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Tribromophenol Anaerobic Bioremediation SynCom

Community ID CommunityMech:000112
Ecological State ENGINEERED
Environment laboratory bioreactor — ontology label: laboratory environment
ENVO:01001405 · HabitatMech ↗

A synthetic anaerobic community with Clostridium, Dehalobacter, and Desulfatiglans functions for 2,4,6-tribromophenol mineralization.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Dehalobacter sp. — ontology label: Dehalobacter NCBITaxon:56112 · TaxonMech · TraitMech
CROSS_FEEDER
N/A
  • PMID:25461007 - SUPPORT (IN_VITRO)
    "Anaerobic mineralization of 2,4,6-tribromophenol (2,4,6-TBP) was achieved by a synthetic anaerobe community comprising a highly enriched culture of Dehalobacter sp. phylotype FTH1 acting as a reductive debrominator; Clostridium sp. strain Ma13 acting as a hydrogen supplier via glucose fermentation; and a novel 4-chlorophenol-degrading anaerobe, Desulfatiglans parachlorophenolica strain DS."
Clostridium sp. — ontology label: Clostridium NCBITaxon:1485 · TaxonMech · TraitMech
CROSS_FEEDER
N/A
  • PMID:25461007 - SUPPORT (IN_VITRO)
    "Anaerobic mineralization of 2,4,6-tribromophenol (2,4,6-TBP) was achieved by a synthetic anaerobe community comprising a highly enriched culture of Dehalobacter sp. phylotype FTH1 acting as a reductive debrominator; Clostridium sp. strain Ma13 acting as a hydrogen supplier via glucose fermentation; and a novel 4-chlorophenol-degrading anaerobe, Desulfatiglans parachlorophenolica strain DS."
Desulfatiglans parachlorophenolica NCBITaxon:1261393 · TaxonMech · TraitMech
CROSS_FEEDER
N/A
  • PMID:25461007 - SUPPORT (IN_VITRO)
    "Anaerobic mineralization of 2,4,6-tribromophenol (2,4,6-TBP) was achieved by a synthetic anaerobe community comprising a highly enriched culture of Dehalobacter sp. phylotype FTH1 acting as a reductive debrominator; Clostridium sp. strain Ma13 acting as a hydrogen supplier via glucose fermentation; and a novel 4-chlorophenol-degrading anaerobe, Desulfatiglans parachlorophenolica strain DS."

Ecological Interactions

The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.

Ecological interaction network for Tribromophenol Anaerobic Bioremediation SynCom Bipartite graph where circles represent taxa and non-circular symbols represent interactions, with distinct shapes and colours for cross-feeding. Rare or untyped interactions share the Other symbol; exact curated types remain in the interaction details.
Taxon
Cross-feeding
Other

Clostridial Hydrogen Supply for Reductive Debromination

CROSS_FEEDING

Clostridium strain Ma13 ferments glucose to supply hydrogen for reductive debromination by the highly enriched Dehalobacter FTH1 culture. The abstract supports this functional cross-feeding, not purity of the FTH1 inoculum or a closed three-isolate community.

Source Taxon: Clostridium sp.

Target Taxon: Dehalobacter sp.

Metabolites: glucose (CHEBI:17234 · MediaIngredientMech · CMMMech), hydrogen (CHEBI:18276 · MediaIngredientMech · CMMMech)

Downstream Effects:
→ Dehalobacter Reductive Debromination of Tribromophenol

Hydrogen generated from Clostridium glucose fermentation supplies the reductive Dehalobacter activity that converts 2,4,6-TBP toward phenol.

Evidence

  • PMID:25461007 - SUPPORT (IN_VITRO)
    "Clostridium sp. strain Ma13 acting as a hydrogen supplier via glucose fermentation"

Dehalobacter Reductive Debromination of Tribromophenol

CROSS_FEEDING

Combined Ma13 and enriched FTH1 activity converts 2,4,6-tribromophenol to phenol. Phenol supplies the substrate for strain DS introduced subsequently. This sequential resource delivery does not demonstrate a reciprocal benefit to FTH1 or obligate thermodynamic syntrophy.

Source Taxon: Dehalobacter sp.

Target Taxon: Desulfatiglans parachlorophenolica

Metabolites: 2,4,6-tribromophenol (CHEBI:47696 · MediaIngredientMech · CMMMech), phenol (CHEBI:15882 · MediaIngredientMech · CMMMech)

Downstream Effects:
→ Desulfatiglans Phenol Degradation

Phenol formed by combined Ma13/FTH1 debromination provides the substrate for DS after its sequential introduction; this arrow describes the reported treatment sequence.

Evidence

  • PMID:25461007 - SUPPORT (IN_VITRO)
    "2,4,6-TBP was debrominated to phenol by the combined action of Ma13 and FTH1"
  • PMID:25461007 - SUPPORT (IN_VITRO)
    "2,4,6-TBP was debrominated to phenol by the combined action of Ma13 and FTH1, then mineralized into CO2 by sequential introduction of DS"

Desulfatiglans Phenol Degradation

Desulfatiglans parachlorophenolica strain DS mineralizes the phenol intermediate after its sequential introduction. This is a recipient degradation outcome, not an observed DS-to-FTH1 benefit. The radiotracer confirmation used ring-labeled phenol, not labeled tribromophenol.

