Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_MHY.pdf
Composition
flowchart LR
CultureMech_000091["mhy"]
ing0_KNO["KNO"]
CultureMech_000091 --> ing0_KNO
CHEBI_63043["CHEBI:63043"]
ing0_KNO -.-> CHEBI_63043
ing1_Ca_NO3_2_4H2O["Ca(NO3)2.4H2O"]
CultureMech_000091 --> ing1_Ca_NO3_2_4H2O
ing2_KH_PO["KH PO"]
CultureMech_000091 --> ing2_KH_PO
CHEBI_63036["CHEBI:63036"]
ing2_KH_PO -.-> CHEBI_63036
ing3_K2CO3["K2CO3"]
CultureMech_000091 --> ing3_K2CO3
ing4_MgSO__7H2O["MgSO .7H2O"]
CultureMech_000091 --> ing4_MgSO__7H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO__7H2O -.-> CHEBI_31795
ing5_Na_EDTA["Na EDTA"]
CultureMech_000091 --> ing5_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing5_Na_EDTA -.-> CHEBI_64734
ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000091 --> ing6_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000091 --> ing7_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
ing8_ZnCl2["ZnCl2"]
CultureMech_000091 --> ing8_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing8_ZnCl2 -.-> CHEBI_49976
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000091 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000091 --> ing10_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing11_Make_up_to_1_litre_with_deionised_water__Adjust_pH_to_6_8_with["Make up to 1 litre with deionised water. Adjust pH to 6.8 with"]
CultureMech_000091 --> ing11_Make_up_to_1_litre_with_deionised_water__Adjust_pH_to_6_8_with
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KNO
K.NO3 101.102 g/mol
|
0.25 G_PER_L |
CHEBI:63043
potassium nitrate
|
From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification |
| 2 | Ca(NO3)2.4H2O | 1.00 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification Name repaired (#276): the source string `Ca(NO ) .4H O` had lost its subscript digits; restored to `Ca(NO3)2.4H2O` — calcium nitrate tetrahydrate. | |
| 3 |
KH PO
H2O4P.K 136.084 g/mol
|
0.25 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification |
| 4 | K2CO3 | 0.345 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification Name repaired (#276): the source string `K CO` had lost its subscript digits; restored to `K2CO3` — potassium carbonate. | |
| 5 |
MgSO .7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Ontology mapping not yet available; From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification |
| 6 |
Na EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.75 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification |
| 7 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.097 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification |
| 8 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.041 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification |
| 9 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.005 G_PER_L |
CHEBI:49976
zinc dichloride
|
From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification |
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.002 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification |
| 11 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.004 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification |
| 12 | Make up to 1 litre with deionised water. Adjust pH to 6.8 with | 1 MOLAR | Ontology mapping not yet available; From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mhy (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: MHY, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_MHY.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.543172 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.136214Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000091 |
| 2026-03-17T19:45:04.091387+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MHY" to "mhy" for consistent matching |
| 2026-04-04T20:18:55.076899 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.535657+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.737191+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-06T03:04:20.145077+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b4a3496fadd91354088179f687651858ede4bd0752d1fba6444681a6246a51b9 |
| 2026-08-17T17:31:56.690265+00:00 | repair_subscript_stripped_names.py | REPAIRED_SUBSCRIPT_STRIPPED_NAME | Restored digits lost from formula ingredient name(s) by an upstream encoding step (#276): Ca(NO ) .4H O -> Ca(NO3)2.4H2O; K CO -> K2CO3. The corrupted original is preserved in each ingredient's notes. |