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Trichoderma lentiforme and Burkholderia Cross-Feeding Atrazine-Degrading Consortium

Community ID CommunityMech:000479
Ecological State ENGINEERED
Environment contaminated soil
ENVO:00002116 · HabitatMech ↗

Cross-kingdom fungal-bacterial synthetic consortium pairing the atrazine-degrading fungus Trichoderma lentiforme HN154 with rhizosphere Burkholderia isolates (strains Bur-4, Bur-5, and Bur-14) to remediate atrazine-contaminated corn-soybean rotation soil and protect soybean (Glycine max) from residual phytotoxicity. Trichoderma lentiforme HN154 transforms atrazine into hydroxyatrazine and biuret intermediates that stimulate Burkholderia chemotaxis, swarming, and swimming motility; cross-feeding on these intermediates harnesses bacterial atzB, atzC, atzE, and atzF catabolic enzymes to achieve 86.3% atrazine degradation within 168 h while boosting soybean SOD, POD, and CAT antioxidant defenses.

Taxonomy

Taxon Ontology ID Functional Roles Abundance
Trichoderma lentiforme NCBITaxon:1567552 · TaxonMech · TraitMech
PRIMARY_DEGRADER CROSS_FEEDER
DOMINANT
  • PMID:42372060 - SUPPORT (IN_VIVO)
    "Mechanistically, intermediates (hydroxyatrazine and biuret) generated during T. lentiforme HN154-mediated degradation stimulated Burkholderia chemotaxis, swarming and swimming motility, while cross-feeding on these metabolites synergistically accelerated bioaugmentation (the Trichoderma-Burkholderia synthetic consortium achieved rapid atrazine degradation of 86.3% within 168 h)."
Burkholderia NCBITaxon:32008 · TaxonMech · TraitMech
SECONDARY_FERMENTER SYNTROPHIC_PARTNER
DOMINANT
  • PMID:42372060 - SUPPORT (IN_VITRO)
    "Among 23 rhizosphere isolates, the Burkholderia strains Bur-4, Bur-5, and Bur-14 showed the highest atrazine degradation rates (26.3% - 29.4%) within 72 h."

Ecological Interactions

The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.

Ecological interaction network for Trichoderma lentiforme and Burkholderia Cross-Feeding Atrazine-Degrading Consortium Bipartite graph where circles represent taxa and non-circular symbols represent interactions, with distinct shapes and colours for cross-feeding, mutualism.
Taxon
Cross-feeding
Mutualism

Fungal Atrazine Transformation and Chemotactic Recruitment of Burkholderia

CROSS_FEEDING

Trichoderma lentiforme HN154 initiates atrazine transformation into hydroxyatrazine and biuret intermediates, which stimulate Burkholderia chemotaxis, swarming, and swimming motility and enrich Burkholderiaceae encoding atzB, atzC, atzE, and atzF.

Source Taxon: Trichoderma lentiforme

Target Taxon: Burkholderia

Metabolites: atrazine (CHEBI:15930 · MediaIngredientMech · CMMMech)

Biological Processes:

Downstream Effects:
→ Fungal-to-Bacterial Cross-Feeding of Hydroxyatrazine and Biuret Intermediates

DIRECTLY IMPLIED: Chemotactic recruitment and motility stimulation of Burkholderia by Trichoderma-derived hydroxyatrazine and biuret enable cross-feeding and synergistic atrazine mineralization.

Evidence

  • PMID:42372060 - SUPPORT (IN_VIVO)
    "Metagenomic analysis revealed that colonization by T. lentiforme HN154 drove restructuring of microbial networks, enriching the keystone family Burkholderiaceae, which was strongly associated with atrazine catabolism and four key catabolic enzymes (EC 3.5.4.43 (atzB), EC 3.5.1.131 (atzE), EC 3.5.1.54 (atzF), EC 3.5.4.42 (atzC))."
  • PMID:42372060 - SUPPORT (IN_VITRO)
    "Mechanistically, intermediates (hydroxyatrazine and biuret) generated during T. lentiforme HN154-mediated degradation stimulated Burkholderia chemotaxis, swarming and swimming motility, while cross-feeding on these metabolites synergistically accelerated bioaugmentation (the Trichoderma-Burkholderia synthetic consortium achieved rapid atrazine degradation of 86.3% within 168 h)."

