modified_2x_chev_medium

Overview

Applications

• Algae cultivation
• Phytoplankton culture
• Microalgae research

Composition

flowchart LR
    CultureMech_000177["modified_2x_chev_medium"]
    ing0_1["1"]
    CultureMech_000177 --> ing0_1
    ing1_3["3"]
    CultureMech_000177 --> ing1_3

Ingredients (2)

Preparation

1. Important Notes To Consider: The quality of water, including natural seawater, is important.
2. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc.
3. Natural seawater and natural freshwater should be obtained from a non-polluted source. When dissolving chemicals, wait for the first component to dissolve before adding the second.
4. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently.
5. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts. Attention should be given to the pH of the final medium.
6. In most cases, if pH adjustment is required, this occurs before sterilization.
7. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers.
8. This would make the medium very alkaline soon after removal from the autoclave.
9. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• Modified_2X_CHEV_Medium (CultureMech:000177)