A four-member wheat-rhizosphere synthetic consortium (SynCom 1/C1) composed of Pseudomonas sp. B5, Pseudomonas sp. B11, Pseudomonas sp. B12, and Pseudomonas sp. P25. Yin et al. reported that C1 inhibited Rhizoctonia solani AG8 radial growth in dual culture and significantly reduced wheat root rot while increasing wheat fresh root weight under AG8 challenge.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Pseudomonas sp. B5 — ontology label: Pseudomonas | NCBITaxon:286 · TaxonMech · TraitMech |
PATHOGEN_ANTAGONIST
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DOMINANT |
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| Pseudomonas sp. B11 — ontology label: Pseudomonas | NCBITaxon:286 · TaxonMech · TraitMech |
PATHOGEN_ANTAGONIST
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DOMINANT |
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| Pseudomonas sp. B12 — ontology label: Pseudomonas | NCBITaxon:286 · TaxonMech · TraitMech |
PATHOGEN_ANTAGONIST
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DOMINANT |
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| Pseudomonas sp. P25 — ontology label: Pseudomonas | NCBITaxon:286 · TaxonMech · TraitMech |
PATHOGEN_ANTAGONIST
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DOMINANT |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
C1 SynCom inhibition of R. solani AG8 radial growth
The assembled C1 inhibited Rhizoctonia solani AG8 radial growth in dual culture, with reported inhibition of 37.00% (SD 6.57) in Table 2. This is a positive fungal-growth outcome, not evidence that both bacterial and fungal fitness declined. Plate inhibition does not establish the mediator or guarantee host protection.
Participating Taxa: Pseudomonas sp. B5, Pseudomonas sp. B11, Pseudomonas sp. B12, Pseudomonas sp. P25
Biological Processes:
- interspecies interaction (GO:0044419 · PathwayMech)
Evidence
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PMID:36118206 - SUPPORT (IN_VITRO)"In vitro dual-culture assay showed eight SynComs, including C1-C4, C6-C8, and C10, inhibited the growth of R. solani AG8 to varying degrees"
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PMID:36118206 - SUPPORT (IN_VITRO)"SynCom 1(C1)37.00 ± 6.57"
C1 SynCom protection of wheat under AG8 challenge
C1 reduced root-rot scores and increased fresh root weight of AG8-challenged Alpowa wheat in the three-week greenhouse soil assay. Preserve this positive whole-mixture benefit. The study did not show superiority over the best individual strain or establish reciprocal microbial benefit, bilateral competition, inoculum persistence, or a unique IAA/volatile mechanism. Only B5 among the four C1 members significantly reduced disease when tested individually.
Participating Taxa: Pseudomonas sp. B5, Pseudomonas sp. B11, Pseudomonas sp. B12, Pseudomonas sp. P25
Biological Processes:
- biological process involved in interspecies interaction between organisms (GO:0044419 · PathwayMech)
Evidence
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PMID:36118206 - SUPPORT (IN_VIVO)"SynCom C1, composed of four different Pseudomonas members, significantly reduced wheat root rot and increased root weight, compared with the controls"
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PMID:36118206 - SUPPORT (IN_VIVO)"None of them was significantly better than a single strain"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN Which processes explain C1 protection beyond its separate laboratory phenotypes?
C1 is protective in soil, yet individual-versus-mixture phenotypes differ. The paper reports no antifungal activity from any SynCom cell-free supernatant and identifies C2-C4/C7, not C1, as significant volatile mixtures. Lower C1 auxin and volatile outputs are not selective proof that these routes are impossible. Inoculum persistence was explicitly not assessed. Equal-ratio preparation remains in metadata and the earlier removal of workflow arrows remains intact.
Attaches to: ecological_interactions#C1 SynCom inhibition of R. solani AG8 radial growth, ecological_interactions#C1 SynCom protection of wheat under AG8 challenge
3 evidence item(s)
None of them was significantly better than a single strain
the cell-free supernatants from the rest of the eight bacteria and 10 SynComs did not show any antifungal activities
While not assessed here, investigation of the fate of those inoculations in the soil after plant growth is needed to address their persistence and stability
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Host plant | Triticum aestivum | N/A |
| Target pathogen | Rhizoctonia solani AG8 | N/A |
| Application | biological control | N/A |
Growth Media
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