mhy (CultureMech:000091)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_MHY.pdf

Composition

flowchart LR
    CultureMech_000091["mhy"]
    ing0_KNO["KNO"]
    CultureMech_000091 --> ing0_KNO
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO -.-> CHEBI_63043
    ing1_Ca_NO3_2_4H2O["Ca(NO3)2.4H2O"]
    CultureMech_000091 --> ing1_Ca_NO3_2_4H2O
    ing2_KH_PO["KH PO"]
    CultureMech_000091 --> ing2_KH_PO
    CHEBI_63036["CHEBI:63036"]
    ing2_KH_PO -.-> CHEBI_63036
    ing3_K2CO3["K2CO3"]
    CultureMech_000091 --> ing3_K2CO3
    CHEBI_131526["CHEBI:131526"]
    ing3_K2CO3 -.-> CHEBI_131526
    ing4_MgSO__7H2O["MgSO .7H2O"]
    CultureMech_000091 --> ing4_MgSO__7H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO__7H2O -.-> CHEBI_31795
    ing5_Na_EDTA["Na EDTA"]
    CultureMech_000091 --> ing5_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing5_Na_EDTA -.-> CHEBI_64734
    ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000091 --> ing6_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000091 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_ZnCl2["ZnCl2"]
    CultureMech_000091 --> ing8_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing8_ZnCl2 -.-> CHEBI_49976
    ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000091 --> ing9_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000091 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (11)

#	Ingredient	Concentration	Ontology	Notes
1	KNO K.NO3 101.102 g/mol	0.25 G_PER_L	CHEBI:63043 potassium nitrate	From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification
2	Ca(NO3)2.4H2O	1.00 G_PER_L		Ontology mapping not yet available; From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification Name repaired (#276): the source string `Ca(NO ) .4H O` had lost its subscript digits; restored to `Ca(NO3)2.4H2O` — calcium nitrate tetrahydrate.
3	KH PO H2O4P.K 136.084 g/mol	0.25 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification
4	K2CO3 CO3.2K 138.204 g/mol	0.345 G_PER_L	CHEBI:131526 potassium carbonate	From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification Name repaired (#276): the source string `K CO` had lost its subscript digits; restored to `K2CO3` — potassium carbonate.
5	MgSO .7H2O 7H2O.Mg.O4S 246.473 g/mol	0.25 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Ontology mapping not yet available; From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification
6	Na EDTA C10H14N2O8.2Na 336.208 g/mol	0.75 G_PER_L	CHEBI:64734 EDTA disodium salt (anhydrous)	From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification
7	FeCl3 x 6 H2O Cl3Fe.6H2O 270.294 g/mol	0.097 G_PER_L	CHEBI:86254 iron trichloride hexahydrate	From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification
8	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.041 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification
9	ZnCl2 2Cl.Zn 136.296 g/mol	0.005 G_PER_L	CHEBI:49976 zinc dichloride	From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification
10	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.002 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification
11	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.004 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	From CCAP Medium MR_MHY.pdf; Curated from CCAP Medium MR_MHY.pdf specification

Preparation

Bring to 1 L with deionised water.
Adjust pH to 6.8 with 1 M NaOH or 1 M HCl before autoclaving.
Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	ccap-import	Imported from CCAP Culture Collection	Source ID: MHY, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_MHY.pdf
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added placeholder ingredients for missing composition
2026-02-03T11:26:43.543172	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.136214Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000091
2026-03-17T19:45:04.091387+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MHY" to "mhy" for consistent matching
2026-04-04T20:18:55.076899	batch_process_collection_media	EXPANDED_INGREDIENTS
2026-05-16T08:57:02.535657+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(4 keys) -> list(3 entries)
2026-05-16T09:02:56.737191+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-17T17:31:48.403858+00:00	repair_subscript_stripped_names.py	REPAIRED_SUBSCRIPT_STRIPPED_NAME	Restored digits lost from formula ingredient name(s) by an upstream encoding step (#276): Ca(NO ) .4H O -> Ca(NO3)2.4H2O; K CO -> K2CO3. The corrupted original is preserved in each ingredient's notes.
2026-08-20T19:00:48.242777+00:00	apply_mim_groundings.py	Adopted MIM published grounding	Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-08-25T00:00:00-07:00	repair_ccap_instruction_rows.py	REPAIRED_CCAP_INSTRUCTION_ROWS	Repaired preparation text and nested-recipe flattening against CCAP MR_MHY.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable.
2026-09-13T00:00:00-07:00	repair_ccap_sources_score10.py	NORMALIZED_CCAP_SOURCE_SCORE10	Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.