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Trichococcus-Syntrophomonas-Methanospirillum Butyrate Coculture

Community ID CommunityMech:000188
Ecological State ENGINEERED
Environment anaerobic wastewater bioreactor model coculture
ENVO:01001405

A defined anaerobic three-member coculture in which fermentative Trichococcus flocculiformis strain ES5 stimulates the syntrophic butyrate-oxidizing partnership between Syntrophomonas wolfei and Methanospirillum hungatei. The system extends the classic two-member fatty-acid syntrophy model by adding a fermentative bacterium that increases butyrate consumption and methane production and aggregates with the syntrophic partners. It is relevant to DOE anaerobic carbon cycling, wastewater treatment bioreactors, and engineered methanogenic consortia.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Trichococcus flocculiformis strain ES5 NCBITaxon:82803
SECONDARY_FERMENTER CROSS_FEEDER
N/A
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "a fermentative Trichococcus flocculiformis strain ES5"
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "T. flocculiformis ES5 to aggregate with the syntrophic partners"
Syntrophomonas wolfei NCBITaxon:863
PRIMARY_DEGRADER SYNTROPHIC_PARTNER
N/A
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "a coculture of Syntrophomonas wolfei"
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "stimulated butyrate oxidation"
Methanospirillum hungatei NCBITaxon:2203
SYNTROPHIC_PARTNER CROSS_FEEDER
N/A
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "Methanospirillum hungatei"
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "hydrogen/formate-scavenging methanogenic archaea"

Ecological Interactions

Ecological interaction network for Trichococcus-Syntrophomonas-Methanospirillum Butyrate Coculture Bipartite graph where circle nodes represent taxa and colored rectangles represent ecological interactions (cross-feeding, mutualism, syntrophy, competition, commensalism).
Taxon
Cross-feeding
Mutualism
Syntrophy
Competition
Commensalism
Niche partitioning
Colonization facilitation
Strain competition
Predation

Fermenter-Stimulated Butyrate Oxidation and Methane Production

SYNTROPHY

Source Taxon: Trichococcus flocculiformis strain ES5

Target Taxon: Syntrophomonas wolfei

Metabolites: butyrate (CHEBI:17968), methane (CHEBI:16183)

Biological Processes:

Evidence

  • PMID:35699440 - SUPPORT (IN_VITRO)
    "The addition of T. flocculiformis ES5 to the syntrophic cocultures led to an increase"
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "rates of butyrate consumption (120%) and volumetric methane production (150%)"

Aggregation With Syntrophic Partners

COLONIZATION_FACILITATION

Source Taxon: Trichococcus flocculiformis strain ES5

Target Taxon: Methanospirillum hungatei

Biological Processes:

  • interspecies interaction between organisms (GO:0044419)

Evidence

  • PMID:35699440 - SUPPORT (IN_VITRO)
    "S. wolfei, M. hungatei, and T. flocculiformis ES5"
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "T. flocculiformis ES5 to aggregate with the syntrophic partners"

Proteome Response in the Syntrophic Partners

SYNTROPHY

Source Taxon: Trichococcus flocculiformis strain ES5

Target Taxon: Syntrophomonas wolfei

Biological Processes:

Evidence

  • PMID:35699440 - SUPPORT (IN_VITRO)
    "signal transducing systems of M. hungatei"
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "activation of additional butyryl coenzyme A dehydrogenase and an electron transfer flavoprotein"

Associated Datasets

Dataset Type Repository Accession
Trichococcus flocculiformis DSM 23957 genome assembly
Genome assembly for T. flocculiformis ES5/DSM 23957, the fermentative strain added to the coculture. 		GENOME OTHER GCA_900129415
Trichococcus flocculiformis DSM 23957 IMG genome
JGI IMG genome resource for T. flocculiformis DSM 23957. 		GENOME JGI_IMG 2619618835

External Resources

Name Repository Resource ID
Exact-system primary publication - ES5-stimulated S. wolfei and M. hungatei coculture
Primary publication reporting the T. flocculiformis ES5, S. wolfei, and M. hungatei three-member coculture; no additional exact-composition publication was identified during this curation pass. 		OTHER PMID:35699440
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "S. wolfei, M. hungatei, and T. flocculiformis ES5"
DOI landing page - ES5-stimulated syntrophic coculture
DOI landing page for the exact-system primary publication. 		OTHER doi:10.1128/aem.00391-22
NCBI Taxonomy - Trichococcus flocculiformis
NCBI Taxonomy record for Trichococcus flocculiformis. 		OTHER NCBITaxon:82803
BacDive strain record - Trichococcus flocculiformis ES5
BacDive record for T. flocculiformis ES5/DSM 23957, including NCBI taxon, culture collection, isolation, and genome links. 		OTHER BacDive:2286
NCBI Taxonomy - Syntrophomonas wolfei
NCBI Taxonomy record for Syntrophomonas wolfei. 		OTHER NCBITaxon:863
NCBI Taxonomy - Methanospirillum hungatei
NCBI Taxonomy record for Methanospirillum hungatei. 		OTHER NCBITaxon:2203

Environmental Factors

Factor Value Unit
Anoxic anaerobic ecosystem context anoxic sediments, soils, and wastewater treatment bioreactors N/A
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "anoxic sediments, soils, and wastewater treatment bioreactors"
Fermentative bacterium addition Trichococcus flocculiformis strain ES5 added N/A
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "due to the addition of a fermentative Trichococcus flocculiformis strain ES5"
Butyrate and methane rate response increased butyrate consumption and methane production N/A
  • PMID:35699440 - SUPPORT (IN_VITRO)
    "rates of butyrate consumption (120%) and volumetric methane production (150%)"

Growth Media

Anaerobic butyrate syntrophic coculture assay

Atmosphere ANAEROBIC

Composition

Component Concentration Unit CHEBI
Butyrate N/A N/A CHEBI:17968

Preparation notes: Abstract-level evidence supports an anaerobic syntrophic coculture assayed for butyrate oxidation and methane generation; detailed medium composition is not asserted here.

Evidence

PMID:35699440

"stimulated butyrate oxidation and methane generation"

PMID:35699440

"syntrophic anaerobic consortia"