A chemotactic three-member SynCom in bioorganic fertilizer for suppressing Ralstonia solanacearum in tobacco fields.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Pseudomonas sp. | NCBITaxon:306 · TaxonMech · TraitMech |
CROSS_FEEDER
|
N/A |
|
|||
| Bacillus sp. — ontology label: Bacillus <firmicutes> | NCBITaxon:1386 · TaxonMech · TraitMech |
CROSS_FEEDER
|
N/A |
|
|||
Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Strain-specific prebiotic enhancement
Added resource compounds produced strain-specific growth and cell-free broth responses. Vitamin C increased Y364 broth antagonism; selected compounds including propionic acid and esculetin increased Y832 broth antagonism. No tested compound significantly enhanced Y878 broth inhibition relative to its mock treatment. Growth screening and broth inhibition are distinct assays, and the selected compounds did not show uniform benefit across every isolate. The resource library drew on compounds commonly found in tomato root exudates; these assays do not demonstrate that all were secreted by tobacco roots in the field.
Metabolites: L-ascorbic acid (CHEBI:29073 · MediaIngredientMech · CMMMech), propionic acid (CHEBI:30768 · MediaIngredientMech · CMMMech), esculetin (CHEBI:490095 · MediaIngredientMech · CMMMech)
Selected compound additions enhanced broth antagonism in particular isolate assays. PARTIAL for growth as the mediator: growth, broth chemistry and residual additives were not selectively separated. The Y878 null enhancement result prevents generalizing this effect to all three members, and the comparison does not establish enhanced chemotaxis or root colonization.
Evidence
-
PMID:40670700 - SUPPORT (IN_VITRO)"vitamin C, propionic acid, and esculetin significantly promoted the growth and antagonistic ability of chemotactic SynCom strains"
-
PMID:40670700 - SUPPORT (IN_VITRO)"for Y878, none of the tested compounds showed a significant difference compared with the mock treatment"
Antagonism by cheA-positive SynCom isolates
The selected Y364, Y832 and Y878 isolates showed antagonism against Ralstonia solanacearum, mutual compatibility and PCR detection of cheA. The source identifies two Pseudomonas strains and one Bacillus strain; the canonical two-row taxonomy remains a broad, incomplete representation. cheA detection supports chemotaxis potential, not directly measured root migration or colonization. Strong antagonism was also observed among cheA-negative screened isolates. Compatibility and pathogen inhibition do not establish within-SynCom cross-feeding or facilitation of another member's establishment.
PARTIAL: laboratory antagonism is a candidate contribution to the field benefit of the formulated SynCom fertilizer. The field contrast combined strains, prebiotics and an organic matrix against conventional cow-manure fertilization; it did not isolate chemotaxis, colonization or broth antagonism as the mediator. The authors explicitly identify non-chemotactic SynCom controls as future work.
Evidence
-
PMID:40670700 - SUPPORT (IN_VITRO)"exhibited strong antagonism against R. solanacearum, harbored the chemotaxis gene cheA, and showed mutual compatibility"
-
PMID:40670700 - SUPPORT (IN_VITRO)"another half of isolated strains, which lacked cheA gene, still displayed strong antagonistic activity"
Tobacco Field Protection and Yield Enhancement
In the reported tobacco field trial, the SynCom-containing bioorganic fertilizer plus prebiotics was associated with a lower disease index, lower Ralstonia qPCR abundance and nearly doubled crop yield relative to conventional fertilization. This is a positive combined-treatment result, not an independently isolated effect of chemotaxis or microbial colonization facilitation. qPCR abundance is not a direct viable-cell count, and Tax4Fun predictions of indigenous-community functions do not establish active pathways or strain-resolved establishment of the inoculum.
Evidence
-
PMID:40670700 - SUPPORT (IN_VIVO)"bioorganic fertilizer containing chemotactic SynCom can protect diseased tobacco field from R. solanacearum invasion and enhance yields"
-
PMID:40670700 - SUPPORT (IN_VIVO)"the chemotactic SynCom fertilizer nearly doubled crop yield"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN How much of the tobacco field benefit specifically depends on chemotaxis or the tested broth effects?
The field comparison combines the inoculum, prebiotics and fertilizer matrix. It does not isolate chemotaxis, and the authors explicitly identify non-chemotactic controls as future work. cheA PCR and Tax4Fun inference should not stand in for measured root migration, strain persistence or active metabolic pathways. The paper also differs between Results and Discussion on resource counts and has tomato wording in some captions despite a tobacco trial; those details are not silently reconciled or used to erase the positive field outcome.
Attaches to: ecological_interactions#Strain-specific prebiotic enhancement, ecological_interactions#Antagonism by cheA-positive SynCom isolates, ecological_interactions#Tobacco Field Protection and Yield Enhancement
1 evidence item(s)
future studies should include non-chemotactic SynCom as experimental controls
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| defined synthetic community design | Two Pseudomonas strains and one Bacillus strain selected from healthy tobacco rhizosphere isolates. | N/A |
|
||
Growth Media
Cross-Mech Suite Links
Explore related entities across the CultureBotAI X-Mech knowledge bases: