliviensis"