A defined hyperthermophilic bacterial-archaeal coculture in which Thermotoga maritima MSB8 ferments organic substrates and transfers hydrogen to the hydrogenotrophic methanogen historically reported as Methanococcus jannaschii, now represented by NCBI as Methanocaldococcus jannaschii DSM 2661. The coculture is relevant to DOE bioenergy and subsurface biogeochemistry because it links high-temperature fermentation, interspecies hydrogen transfer, cell aggregation, and methane formation in an experimentally tractable system.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Thermotoga maritima MSB8 | NCBITaxon:243274 |
PRIMARY_DEGRADER
SYNTROPHIC_PARTNER
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N/A |
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| Methanocaldococcus jannaschii DSM 2661 | NCBITaxon:243232 |
SYNTROPHIC_PARTNER
CROSS_FEEDER
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N/A |
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Ecological Interactions
Interspecies Hydrogen Transfer
SYNTROPHYSource Taxon: Thermotoga maritima MSB8
Target Taxon: Methanocaldococcus jannaschii DSM 2661
Metabolites: dihydrogen (CHEBI:18276), carbon dioxide (CHEBI:16526), methane (CHEBI:16183)
Biological Processes:
- interspecies interaction between organisms (GO:0044419)
- methanogenesis (GO:0015948)
Evidence
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PMID:18636642 - SUPPORT (IN_VITRO)"Cell densities of T. maritima increased 10-fold when cocultured"
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PMID:18636642 - SUPPORT (IN_VITRO)"the methanogen was able to grow in the absence of externally supplied H(2) and CO(2)"
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PMID:16391122 - SUPPORT (IN_VITRO)"Interspecies H2 transfer resulted in three- to fivefold-higher T. maritima cell densities"
Spatially Proximate Methanogenic Coculture
COLONIZATION_FACILITATIONSource Taxon: Methanocaldococcus jannaschii DSM 2661
Target Taxon: Thermotoga maritima MSB8
Metabolites: dihydrogen (CHEBI:18276)
Biological Processes:
- interspecies interaction between organisms (GO:0044419)
Evidence
-
PMID:18636642 - SUPPORT (IN_VITRO)"could not be established if the two organisms were physically separated"
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PMID:18636642 - SUPPORT (IN_VITRO)"suggesting the importance of spatial proximity"
Coculture-Associated Aggregation and Transcriptome Shift
SYNTROPHYSource Taxon: Thermotoga maritima MSB8
Target Taxon: Methanocaldococcus jannaschii DSM 2661
Biological Processes:
- interspecies interaction between organisms (GO:0044419)
Evidence
-
PMID:16391122 - SUPPORT (IN_VITRO)"292 genes differentially expressed in T. maritima"
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PMID:16391122 - SUPPORT (IN_VITRO)"concomitant formation of exopolysaccharide (EPS)-based cell aggregates"
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PMID:16391122 - SUPPORT (IN_VITRO)"syntrophic interactions can impact the transcriptome of heterotrophs"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
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Thermotoga maritima MSB8 genome BioProject
Genome project for T. maritima MSB8, the fermentative bacterial member. |
GENOME | NCBI_BIOPROJECT | PRJNA57723 |
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Methanocaldococcus jannaschii DSM 2661 genome BioProject
Genome project for M. jannaschii DSM 2661, the methanogenic archaeal member. |
GENOME | NCBI_BIOPROJECT | PRJNA102 |
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Exact-system hydrogen-transfer publication
Primary publication reporting hydrogen transfer in T. maritima and M. jannaschii hyperthermophilic coculture. |
OTHER | PMID:18636642 |
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Exact-system transcriptome and phenotype publication
Follow-up exact-system publication showing coculture impacts on T. maritima transcriptome and aggregation. |
OTHER | PMID:16391122 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Hyperthermophilic temperature | 85 | degrees C |
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| Anaerobic methanogenic condition | anaerobic | N/A |
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