Functional anaerobic organochlorine-degrading bacterial consortia (top-down consortium M and bottom-up consortium Y13) constructed around generalist non-obligate organochlorine-degrading Enterococcus strains (Enterococcus casseliflavus PseS3, R1, R3, R12) alongside organohalide-respiring and fermentative partners (Sulfurospirillum multivorans, Trichlorobacter lovleyi, Dehalococcoides mccartyi, and Petrimonas sulfuriphila) for gamma-hexachlorocyclohexane (lindane) bioremediation in coastal wetland sediment microcosms while suppressing CO2-reduction methanogenesis.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Enterococcus casseliflavus | NCBITaxon:37734 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
SYNTROPHIC_PARTNER
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DOMINANT |
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| Sulfurospirillum multivorans | NCBITaxon:66821 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
SYNTROPHIC_PARTNER
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COMMON |
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| Trichlorobacter lovleyi | NCBITaxon:313985 · TaxonMech · TraitMech |
SYNTROPHIC_PARTNER
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COMMON |
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| Petrimonas sulfuriphila | NCBITaxon:285070 · TaxonMech · TraitMech |
SECONDARY_FERMENTER
SYNTROPHIC_PARTNER
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COMMON |
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| Dehalococcoides mccartyi | NCBITaxon:61435 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
SYNTROPHIC_PARTNER
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COMMON |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Enterococcus Colonization and pceA Reductive Dehalogenase Gene Enrichment
SYNTROPHYColonization of inoculated sediment microcosms by adaptable non-obligate organochlorine-degrading bacteria including Enterococcus casseliflavus together with obligate organohalide respirers enriches the reductive dehalogenase gene pceA on mobile genetic elements.
Participating Taxa: Enterococcus casseliflavus, Sulfurospirillum multivorans, Trichlorobacter lovleyi, Petrimonas sulfuriphila, Dehalococcoides mccartyi
Biological Processes:
- organohalide respiration (GO:0070812 · PathwayMech)
- halogenated hydrocarbon metabolic process (GO:0042197 · PathwayMech)
DIRECTLY IMPLIED: Colonization by organochlorine-degrading bacteria and enrichment of pceA dehalogenase genes drive efficient anaerobic γ-HCH degradation in wetland sediment microcosms.
Evidence
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PMID:42663479 - SUPPORT (IN_VITRO)"The well-known degradation gene pceA was enriched in the Y13 group, and the co-localization of pceA-encoding contigs with mobile genetic elements was identified."
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PMID:42663479 - SUPPORT (IN_VITRO)"Notably, Enterococcus was found to have successfully colonized inoculated groups. It has strong environmental evolutionary adaptability and might acquire organochlorine-degrading genes through horizontal gene transfer under pollution stress."
Cooperative Organochlorine Dechlorination of Gamma-Hexachlorocyclohexane
SYNTROPHYConstructed functional consortia combining generalist non-obligate organochlorine-degrading bacteria (including Enterococcus casseliflavus, Sulfurospirillum multivorans, Trichlorobacter lovleyi, and Petrimonas sulfuriphila) with Dehalococcoides mccartyi and enriched pceA reductive dehalogenase genes cooperatively degrade gamma-hexachlorocyclohexane (lindane) in anoxic wetland sediment microcosms.
Participating Taxa: Enterococcus casseliflavus, Sulfurospirillum multivorans, Trichlorobacter lovleyi, Petrimonas sulfuriphila, Dehalococcoides mccartyi
Biological Processes:
- organohalide respiration (GO:0070812 · PathwayMech)
- halogenated hydrocarbon metabolic process (GO:0042197 · PathwayMech)
DIRECTLY IMPLIED: Bioaugmentation with the organochlorine-degrading consortia redirects electron flow toward reductive dechlorination and suppresses CO2-reduction methanogenesis.
Evidence
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PMID:42663479 - SUPPORT (IN_VITRO)"Both consortia demonstrated efficient degradation in sediment microcosms, reducing γ-HCH (C/C₀) to 63.0% (M) and 56.1% (Y13)."
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PMID:42663479 - SUPPORT (IN_VITRO)"Simultaneously, functional microbial consortia inoculation significantly suppressed the CO₂ reduction methanogenesis pathway compared with the non-inoculated group (P < 0.05), as evidenced by a marked decline in the CH₄/CO₂ ratio in inoculated groups."
Suppression of Carbon Dioxide Reduction Methanogenesis During Lindane Bioremediation
SYNTROPHYInoculation of the organochlorine-degrading consortia into wetland sediment microcosms significantly suppresses the CO2-reduction methanogenesis pathway and lowers the CH4/CO2 emission ratio.
Participating Taxa: Enterococcus casseliflavus, Sulfurospirillum multivorans, Trichlorobacter lovleyi, Petrimonas sulfuriphila, Dehalococcoides mccartyi
Metabolites: methane (CHEBI:16183 · MediaIngredientMech · CMMMech), carbon dioxide (CHEBI:16526 · MediaIngredientMech · CMMMech)
Biological Processes:
- methanogenesis, from carbon dioxide (GO:0019386 · PathwayMech)
Evidence
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PMID:42663479 - SUPPORT (IN_VITRO)"Simultaneously, functional microbial consortia inoculation significantly suppressed the CO₂ reduction methanogenesis pathway compared with the non-inoculated group (P < 0.05), as evidenced by a marked decline in the CH₄/CO₂ ratio in inoculated groups."
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