Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Synonyms
-
- {'name': 'dasycladales_seawater_medium', 'source': 'unknown', 'original_category': 'algae'}
- {'name': 'euglena_medium', 'source': 'unknown', 'original_category': 'algae'}
Composition
flowchart LR
CultureMech_000242["waris_medium"]
ing0_1["1"]
CultureMech_000242 --> ing0_1
ing1_2["2"]
CultureMech_000242 --> ing1_2
ing2_3["3"]
CultureMech_000242 --> ing2_3
ing3_4["4"]
CultureMech_000242 --> ing3_4
ing4_5["5"]
CultureMech_000242 --> ing4_5
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 1 | variable G_PER_L | Original amount: Pasteurized Seawater | |
| 2 | 2 | variable G_PER_L | Original amount: Soil+Seawater Medium | |
| 3 | 3 | variable G_PER_L | Original amount: DAS Macro Solution | |
| 4 | 4 | variable G_PER_L | Original amount: Modified P-IV chelated Micronutrient Solution | |
| 5 | 5 | variable G_PER_L | Original amount: DAS Vitamin Cocktail |
Preparation
- Important Notes To Consider: The quality of water, including natural seawater, is important.
- The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc.
- Natural seawater and natural freshwater should be obtained from a non-polluted source. When dissolving chemicals, wait for the first component to dissolve before adding the second.
- Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently.
- Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts. Attention should be given to the pH of the final medium.
- In most cases, if pH adjustment is required, this occurs before sterilization.
- Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers.
- This would make the medium very alkaline soon after removal from the autoclave.
- In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- Dasycladales_Seawater_Medium (CultureMech:000161)
- Euglena_Medium (CultureMech:000168)
- Waris_Medium (CultureMech:000242)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | utex-import | Imported from UTEX Culture Collection | Source ID: waris-medium, URL: https://utex.org/products/waris-medium |
| 2026-03-13T06:18:53.709037Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000242 |
| 2026-03-17T19:45:04.611207+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Waris Medium" to "waris_medium" for consistent matching |
| 2026-05-16T09:02:56.179694+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1 |
| 2026-05-16T09:11:01.401673+00:00 | migrate_preparation_steps.py | MIGRATED_PREPARATION_STEPS | instruction->action+description for 9 step(s) |
| 2026-08-06T03:04:19.541314+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: Dasycladales_Seawater_Medium, Euglena_Medium, Waris_Medium |
| 2026-08-06T03:04:19.541357+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 32255c266d1c8f29bf1f1ab57357dd1b3be6859b03bab65cfcc07fcff6b3e00d |