A defined thermophilic anaerobic two-member syntrophic coculture in which Thermacetogenium phaeum strain PB oxidizes acetate using the reversible Wood-Ljungdahl/CO dehydrogenase-acetyl-CoA pathway, while Methanothermobacter thermautotrophicus strain TM consumes reduced products through hydrogenotrophic methanogenesis. This model system is relevant to anaerobic digestion and methane cycling because acetate oxidation is coupled to methane formation under low hydrogen partial pressure.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Thermacetogenium phaeum PB | NCBITaxon:1089553 |
PRIMARY_DEGRADER
SYNTROPHIC_PARTNER
|
N/A |
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| Methanothermobacter thermautotrophicus TM | NCBITaxon:145262 |
SYNTROPHIC_PARTNER
CROSS_FEEDER
|
N/A |
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Ecological Interactions
Syntrophic Acetate Oxidation by Thermacetogenium
SYNTROPHYSource Taxon: Thermacetogenium phaeum PB
Target Taxon: Methanothermobacter thermautotrophicus TM
Metabolites: acetate (CHEBI:30089), formate (CHEBI:15740), dihydrogen (CHEBI:18276)
Biological Processes:
- acetate catabolic process (GO:0045733)
- interspecies interaction between organisms (GO:0044419)
Evidence
-
PMID:15866934 - SUPPORT (IN_VITRO)"oxidized acetate rapidly"
-
PMID:15866934 - SUPPORT (IN_VITRO)"CO dehydrogenase/acetyl-CoA pathway reversibly"
-
PMID:31849917 - SUPPORT (IN_VITRO)"yielding formate as reduced product"
Hydrogenotrophic Methanogenesis by Methanothermobacter
SYNTROPHYSource Taxon: Methanothermobacter thermautotrophicus TM
Target Taxon: Thermacetogenium phaeum PB
Metabolites: dihydrogen (CHEBI:18276), carbon dioxide (CHEBI:16526), methane (CHEBI:16183)
Biological Processes:
- methanogenesis (GO:0015948)
Evidence
-
PMID:11872465 - SUPPORT (IN_VITRO)"thermophilic hydrogen-utilizing methanogen"
-
PMID:11872465 - SUPPORT (IN_VITRO)"hydrogen partial pressure was constantly kept very low"
-
PMID:11872465 - SUPPORT (IN_VITRO)"catalyzing the final step of methanogenesis"
Formate Transfer to the Methanogenic Partner
CROSS_FEEDINGSource Taxon: Thermacetogenium phaeum PB
Target Taxon: Methanothermobacter thermautotrophicus TM
Metabolites: formate (CHEBI:15740), carbon dioxide (CHEBI:16526)
Biological Processes:
- interspecies interaction between organisms (GO:0044419)
Evidence
-
PMID:31849917 - SUPPORT (IN_VITRO)"transferred to the methanogenic partner"
-
PMID:31849917 - SUPPORT (IN_VITRO)"quinone-dependent formate dehydrogenase"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
|
Thermacetogenium phaeum DSM 12270 genome BioProject
Genome sequencing project for T. phaeum DSM 12270 strain PB, the bacterial member of this syntrophic coculture. |
GENOME | NCBI_BIOPROJECT | PRJNA168373 |
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Exact-system pathway publication - T. phaeum and M. thermautotrophicus
Primary publication describing acetate oxidation and acetate formation by the T. phaeum and M. thermautotrophicus syntrophic coculture. |
OTHER | PMID:15866934 |
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Exact-system methanogenesis expression publication
Publication reporting methanogenesis gene expression in M. thermoautotrophicus strain TM cocultured with T. phaeum strain PB. |
OTHER | PMID:11872465 |
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Exact-system energy-conservation publication
Follow-up publication on energy-conserving enzyme systems in T. phaeum during syntrophic acetate oxidation with methanogens. |
OTHER | PMID:31849917 |
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Thermacetogenium phaeum type strain publication
Taxonomic publication for T. phaeum PB/DSM 12270 and its syntrophic acetate-oxidizing phenotype. |
OTHER | PMID:10939667 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Thermophilic temperature | 55 to 58 | degrees Celsius |
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| Anaerobic methanogenic condition | anaerobic methanogenic coculture | N/A |
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| Low hydrogen partial pressure | 20 to 80 | Pa |
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| Acetate substrate | acetate | N/A |
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