Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_TAP.pdf
Composition
flowchart LR
CultureMech_000139["tap_medium"]
ing0_CaCl_2H_O["CaCl 2H O"]
CultureMech_000139 --> ing0_CaCl_2H_O
ing1_MgSO_7H_O["MgSO 7H O"]
CultureMech_000139 --> ing1_MgSO_7H_O
ing2_KH_PO["KH PO"]
CultureMech_000139 --> ing2_KH_PO
CHEBI_63036["CHEBI:63036"]
ing2_KH_PO -.-> CHEBI_63036
ing3_Na_EDTA_2H_O["Na EDTA.2H O"]
CultureMech_000139 --> ing3_Na_EDTA_2H_O
ing4_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000139 --> ing4_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing4_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing5_H3BO3["H3BO3"]
CultureMech_000139 --> ing5_H3BO3
CHEBI_33118["CHEBI:33118"]
ing5_H3BO3 -.-> CHEBI_33118
ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000139 --> ing6_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
ing7_FeSO4_x_7H2O["FeSO4 x 7H2O"]
CultureMech_000139 --> ing7_FeSO4_x_7H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7H2O -.-> CHEBI_75836
ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000139 --> ing8_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000139 --> ing9_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
ing10_Make_up_to_1_litre_with_deionised_water__For_agar__add["Make up to 1 litre with deionised water. For agar, add"]
CultureMech_000139 --> ing10_Make_up_to_1_litre_with_deionised_water__For_agar__add
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | CaCl 2H O | 1 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification | |
| 2 | MgSO 7H O | 2 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification | |
| 3 |
KH PO
H2O4P.K 136.084 g/mol
|
5.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification |
| 4 | Na EDTA.2H O | 50 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification | |
| 5 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification |
| 6 |
H3BO3
H3BO3 61.833 g/mol
|
11.4 G_PER_L |
CHEBI:33118
boric acid
|
From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification Name repaired (#276): the source string `H BO` had lost its subscript digits; restored to `H3BO3` — grounded to boric acid (CHEBI:33118). |
| 7 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
5.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification |
| 8 |
FeSO4 x 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification |
| 9 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.6 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification |
| 10 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
1.16 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification |
| 11 | Make up to 1 litre with deionised water. For agar, add | 15 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification |
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- Chlorella vulgaris
- Term
- Id
- NCBITaxon:3077
- Label
- Chlorella vulgaris
- Strain
- UTEX 395
- Genome assembly id
- SAMN03453227
- Growth metrics
- Measurement conditions
- TAP medium laboratory co-culture condition comparing Chlorella vulgaris growth with and without Delftia sp.
- Evidence
- Reference
- PMID:39168352
- Supports
- SUPPORT
- Snippet
- Under laboratory conditions, we found that Delftia sp. had a negative effect on C. vulgaris growth when co-cultured in a TAP medium.
- Explanation
- The source explicitly reports Chlorella vulgaris growth behavior in TAP medium under laboratory culture conditions.
- Evidence
- Reference
- PMID:39168352
- Supports
- SUPPORT
- Snippet
- Chlorella vulgaris and bacteria of the genus Delftia
- Explanation
- The source studied Chlorella vulgaris UTEX 395 growth in TAP medium and compared algal growth with and without Delftia sp. co-culture.
Provenance
Merged from 1 source record.
- CCAP_TAP Medium (CultureMech:000139)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28 | ccap-import | Imported from CCAP Culture Collection | Source ID: TAP, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_TAP.pdf |
| 2026-02-03T11:23:39.722396 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.633751 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.266556Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000139 |
| 2026-03-17T19:45:04.214267+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "TAP Medium" to "tap_medium" for consistent matching |
| 2026-04-04T12:54:54.780497 | batch_process_collection_media | EXPANDED_INGREDIENTS | Replaced placeholder with 11 curated ingredients from CCAP Medium MR_TAP.pdf. |
| 2026-05-09T19:07:13Z | culturemech-agentic-curation | ADDED_GROWTH_EVIDENCE | Added Chlorella vulgaris UTEX 395 TAP growth evidence with NCBI Taxonomy, BioSample, and GenBank genome-project identifiers in references. |
| 2026-05-16T08:57:01.884460+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:55.919190+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-05-24T04:38:33.182473+00:00 | backfill_source_environment.py | BACKFILLED_SOURCE_ENVIRONMENT | added=2 env(s) from CommunityMech |
| 2026-08-06T00:58:02.505628+00:00 | curate_organism_culture_type.py | SET_ORGANISM_CULTURE_TYPE | Inferred isolate: 1 specific strain(s) named, no community signal (#142) |
| 2026-08-06T03:04:19.486210+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint cc63620b0e74caf9c04731133dc08757f35604da2baed02a3c5c2dfbedc2a389 |
| 2026-08-17T17:31:59.405174+00:00 | repair_subscript_stripped_names.py | REPAIRED_SUBSCRIPT_STRIPPED_NAME | Restored digits lost from formula ingredient name(s) by an upstream encoding step (#276): H BO -> H3BO3. The corrupted original is preserved in each ingredient's notes. |