A four-member bacterial-fungal synthetic microbial community that produces enzymes for wheat straw pretreatment and enhanced biogas production.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Bacillus subtilis | NCBITaxon:1423 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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N/A |
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| Acinetobacter johnsonii | NCBITaxon:40214 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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N/A |
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| Trichoderma viride | NCBITaxon:5547 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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N/A |
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| Aspergillus niger | NCBITaxon:5061 · TaxonMech · TraitMech |
PRIMARY_DEGRADER
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N/A |
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Ecological Interactions
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
Synthetic Community Fermentation-Enzyme Production
The four reported bacterial/fungal degraders form an enzyme-producing inoculum that yields the crude enzyme solution used for wheat-straw pretreatment. This positive process output does not measure reciprocal fitness, member-specific enzyme contributions or metabolite cross-feeding. The accessible abstract does not resolve strain identities or establish that the four isolates constitute the downstream anaerobic-digestion community.
Metabolites: lignocellulose (CHEBI:180683 · MediaIngredientMech · CMMMech)
Biological Processes:
- cellulose catabolic process (GO:0030245 · PathwayMech)
- xylan catabolic process (GO:0045493 · PathwayMech)
- lignin catabolic process (GO:0046274 · PathwayMech)
Fermentation supplies the crude enzyme solution applied to wheat straw, enabling the measured polymer-degradation pretreatment. This is an enzyme-mediated material-transfer step, not proof that the living four-member inoculum colonizes every subsequent process stage.
Evidence
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PMID:38748977 - SUPPORT (IN_VITRO)"synthetic microbial communities were constructed for fermentation-enzyme production"
Lignocellulose Enzyme Pretreatment
Crude enzyme solution from the synthetic community was applied to wheat straw before anaerobic digestion. Reported cellulose, hemicellulose and lignin degradation rates were 39.85%, 36.99% and 19.21%, respectively. The treatment acts through the enzyme preparation; these values do not establish reciprocal microbial benefit or living producer growth on the pretreated substrate.
Metabolites: cellulose (CHEBI:18246 · MediaIngredientMech · CMMMech), hemicellulose (CHEBI:61266 · MediaIngredientMech · CMMMech), lignin (CHEBI:6457 · MediaIngredientMech · CMMMech)
Biological Processes:
- cellulose catabolic process (GO:0030245 · PathwayMech)
- xylan catabolic process (GO:0045493 · PathwayMech)
- lignin catabolic process (GO:0046274 · PathwayMech)
The enzyme-pretreated straw supplies substrate to subsequent anaerobic digestion, where cumulative biogas was higher than with untreated straw or either commercial-enzyme pretreatment. Retain this process-level treatment effect without assigning gas production to the four enzyme producers or to one uniquely necessary polymer-degradation route.
Evidence
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PMID:38748977 - SUPPORT (IN_VITRO)"Herein, a metagenomic approach was used to screen core microorganisms (Bacillus subtilis, Acinetobacter johnsonii, Trichoderma viride, and Aspergillus niger) possessing lignocellulose-degrading abilities among samples from three environments: pile retting wheat straw (WS), WS returned to soil, and forest soil."
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PMID:38748977 - SUPPORT (IN_VITRO)"yielding cellulose, hemicellulose, and lignin degradation rates of 39.85, 36.99, and 19.21%, respectively."
Enhanced Biogas Production after Anaerobic Digestion
Subsequent anaerobic digestion of synthetic-community enzyme-pretreated straw yielded 801.16 mL/g TS cumulative biogas, higher than untreated straw and both commercial-enzyme pretreatments. Biogas volume is not a methane-only yield. The abstract does not identify the digestion community or demonstrate methanogenesis by the four enzyme-producing isolates.
Evidence
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PMID:38748977 - SUPPORT (IN_VITRO)"SMCEP displayed the highest cumulative biogas production at 801.16 mL/g TS"
Knowledge gaps & discussions (1)
KNOWLEDGE_GAP OPEN How do enzyme producers and the downstream digestion community contribute to the biogas benefit?
The accessible abstract supports the existing enzyme-production to pretreatment to digestion sequence and positive polymer and total-biogas outcomes. It does not resolve individual-strain contributions, reciprocal benefit, digestor membership, methane fraction or a uniquely necessary enzyme. Primary full methods and supplementary information were not inspected; publisher access attempts failed. This is a bounded access limitation, not a claim that full text or supplements do not exist.
Attaches to: ecological_interactions#Synthetic Community Fermentation-Enzyme Production, ecological_interactions#Lignocellulose Enzyme Pretreatment, ecological_interactions#Enhanced Biogas Production after Anaerobic Digestion
1 evidence item(s)
SMCEP displayed the highest cumulative biogas production at 801.16 mL/g TS
Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| defined synthetic community design | Core microorganisms with lignocellulose-degrading abilities were screened from straw and forest environments and combined. | N/A |
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