Participating Taxa: Desulfatiglans parachlorophenolica

Metabolites: phenol (CHEBI:15882 · MediaIngredientMech · CMMMech)

Downstream Effects:
→ Anaerobic Halophenol Mineralization

Sequential DS-mediated phenol degradation completes the aromatic mineralization route after debromination. Phenol radiotracing supports this terminal step, not a measured reciprocal benefit to the upstream culture.

Evidence

  • PMID:25461007 - SUPPORT (IN_VITRO)
    "2,4,6-TBP was debrominated to phenol by the combined action of Ma13 and FTH1, then mineralized into CO2 by sequential introduction of DS"

Anaerobic Halophenol Mineralization

The abstract reports anaerobic tribromophenol mineralization through glucose-fed hydrogen supply, debromination and subsequent DS-mediated phenol degradation. The three listed functional participants include an enriched FTH1 culture; they are not a proven exhaustive three-isolate membership. Sequential assembly and the phenol radiotracer assay must not be recast as simultaneous inoculation or direct tracing of labeled tribromophenol.

Participating Taxa: Dehalobacter sp., Clostridium sp., Desulfatiglans parachlorophenolica

Metabolites: 2,4,6-tribromophenol (CHEBI:47696 · MediaIngredientMech · CMMMech), phenol (CHEBI:15882 · MediaIngredientMech · CMMMech), glucose (CHEBI:17234 · MediaIngredientMech · CMMMech)

Evidence

  • PMID:25461007 - SUPPORT (IN_VITRO)
    "Anaerobic mineralization of 2,4,6-tribromophenol (2,4,6-TBP) was achieved by a synthetic anaerobe community"
  • PMID:25461007 - SUPPORT (IN_VITRO)
    "2,4,6-TBP was debrominated to phenol by the combined action of Ma13 and FTH1, then mineralized into CO2 by sequential introduction of DS"

Knowledge gaps & discussions (1)

KNOWLEDGE_GAP OPEN Which organisms and reciprocal effects are resolved beyond the abstract-supported sequential treatment?

The final-publication abstract establishes the functional sequence and phenol radiotracer confirmation. Full text was not read, so exact-system perturbations, enrichment membership and reciprocal fitness cannot be audited here. The outcome remains experimentally supported within that access boundary.

Attaches to: ecological_interactions#Clostridial Hydrogen Supply for Reductive Debromination, ecological_interactions#Dehalobacter Reductive Debromination of Tribromophenol, ecological_interactions#Desulfatiglans Phenol Degradation, ecological_interactions#Anaerobic Halophenol Mineralization

1 evidence item(s)
IN_VITRO SUPPORT PMID:25461007

2,4,6-TBP was debrominated to phenol by the combined action of Ma13 and FTH1, then mineralized into CO2 by sequential introduction of DS

Identifies phenol as the debromination intermediate channeled through the community.

Environmental Factors

Factor Value Unit
defined synthetic community design Dehalobacter phylotype FTH1, Clostridium strain Ma13, and Desulfatiglans parachlorophenolica strain DS were combined functionally. N/A
  • PMID:25461007 - SUPPORT (IN_VITRO)
    "Anaerobic mineralization of 2,4,6-tribromophenol (2,4,6-TBP) was achieved by a synthetic anaerobe community comprising a highly enriched culture of Dehalobacter sp. phylotype FTH1 acting as a reductive debrominator; Clostridium sp. strain Ma13 acting as a hydrogen supplier via glucose fermentation; and a novel 4-chlorophenol-degrading anaerobe, Desulfatiglans parachlorophenolica strain DS."

Growth Media

anaerobic mineral medium (unspecified)

Atmosphere ANAEROBIC

Preparation notes: Optimum glucose 0.5 or 2.5 mM, sulfate (SO4 2-) 1.0 or 2.5 mM, and inoculum density equivalent to 10^4 16S rRNA gene copies/mL; complete mineralization of 23 uM 2,4,6-TBP within 35 days (0.58 umol/L/d).

Evidence

PMID:25461007

"Anaerobic mineralization of 2,4,6-tribromophenol (2,4,6-TBP) was achieved by a synthetic anaerobe community"

PMID:25461007

"The optimum concentrations of glucose, SO4(2-), and inoculum densities were 0.5 or 2.5mM, 1.0 or 2.5mM, and the densities equivalent to 10(4)copiesmL(-1) of the 16S rRNA genes, respectively. This resulted in the complete mineralization of 23μM 2,4,6-TBP within 35days"

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: laboratory bioreactor ↗ TaxonMech: Dehalobacter sp. ↗ TraitMech: Dehalobacter sp. ↗ TaxonMech: Clostridium sp. ↗ TraitMech: Clostridium sp. ↗ TaxonMech: Desulfatiglans parachlorophenolica ↗ TraitMech: Desulfatiglans parachlorophenolica ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