Freshwater wetland soil microbial microcosms incubated under anoxic non-sulfate, anoxic sulfate-addition, oxic non-sulfate, and oxic sulfate-addition conditions to resolve how climate-linked oxygen and sulfate exposures alter methane and carbon dioxide emissions. The study integrated geochemical measurements, proteogenomics, metaproteomics, and stoichiometric modeling to identify altered microbial guilds and metabolic processes driving CH4 and CO2 emissions. Sulfate exposure reduced methane production, oxygen shifted greenhouse gas emissions from CH4 toward CO2, and the combined exposure resembled oxygen exposure alone.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| wetland soil microbial community | NCBITaxon:131567 |
PRIMARY_DEGRADER
SECONDARY_FERMENTER
SYNTROPHIC_PARTNER
|
N/A |
|
|||
| wetland soil methanogenic archaea | NCBITaxon:2157 |
SYNTROPHIC_PARTNER
PRIMARY_PRODUCER
|
N/A |
|
|||
| wetland soil bacterial guilds | NCBITaxon:2 |
PRIMARY_DEGRADER
SECONDARY_FERMENTER
CROSS_FEEDER
|
N/A |
|
|||
Ecological Interactions
Sulfate Suppression Of Methanogenesis
COMPETITIONSource Taxon: wetland soil bacterial guilds
Target Taxon: wetland soil methanogenic archaea
Metabolites: sulfate (CHEBI:16189), methane (CHEBI:16183)
Biological Processes:
- sulfate reduction (GO:0019419)
- methanogenesis (GO:0015948)
Evidence
-
PMID:38961111 - SUPPORT (IN_VITRO)"Elevated SO42- reduced CH4 emissions"
-
PMID:38961111 - SUPPORT (IN_VITRO)"hydrogenotrophic methanogenesis more suppressed than acetoclastic"
Oxygen Shift From Methane To Carbon Dioxide
NICHE_PARTITIONINGMetabolites: dioxygen (CHEBI:15379), methane (CHEBI:16183), carbon dioxide (CHEBI:16526)
Biological Processes:
- methanogenesis (GO:0015948)
Evidence
-
PMID:38961111 - SUPPORT (IN_VITRO)"Elevated O2 shifted the greenhouse gas emissions from CH4 to CO2"
-
PMID:38961111 - SUPPORT (IN_VITRO)"combined SO42- and O2 exposures on CH4 and CO2 emissions were similar to those of O2 exposure alone"
Lactate-Sulfate Carbon And Sulfur Subnetwork
CROSS_FEEDINGSource Taxon: wetland soil bacterial guilds
Metabolites: lactate (CHEBI:24996), sulfate (CHEBI:16189), acetate (CHEBI:30089), hydrogen sulfide (CHEBI:16136), carbon dioxide (CHEBI:16526)
Biological Processes:
- sulfate reduction (GO:0019419)
- organic substance catabolic process (GO:0071704)
Evidence
-
PMID:38961111 - SUPPORT (COMPUTATIONAL)"Metaproteomics and stoichiometric modeling revealed a unique subnetwork involving carbon metabolism"
-
PMID:38961111 - SUPPORT (COMPUTATIONAL)"converts lactate and SO42- to produce acetate, H2S, and CO2"
Associated Datasets
| Dataset | Type | Repository | Accession |
|---|---|---|---|
|
Wetland oxygen-sulfate metagenome BioProject
NCBI BioProject for metagenome data from the wetland soil incubation treated with sulfate and oxygen. |
METAGENOME | NCBI_BIOPROJECT | PRJNA1112840 |
|
Wetland oxygen-sulfate metaproteome dataset
ProteomeXchange/PRIDE dataset linked to the wetland soil incubation treated with sulfate and oxygen. |
METAPROTEOME | OTHER | PXD047453 |
External Resources
| Name | Repository | Resource ID |
|---|---|---|
|
Primary publication for wetland oxygen-sulfate greenhouse gas microcosms
PubMed record for the npj Biofilms and Microbiomes article reporting metaproteomics-informed stoichiometric modeling of wetland microbial community responses to oxygen and sulfate exposure. |
OTHER | PMID:38961111 |
|
||
|
DOI landing page
DOI landing page for the npj Biofilms and Microbiomes publication. |
OTHER | doi:10.1038/s41522-024-00525-5 |
|
||
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Oxygen exposure | oxic microcosm treatment | N/A |
|
||
| Sulfate exposure | elevated sulfate microcosm treatment | N/A |
|
||
| Greenhouse gas emissions | methane and carbon dioxide | N/A |
|
||