modified_2x_chev_medium

CultureMech:000177 algae COMPLEX LIQUID
Original name: Modified 2X CHEV Medium

Overview

External ontology
UTEX:modified-2x-chev-medium (source link unavailable) — Modified 2X CHEV Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares Modified 2X CHEV Medium by aseptically combining 200 mL prepared F/2 Medium with 800 mL sterile dH2O.

Composition

flowchart LR
    CultureMech_000177["modified_2x_chev_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000177 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
800 ML_PER_L CHEBI:15377
water
 UTEX lists 800 mL sterile dH2O per 1 L final medium.

Preparation

  1. Aseptically combine prepared F/2 Medium and sterile dH2O.
  2. Store at refrigerator temperature.

Solutions

F/2 Medium

Dose: 200 ML_PER_L

CultureMech:000170

UTEX lists 200 mL prepared F/2 Medium per 1 L final medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: modified-2x-chev-medium, URL: https://utex.org/products/modified-2x-chev-medium
2026-03-13T06:18:53.432648Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000177
2026-03-17T19:45:04.367173+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified 2X CHEV Medium" to "modified_2x_chev_medium" for consistent matching
2026-05-16T09:02:56.030512+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.235787+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.854129+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 2 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_remaining_score50.py RESOLVED_UTEX_SCORE50_ALGAE_GRAPH UTEX prepares Modified 2X CHEV Medium by aseptically combining 200 mL prepared F/2 Medium with 800 mL sterile dH2O.
2026-10-09T23:38:36.585055+00:00 codex CURATED_UTEX_CHEV_BATCH_INPUTS_AND_SCOPED_EVIDENCE L quantities are absolute input volumes per single-tube preparation, not final concentrations. Vessel capacity and summed liquid inputs do not establish final volume. The three fixed drop counts retain unresolved legacy VARIABLE encodings; their new evidence supports notes only. No drop-volume estimate is introduced. Modified CHEV's 1 mg/12 mL versus 0.5 mM source discrepancy remains unresolved. Its new evidence supports the discrepancy note, not the retained molarity. Referenced F/2 composition, chemical mappings, preparation, growth conditions and relationships are preserved, not newly certified. No new taxa-media associations.