A defined synthetic obligate mutualism pairing auxotrophic Zymomonas mobilis with auxotrophic Escherichia coli. Exometabolomics of Z. mobilis spent medium identified amino acid exometabolites used to select E. coli proA, pheA, and ilvA auxotroph partners, while mutant fitness profiling selected a Z. mobilis ZMO0748 auxotroph whose rescue was linked to glutathione-related metabolites in E. coli spent medium. The system is DOE-relevant because it was developed by LBNL and Sandia researchers with DOE BER support as a synthetic microbial ecology platform for more resilient biotechnology processes.
Taxonomy
| Taxon | Ontology ID | Functional Roles | Abundance |
|---|---|---|---|
| Zymomonas mobilis | NCBITaxon:542 |
CROSS_FEEDER
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N/A |
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| Escherichia coli | NCBITaxon:562 |
CROSS_FEEDER
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N/A |
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Ecological Interactions
Zymomonas Amino Acid Rescue of E. coli Auxotrophs
CROSS_FEEDINGSource Taxon: Zymomonas mobilis
Target Taxon: Escherichia coli
Metabolites: amino acid (CHEBI:33709)
Biological Processes:
- interspecies interaction between organisms (GO:0044419)
Evidence
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PMID:26885935 - SUPPORT (IN_VITRO)"Amino acid exometabolites identified in the spent medium of Z. mobilis"
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PMID:26885935 - SUPPORT (IN_VITRO)"used to select three corresponding E. coli auxotrophs"
E. coli Glutathione-Related Rescue of Zymomonas Auxotroph
CROSS_FEEDINGSource Taxon: Escherichia coli
Target Taxon: Zymomonas mobilis
Metabolites: glutathione (CHEBI:16856)
Biological Processes:
- interspecies interaction between organisms (GO:0044419)
Evidence
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PMID:26885935 - SUPPORT (IN_VITRO)"identified glutathione related metabolites as potentially available for rescue"
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PMID:26885935 - SUPPORT (IN_VITRO)"rescue of the Z. mobilis cysteine synthase mutant"
Reciprocal Auxotroph Coculture Growth
MUTUALISMSource Taxon: Zymomonas mobilis
Target Taxon: Escherichia coli
Biological Processes:
- interspecies interaction between organisms (GO:0044419)
Evidence
-
PMID:26885935 - SUPPORT (IN_VITRO)"Three sets of cocultures between the Z. mobilis auxotroph and each of the three E. coli auxotrophs"
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PMID:26885935 - SUPPORT (IN_VITRO)"monitored by optical density for growth"
External Resources
| Name | Repository | Resource ID |
|---|---|---|
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Primary publication - exometabolomics-designed obligate mutualism
Primary publication reporting exometabolomics-assisted design and validation of Z. mobilis and E. coli synthetic obligate mutualisms. |
OTHER | PMID:26885935 |
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DOI landing page - exometabolomics-designed obligate mutualism
DOI landing page for the ACS Synthetic Biology article. |
OTHER | doi:10.1021/acssynbio.5b00236 |
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DOE PAGES record for the exact publication
DOE PAGES metadata for the publication, including LBNL and Sandia affiliations and DOE BER sponsorship. |
OTHER | OSTI:1429352 |
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Environmental Factors
| Factor | Value | Unit |
|---|---|---|
| Auxotrophic mutant selection | Z. mobilis ZMO0748 auxotroph with E. coli proA, pheA, or ilvA auxotrophs | N/A |
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| Exometabolomics-guided partner selection | Mass spectrometry-based exometabolomics of spent media | N/A |
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