tap_medium (CultureMech:000139)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_TAP.pdf

Composition

flowchart LR
    CultureMech_000139["tap_medium"]
    ing0_CaCl_2H_O["CaCl 2H O"]
    CultureMech_000139 --> ing0_CaCl_2H_O
    ing1_MgSO_7H_O["MgSO 7H O"]
    CultureMech_000139 --> ing1_MgSO_7H_O
    ing2_KH_PO["KH PO"]
    CultureMech_000139 --> ing2_KH_PO
    CHEBI_63036["CHEBI:63036"]
    ing2_KH_PO -.-> CHEBI_63036
    ing3_Na_EDTA_2H_O["Na EDTA.2H O"]
    CultureMech_000139 --> ing3_Na_EDTA_2H_O
    ing4_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000139 --> ing4_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing4_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing5_H3BO3["H3BO3"]
    CultureMech_000139 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000139 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_FeSO4_x_7H2O["FeSO4 x 7H2O"]
    CultureMech_000139 --> ing7_FeSO4_x_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7H2O -.-> CHEBI_75836
    ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000139 --> ing8_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000139 --> ing9_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing9_CuSO4_x_5_H2O -.-> CHEBI_31440

Ingredients (10)

#	Ingredient	Concentration	Ontology	Notes
1	CaCl 2H O	1 G_PER_L		Ontology mapping not yet available; From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification
2	MgSO 7H O	2 G_PER_L		Ontology mapping not yet available; From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification
3	KH PO H2O4P.K 136.084 g/mol	5.6 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification
4	Na EDTA.2H O	50 G_PER_L		Ontology mapping not yet available; From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification
5	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	22 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification
6	H3BO3 H3BO3 61.833 g/mol	11.4 G_PER_L	CHEBI:33118 boric acid	From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification Name repaired (#276): the source string `H BO` had lost its subscript digits; restored to `H3BO3` — grounded to boric acid (CHEBI:33118).
7	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	5.1 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification
8	FeSO4 x 7H2O Fe.7H2O.O4S 278.013 g/mol	5 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification
9	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	1.6 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification
10	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	1.16 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	From CCAP Medium MR_TAP.pdf; Curated from CCAP Medium MR_TAP.pdf specification

Preparation

Bring to 1 L with deionised water.
For an agar formulation, add 15 g/L Bacteriological Agar.
Autoclave at 15 psi for 15 minutes.

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

Preferred term
Chlorella vulgaris
Term
Id
NCBITaxon:3077
Label
Chlorella vulgaris
Strain
UTEX 395
Genome assembly id
- SAMN03453227
Growth metrics
- Measurement conditions
  TAP medium laboratory co-culture condition comparing Chlorella vulgaris growth with and without Delftia sp.
  Evidence
  Reference
  PMID:39168352
  Supports
  SUPPORT
  Snippet
  Under laboratory conditions, we found that Delftia sp. had a negative effect on C. vulgaris growth when co-cultured in a TAP medium.
  Explanation
  The source explicitly reports Chlorella vulgaris growth behavior in TAP medium under laboratory culture conditions.
Evidence
- Reference
  PMID:39168352
  Supports
  SUPPORT
  Snippet
  Chlorella vulgaris and bacteria of the genus Delftia
  Explanation
  The source studied Chlorella vulgaris UTEX 395 growth in TAP medium and compared algal growth with and without Delftia sp. co-culture.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28	ccap-import	Imported from CCAP Culture Collection	Source ID: TAP, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_TAP.pdf
2026-02-03T11:23:39.722396	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added placeholder ingredients for missing composition
2026-02-03T11:26:43.633751	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.266556Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000139
2026-03-17T19:45:04.214267+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "TAP Medium" to "tap_medium" for consistent matching
2026-04-04T12:54:54.780497	batch_process_collection_media	EXPANDED_INGREDIENTS	Replaced placeholder with 11 curated ingredients from CCAP Medium MR_TAP.pdf.
2026-05-09T19:07:13Z	culturemech-agentic-curation	ADDED_GROWTH_EVIDENCE	Added Chlorella vulgaris UTEX 395 TAP growth evidence with NCBI Taxonomy, BioSample, and GenBank genome-project identifiers in references.
2026-05-16T08:57:01.884460+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(4 keys) -> list(3 entries)
2026-05-16T09:02:55.919190+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	category->lowercase=1
2026-05-24T04:38:33.182473+00:00	backfill_source_environment.py	BACKFILLED_SOURCE_ENVIRONMENT	added=2 env(s) from CommunityMech
2026-08-06T00:58:02.505628+00:00	curate_organism_culture_type.py	SET_ORGANISM_CULTURE_TYPE	Inferred isolate: 1 specific strain(s) named, no community signal (#142)
2026-08-17T17:31:47.888710+00:00	repair_subscript_stripped_names.py	REPAIRED_SUBSCRIPT_STRIPPED_NAME	Restored digits lost from formula ingredient name(s) by an upstream encoding step (#276): H BO -> H3BO3. The corrupted original is preserved in each ingredient's notes.
2026-08-25T00:00:00-07:00	repair_ccap_instruction_rows.py	REPAIRED_CCAP_INSTRUCTION_ROWS	Repaired preparation text and nested-recipe flattening against CCAP MR_TAP.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable.
2026-09-13T00:00:00-07:00	repair_ccap_sources_score10.py	NORMALIZED_CCAP_SOURCE_SCORE10	Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.