Fungal-to-Bacterial Cross-Feeding of Hydroxyatrazine and Biuret Intermediates

CROSS_FEEDING

Trichoderma lentiforme HN154 transforms atrazine into hydroxyatrazine and biuret intermediates that stimulate Burkholderia chemotaxis, swarming, and swimming motility; Burkholderia strains (encoding atzB, atzC, atzE, and atzF) cross-feed on these intermediates to achieve 86.3% atrazine removal within 168 h.

Source Taxon: Trichoderma lentiforme

Target Taxon: Burkholderia

Metabolites: atrazine (CHEBI:15930 · MediaIngredientMech · CMMMech)

Biological Processes:

Downstream Effects:
→ Alleviation of Atrazine Phytotoxicity and Enhancement of Soybean Antioxidant Defense

DIRECTLY IMPLIED: Synergistic cross-feeding and rapid atrazine removal by the Trichoderma-Burkholderia consortium alleviate herbicide phytotoxicity and boost antioxidant enzyme defenses in soybean.

Evidence

  • PMID:42372060 - SUPPORT (IN_VITRO)
    "Mechanistically, intermediates (hydroxyatrazine and biuret) generated during T. lentiforme HN154-mediated degradation stimulated Burkholderia chemotaxis, swarming and swimming motility, while cross-feeding on these metabolites synergistically accelerated bioaugmentation (the Trichoderma-Burkholderia synthetic consortium achieved rapid atrazine degradation of 86.3% within 168 h)."
  • PMID:42372060 - SUPPORT (IN_VIVO)
    "A Trichoderma-Burkholderia synthetic consortium further enhanced remediation by boosting plant antioxidant defenses (SOD, POD, CAT) and reducing oxidative damage (MDA)."

Alleviation of Atrazine Phytotoxicity and Enhancement of Soybean Antioxidant Defense

MUTUALISM

Bioaugmentation with the Trichoderma lentiforme HN154 and Burkholderia synthetic consortium removes 86.3% of atrazine within 168 h and alleviates soybean phytotoxicity by increasing SOD, POD, and CAT antioxidant activities and lowering malondialdehyde (MDA) oxidative damage.

Source Taxon: Burkholderia

Target Taxon: Trichoderma lentiforme

Metabolites: atrazine (CHEBI:15930 · MediaIngredientMech · CMMMech)

Biological Processes:

Evidence

  • PMID:42372060 - SUPPORT (IN_VIVO)
    "A Trichoderma-Burkholderia synthetic consortium further enhanced remediation by boosting plant antioxidant defenses (SOD, POD, CAT) and reducing oxidative damage (MDA)."

Environmental Factors

Factor Value Unit
Soil Atrazine Contamination 500 mg/kg atrazine in corn-soybean rotation soil N/A
  • PMID:42372060 - SUPPORT (IN_VIVO)
    "By leveraging the atrazine-degrading fungus Trichoderma lentiforme HN154, we achieved an 80.3% removal of atrazine (500 mg/kg) in non-sterilized soils from a corn planting system within 14 days, 22.1% higher degradation than in sterilized soil, while concurrently alleviating phytotoxic symptoms in soybean plants."

Cross-Mech Suite Links

Explore related entities across the CultureBotAI X-Mech knowledge bases:

HabitatMech: contaminated soil ↗ TaxonMech: Trichoderma lentiforme ↗ TraitMech: Trichoderma lentiforme ↗ TaxonMech: Burkholderia ↗ TraitMech: Burkholderia ↗ CultureMech Growth Media ↗ PathwayMech Pathways ↗