hCom2 Complex Gut Microbiome

Community ID CommunityMech:000123

Ecological State ENGINEERED

Environment human gut
UBERON:0001007

A complex defined human gut microbiome assembled from hCom1 and iteratively augmented after fecal challenge to improve stability and colonization resistance in gnotobiotic mice.

Taxonomy

Taxon	Ontology ID	Functional Roles	Abundance
defined human gut bacterial members	NCBITaxon:2	CROSS_FEEDER	N/A
PMID:36070752 - SUPPORT (IN_VIVO) "We identified new species that engrafted following fecal challenge and added them to hCom1, yielding hCom2."

Ecological Interactions

Taxon

Colonization facilitation

Gut Niche Filling and Colonization Resistance

COLONIZATION_FACILITATION

Evidence

PMID:36070752 - SUPPORT (IN_VIVO)

"We identified new species that engrafted following fecal challenge and added them to hCom1, yielding hCom2."

Environmental Factors

Factor	Value	Unit
gnotobiotic fecal challenge	Germ-free mice colonized with hCom1 were challenged with a human fecal sample to identify additional engrafting species.	N/A
PMID:36070752 - SUPPORT (IN_VIVO) "We identified new species that engrafted following fecal challenge and added them to hCom1, yielding hCom2."

Growth Media

SAAC complete defined medium

Atmosphere ANAEROBIC

Preparation notes: Individual strains were propagated in Mega Medium (MM) or Chopped Meat Medium (CMM) and diluted 1:10 every 24 h before assembly; the assembled community was propagated in vitro in SAAC complete defined medium.

Evidence

PMID:36070752

"The community was propagated in vitro in SAAC medium"

PMID:36070752

"All culturing was done in an anaerobic chamber (Coy Laboratories) at 10% CO2, 5% H2, and 85% N2 atmosphere."

PMID:36070752

"each of our 104 strains can be propagated in Mega Medium (MM), Chopped Meat Medium (CMM), or both"

PMID:36070752

"Strains were diluted 1:10 every 24 h for 2 days into fresh growth medium in 2.2-mL deep well plates"