A synthetic, light-driven two-species coculture pairing engineered Synechococcus elongatus PCC 7942 cscB with engineered Pseudomonas putida strains that consume cyanobacterial sucrose. The cyanobacterium fixes CO2 and exports sucrose under salt/IPTG induction, while P. putida converts the transferred carbon into polyhydroxyalkanoates (PHA) and, in a related engineered implementation, transforms the industrial pollutant 2,4-dinitrotoluene. This platform is relevant to DOE carbon utilization, bioproduct formation, and contaminant bioremediation because it couples photosynthetic CO2 fixation to heterotrophic biocatalysis in a controlled laboratory community.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
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| Synechococcus elongatus PCC 7942 cscB | NCBITaxon:32046 |
PRIMARY_PRODUCER
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DOMINANT |
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| Pseudomonas putida engineered sucrose-utilizing partner | NCBITaxon:303 |
CROSS_FEEDER
PRIMARY_DEGRADER
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ABUNDANT |
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Ecological Interactions
Photosynthetic Sucrose Cross-Feeding
CROSS_FEEDINGSource Taxon: Synechococcus elongatus PCC 7942 cscB
Target Taxon: Pseudomonas putida engineered sucrose-utilizing partner
Metabolites: carbon dioxide (CHEBI:16526), sucrose (CHEBI:17992)
Biological Processes:
- photosynthesis (GO:0015979)
- sucrose biosynthetic process (GO:0005986)
Evidence
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PMID:28814973 - SUPPORT (IN_VITRO)"this sugar serves as C-source for Pseudomonas putida cscAB"
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PMID:32064751 - SUPPORT (IN_VITRO)"cyanobacteria fix CO2 through photosynthetic metabolism and secrete sufficient carbohydrates"
Heterotrophic PHA Accumulation
CROSS_FEEDINGSource Taxon: Pseudomonas putida engineered sucrose-utilizing partner
Metabolites: sucrose (CHEBI:17992), poly(3-hydroxybutyrate) (CHEBI:53389)
Biological Processes:
- sucrose catabolic process (GO:0005987)
Evidence
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PMID:28814973 - SUPPORT (IN_VITRO)"maximal PHA production rate of 23.8"
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PMID:32523942 - SUPPORT (IN_VITRO)"PHA produced from light and CO2 was 8.8 fold increased"
DNT Biotransformation with Photosynthetic Carbon Input
CROSS_FEEDINGSource Taxon: Pseudomonas putida engineered sucrose-utilizing partner
Metabolites: 2,4-dinitrotoluene (CHEBI:920), sucrose (CHEBI:17992)
Biological Processes:
- xenobiotic metabolic process (GO:0006805)
Evidence
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PMID:32064751 - SUPPORT (IN_VITRO)"degrade the environmental pollutant 2,4-dinitrotoluene (2,4-DNT)"
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PMID:32064751 - SUPPORT (IN_VITRO)"remediate the industrial pollutant 2,4-DNT while simultaneously synthesizing biopolymers"
Growth-Promoting Partner Feedback
MUTUALISMSource Taxon: Pseudomonas putida engineered sucrose-utilizing partner
Target Taxon: Synechococcus elongatus PCC 7942 cscB
Metabolites: sucrose (CHEBI:17992)
Evidence
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PMID:38609451 - SUPPORT (IN_VITRO)"up to 80% increase in the growth rate and enhanced photosynthetic capacity"
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PMID:38609451 - SUPPORT (IN_VITRO)"exchange of further molecules beyond the unidirectional feeding with sucrose"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
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MassIVE/GNPS multi-omics dataset for S. elongatus-P. putida coculture
Public MassIVE/GNPS dataset for the P. putida cscRABY and S. elongatus cscB multi-omics coculture study. |
METABOLOMICS | MASSIVE | MSV000092369 |
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ProteomeXchange/iProX proteomics dataset for S. elongatus-P. putida coculture
ProteomeXchange/iProX dataset associated with the S. elongatus cscB and P. putida cscRABY synthetic coculture multi-omics study. |
METAPROTEOME | OTHER | PXD045253 |
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Exact-system publication - PHA production from light and CO2
Primary publication establishing the S. elongatus cscB and P. putida cscAB species-pair platform for PHA production. |
OTHER | doi:10.1186/s13068-017-0875-0 |
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Exact-system publication - nitrate-blind P. putida PHA improvement
Follow-up publication using the same S. elongatus/P. putida species pair with engineered nitrate-blind P. putida for improved PHA production. |
OTHER | PMID:32523942 |
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Exact-system publication - DNT biotransformation
DOE-linked publication extending the S. elongatus/P. putida species-pair platform to 2,4-dinitrotoluene biotransformation and PHA production. |
OTHER | PMID:32064751 |
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Exact-system publication - multi-omics interaction study
Multi-omics publication on the same S. elongatus/P. putida species pair, using S. elongatus cscB and P. putida cscRABY to characterize interaction mechanisms. |
OTHER | PMID:38609451 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Light and CO2 | Illumination with CO2 as primary carbon input | N/A |
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| Nitrogen limitation and nitrate-blind engineering | Nitrogen-limited or nitrate-blind P. putida conditions | N/A |
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